Objective　To investigate the changes of free triiodothyronine (FT3) and its therapeutic effects on heart dysfunction after thoracic trauma.　 Methods　Eighteen rabbits were assigned randomly into 3 groups. The rabbits in Group A were served as a control, that in Group B were shot at the left thoracic wall passing through the cardiac region with small diameter rifle, that in Group C were treated by FT3 into veins (10 ng/kg) after thoracic trauma. 　Results　In Group B, MAP was much lower than that in Group A after trauma (P<0.05). In Group B, LVEDP and CVP respectively was much higher than that in Group A. Plasm concentration of cTnT in Group B was much higher than that in Group A. But level of FT3 was in Group B decreased more markedly than that in Group A after trauma. In myocardial interstitium, many red cells were leaked out of vessel. In Group C, MAP was much higher than that in Group B, no significant difference was found. CVP and LVEDP in Group C were much lower than that in Group B. Level of FT3 was near to normal and much higher than that in Group B. Edema of myocardial cells and hemorrhage of myocardial interstitium were alleviated markedly. 　Conclusions　Rabbits shot dead at the left thoracic wall passing through cardiac region with small diameter rifle can contribute to an animal model establishment of heart dysfunction. Reduction of FT3 is in correspondence with heart dysfunction. Correcting thyroid sick syndrome after trauma can improve significantly the heart function.

OBJECTIVE:To explore the effectiveness and safety of probucol combined with rosuvastatin in the treatment of vascu-lar dementia(VD). METHODS:In retrospective study,clinical information of 88 VD patients selected from neurology department of our hospital during May 2013-Feb. 2015 were divided into observation group and control group according to therapy plan,with 44 cas-es in each group. Both groups received conventional treatments such as controlling blood pressure and blood glucose,anticoagulation. Control group was additionally given Rosuvastatin calcium tablets orally 20 mg,at bedtime;observation group was additionally given Probucol tablet 0.5 g,bid,after meal,on the basis of control group. Both groups received treatment for consecutive 3 months. MMSE and ADL score were compared between 2 groups before and after treatment as well as the levels of TC,TG,LDL-C,HDL-C,CRP, TNF-α,IL-6,IL-1β,SOD and MDA. The occurrence of ADR was also observed. RESULTS:Before treatment,there was no statisti-cal significance in above indexes between 2 groups(P>0.05). Compared to before treatment,MMSE and ALD score,serum SOD lev-el of 2 groups were increased significantly,while serum levels of TC,TG,LDL-C,CRP,TNF-α,IL-6,IL-1βand MDA were de-creased significantly after treatment,with statistical significance(P<0.05). Above indexes of observation group were significantly bet-ter than those of control group,with statistical significance(P<0.05). There was no statistical significance in HDL-C level between 2 groups before and after treatment (P>0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05).CONCLUSIONS:Probucol combined with rosuvastatin is better than rosuvastatin alone in reducing blood lipid,serum inflamma-tory factors and oxidant stress indexes levels,improving cognitive function and quality of life with good safety.

Objective To observe the effect of Tiaozhi granule in treatment of hyperlipoproteinemia. Methods Randomized, blind and control trial was applied. Two hundred and forty hyperlipoproteinemia patients were randomly assigned to observation group (120 cases) and control group (120 cases). Observation group was treated with Tiaozhi granule, and control group with Xuezhikang. Two groups were treated for 4 weeks. Results Observation group and control group were improved in blood fats spectrum, TXB2, 6-Keto-PGF1?, and observation group was superior to control group (P

Objective To observe the effects of Panax notoginseng saponins and aspirin on platelet aggregation and analyzed resistance probability.Methods One hundred and twenty patients with cerebral infarction were di-vided randomly into Panax notoginseng saponins group and the aspirin group (each group 30 cases),respectively treated with adenosine diphosphatc (ADP),arachidonic acid (AA) to induced and detect the largest platelet aggre-gation (MAR).All patients received platelet aggregation test in hospital on the first and 14th day.Results In Pa-nax notoginseng saponins group and the aspirin group platelet aggregation induced by gents in the same role did not show statistical significance.There were resistances,semi-resistance phenomenon.Conclusions Panax notogin-seng saponins on platelet aggregation and the impact of aspirin was not significant; it may be used as anti-platelet aggregation drug,but there is also the resistance phenomenon.By combining Panax notoginseng saponins other an-ti-platelet drug anti-platelet aggregation of the long-term clinical.It might solve the resistance problem through large-scale clinical trials.

Objective To study the expression of brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) in the guinea pig cochlea of cisplatin-induced ototoxicity, and to understand its protective role for the inner ear.Methods The animals were divided into normal group and experimental group with cisplatin. Sodium chloride was used for the control. All animals were sacrificed on the day 3, 5 and 7, and the cochlea were used for BDNF and NGF staining.Results The weak expression of BDNF and the moderate expression of NGF were observed in the cochlea in the normal group. The strongest expression of BDNF and NGF was observed in the animals with cisplatin.Conclusion The existence of NGF in the normal cochlea indicates that NGF plays an important role to maintain auditory physiology.BDNF and NGF may be involved in self-protection mechanism for the cochlea and auditory nerves from cisplatin-induced ototoxicity.

Objective To observe the cerebral activation by functional MRI(F MRI) during the thumb movement in different motor fashion. Methods Four stroke patients with hemiplegia were involved in this study During the examination by F MRI, these patients were asked to move thumbs in passive, active and overcoming resistance fashion. Results (1) During the thumb at unaffected side moving in active fashion, functional activity was observed in the contralateral primary motor cortex In passive fashion, however, functional activity was observed in contralateral somatosensory cortex The overcoming resistance motor test was associated with activity in bilateral primary motor cortex and somatosensory cortex (2) The same motor tests were made in movement of the thumb at affected side , functional activity appeared not only in primary motor cortex, somatosensory cortex of affected side, but also in basal ganglia(affected area), frontal lobe, supplementary motor cortex, somatosensory cortex, and contralateral primary motor cortex. Conclusion These results have proved the neurorehabilitation theory such as neuroplasticity and so on

The effects of protein kinase C (PKC) inhibitors 1- (5-isoquinolinylsulfonyl) - 2-methylpoperazine (H-7) and quercetin on endotheliai-polymorphoneuclear (EC-PMN) adhesion induced by tumor necrosis factor (TNF) and platelet activating factor (PAF) were studied in cultured bovine pulmonary artery endothelial monolayers in vitro. TNF (100 U/ml) and PAF (1.0 ?mol/L) stimulated EC dependent PMN-EC adhesion. Both H-7 and quercetin dose-dependently inhibited TNF and PAF induced PMN-EC adhesion. The IC50 of H-7 was 22.22, 5.25 umol/L, and that of quercetin was 18.30, 4.83 ?mol/L respectively. WEB-2086, a specific PAF receptor antagonist, dose-dependently inhibited PAF induced PMN-EC adhesion, but had no effect on TNF induced adhesion. These results suggest that PKC play an important role in EC activation by TNF or PAF, and TNF induced PMN-EC adhesion by independent on endogenetic PAF.

Objective: To study the effects of herbimycin A on proliferation and NO production stimulated by IL 1 in chondrocytes. Methods: IL 1 alone or in combination with herbimycin A was administered.Chondrocytes proliferation was detected by crystal violet dying method and NO production was detected by Griess method. Results: IL 1 inhibited chondrocytes proliferation. Herbimycin A dose dependently reversed the effect. Also herbimycin A could inhibit NO production induced by IL 1. Conclusion: The reversing effect of herbimycin A on chondrocytes proliferation may be mediated by NO.

Objective To study the etiology, clinical features, risk factors of septic shock and multiple organ dysfunction syndrome /multiple organ failure (MODS/MOF) caused by gastroenteritis infection in the pediatric intensive care unit (PICU). Methods Case records of patients with gastroenteritis complicated by septic shock and MODS/MOF admitted to PICU in Children's hospital affiliated to Shanghai Jiaotong University from January 2000 to December 2004 were reviewed for etiology, case fatality rate, prognosis and relationship with MODS/MOF. Univariate analyses were performed to analyse the risk factors associated with septic shock and MODS/MOF. Results During the 5 years, 1 536 patients with critical illness were admitted and MODS/MOF associated with gastroenteriitis infection developed in 28 patients. The overall mortality of patients with MODS/MOF associated with gastroenteriitis was 75%. The mean age was (1.9?3.4)years and 19 cases(67.8%)were under 1 year. The patients with MODS/MOF involved (3.7?0.9)organs or systems on average. The numbers of involved organs systems were circulatory in 28 patients(100%), gastrointestinal system in 21(75%), lung in 20(71.4%), kidney in 14(50%),brain in 9(32.4%),blood in 9(32.4%) and liver in 5(17.9%). The first dysfunctional system was gastrointestinal tract in 13(46.4%),circulatory in 11(39.3%),and lung in 4(14.3%). The average volume of fluid resuscitation was (46.2?12.6)ml/kg and range from 30 ml/kg to 75 ml/kg in the first hour. The average fluid was (92.7?33.9)ml/kg and range from 70 ml/kg to 120 ml/kg in 6 hours. The cases fatality of patients with or without successful volume resuscitation were 66.7% (12 in 18 cases) and 90%(9 in 10 cases) in 6 hours respectively.Univariate analyses indicated the following risk factors: numbers of failed organ and lower pediatric critical illness score(P

Objective: To study the effect of lipopolysaccharides(LPS) on nitric oxide(NO) production in synoviocytes and inhibition of genistein on it.Methods: LPS or LPS+genistein stimulated synoviocytes in vitro .NO in culture medium was determined by Griess method.Results: LPS dose and time dependently induced NO production in synoviocytes; Genistein (6.25 50 ?mol/L) dose dependently inhibited the production of NO induced by lipopolysaccharide.Conclusion:The effect of LPS on NO production can be dose dependently inhibited by genistein,this may be mediated by tyrosine kinase(PTK).