AIM: To evaluate posterior capsular opacification (PCO) with hydrophobic acrylic intraocular lens (IOL, Sensar AR40e) and silicone IOLs after cataract surgery, to use a software program developed to provide an objective assessment of the amount of PCO in the digital images of the posterior capsule to quantify PCO. METHODS: Ninety-eight eyes underwent standardized phacoemulsification and "in the bag" IOL placement, were randomized to receive a three piece lens of hydrophobic acrylic or silicone, but lens materials were different in one case. In year 1 and 2, digitized retro-illumination images were taken from the posterior capsule. Images were analyzed by POCO software program, removing the Purkinje light reflexes, contrast enhancement, filtering to enhance low-density PCO. RESULTS: The percentage of PCO were 0.32±0.13 of hydrophobic acrylic IOLs in year 1, compared with 0.39±0.17 of silicone (P=0.37). In year 2, the percentage of PCO were 0.42±0.20 with hydrophobic acrylic IOLs and 0.34±0.18 with silicone IOLs (P=0.50). Of those patients with PCO in year 1 and 2, severity grades were 0.50±0.30 and 0.82±0.58 of hydrophobic acrylic cases, compared with 0.63±0.35 and 0.55±0.35 of patients with silicone IOLs (P=0.52,P=0.69) with no statistical significance.CONCLUSION: The POCO system is capable of producing an objective and repeatable measure of PCO that is relevant to assessing techniques of PCO prevention.

Objective To study the primiparas' sexual problems after delivery and the effect of in-dividualized nursing interventions on improvement of these problems in order to provide theoretic base-ment for sexual health education after delivery. Methods 248 primiparas after delivery in women's hos-pital were randomly divided into the intervention group and the control group with 124 cases in each group. All of them received self- designed questionnaires about sexual problems one year before pregnancy. The primiparas in two groups all receives routine care. Besides, the intervention group was given sexual health manual and received one-to-one individualized nursing intervention on sexual life after delivery according to their own conditions. The two groups were all received countercheck during 4 to 6 months after delivery and the results underwent X2 test. Results There was no significant difference between the sexual prob-lems one year before pregnancy for the two groups. But when calling back during 4 to 6 months after deliv-ery, compared with the control group, the intervention group had less sexual problems. Conclusions It is necessary to strengthen sexual health education in order to improve the sexual problems after delivery. Indi-vidualized nursing interventions can not only fulfill the sexual health demands of different primiparas after delivery, but also guarantee the women's privacy and safety.

BACKGROUND:Monofocal intraocular lens can restore distant vision of cataract patients, butit is not suitable fornear vision recovery, so most ofpatientsstil need to wear glasses. In contrast, multifocal intraocular lens can make up for the shortcomings of the single lenstoim provenear vision and increase the rate oftaking off the spectacles.
OBJECTIVE:To compare the visual function of senile cataract patients after aspheric multifocal and monofocal intraocular lens implantation.
METHODS:Totaly 30 senile cataract patients with 50 affected eyes were randomly divided into two groups: the eyes were implanted with aspheric multifocal and monofocal intraocular lens as experimental group and control group, respectively. At 3 months after implantation, distance visual acuity at a distance of 5 m, the best corrected distance visual acuity,as wel asnear and intermediate visual acuity at a distance of 30, 60 and 100 cm were detected, respectively. Besides, contrast sensitivity at a spatial frequency of 0.8, 1.5, 6 and 18 cpd under light and dark environment was measured.
RESULTS AND CONCLUSION:At 3 months after implantation, the distance visual acuity at a distance of 5 m in the two groups was significantly better than that before surgery (P< 0.05); no significant differences were found in near and intermediate visual acuitiesat a distance of 5 m and 100 cm between two groups, but the near visual acuitiesat a distance of 30 and 60 cm in the experimental group were significantly higher than those in the control group (P< 0.05). In addition, the contrast sensitivity at different spatial frequencies under light environment had no significant differences between two groups; and compared with the control group, contrast sensitivity at the frequency of 6 cpd under dark environment was significantly decreased in the experimental group (P< 0.05), but no significant differences were found in the other spatial frequencies. These results show that aspheric multifocal intraocular lens can significantly improve the near and intermediate visual acuity of senile cataract patients compared with the monofocal lens.

Objective To determine the effect of Vibrio vulnificus cytolysin (VVC) inducing ap-optosis in human umbilical vein endothelial cell(HUVEC) and its possible mechanism. Methods The en-tire vvhA gene that encoding VVC from V. vulnificus strain GTC333 was amplified by PCR and sequenced af-ter T-A cloning. E. coli BL21DE3pET-42a-vvhA, a prokaryotic expression system of the vvhA gene, was then con-structed. Ni-NTA affinity chromatography was applied to purify the target recombinant protein rVVC, and SDS-PAGE plus Bio-Rad Agarose Image Analyzor were used to measure the output of rVVC and to determine the purity of rVVC extract. The activity of rVVC dissolving rabbit erythrocytes was detected by hemolysis test. DPNH chromotometry and TphBNa chromotometry were performed to examine the contents of LDH and K+ in the supernatants of rVVC-treated HUVEC cultures, respectively. The effect of rVVC inducing apepto-sis of HUVEC was detected by flow cytometry, rVVC was labeled with FITC and the location of FITC-labe-ling rVVC in HUVEC was observed by laser canfocal microscopy. Results The cloned whA gene had 96.09% and 98.26% similarities of nucleotide and amino acid sequences compared to the corresponding se-quences in GenBank. rVVC, with a dosage of 1 μg/ml, could dissolve rabbit erythrocytes (P<0.01). 10 μg/ml rVVC was able to promote the increases of K+ content (P<0.01) but no change of LDH content could be found in the cell supernatants. HUVEC was apoptotic after the cell was treated with 1～100 μg/ml of rVVC for 2 h. In the 5～240 min duration of co-incubation of FITC-labeling rVVC and HUVEC, the rV-VC gradually moved from surface to inner side of the membrane and then entered the cytoplasms. When FITC-labeling rVVC treated HUVEC for 30 min, most of the rVVC was found to be intracellular location. Conclusion rVVC has cytolytic activity. VVC has an ability to enter HUVEC and causes injury of HUVEC via inducing apoptosis, which may be the major pathogenic mechanism of VVC.

OBJECTIVE To select the superior one from two skin-cleaning disinfection methods so as to reduce the possibility of hospital infection.METHODS The water plus soaps(control group) and Daniel disinfectants(test group) were used separately to clean and disinfect the skin of patients in intensive care unit(ICU).The degree of skin-cleaning of patients and hand pollution of nurses were observed and analyzed.RESULTS The number of bacteria on the skin of patients of the test group was remarkably lower than that of the control group with a statistically significant difference(t=7.94,P

0.05)respectively,when the whole radiotherapy was finished,the CR was 53.3% and 25.8%(P

Objective To develop an indicator system for evaluating midwifery quality. Methods Guided by the latest evidence-based framework of midwifery quality,based on indicator selection principle,the indicator system for evaluating midwifery quality was preliminarily developed by literature review,expert meeting,theoretical analysis and expert consultation,for further validation through large-scale Delphi method. Results The indicator pool for mid-wifery quality evaluation was identified by literature review,including indicator name and definition of special indi-cator. The indicator system was finalized through comprehensive analysis and selection,and consisted of 45 indica-tors. The expert consultation questionnaire response rate was 100%,the rate of experts who provided written sug-gestions was 81.3%,the authority coefficient was 0.92,the coefficients of variation of indicators ranged from 0 to 0.29. Conclusion The preliminarily developed indicator system for midwifery quality evaluation guided by the lat-est evidence-based framework of midwifery quality was scientific,oriented and accessible,which could be further ex-amined through large-scale Delphi method.

This article analyzes the factors of influencing practice quality from such aspects as hospital management,medical reform,clinical teachers and students and points out that only by enhancing the management of practice hospital as well as the system of examining and assessment,raising the teacher guidance and teaching consciousness and guiding the students to properly handle with the relations between employment,entrance exam for postgraduate and practice can we guarantee the clinical practice quality.

Adolescent ; Adult ; Head and Neck Neoplasms ; diagnosis ; pathology ; Humans ; Magnetic Resonance Imaging ; Male ; Meninges ; pathology ; Sarcoma ; diagnosis ; pathology ; Scalp ; pathology ; Skin Neoplasms ; diagnosis ; pathology ; Skull ; pathology

Vasohibin-1 (VASH1),which is induced in response to angiogenic stimuli such as vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)-2,has recently been isolated as a novel negative feedback inhibitor of angiogenesis.Several studies have demonstrated that VASH1 plays important roles in the development of various tumors and it would potentially be a biomarker and a candidate for molecular targeted therapy for patients with cancer in the future.