Periodontal ligament may have independent response to orthodontic stimulation and thus initiate alveolar bone osteogenesis and osteoclasts. Orthodontic-induced alveolar bone remodeling has no bone loss,while inflammation can lead to alveolar bone loss,suggesting that force signal and inflammatory factors may induce the differentiation of undifferentiated cells in the periodontal ligament via different pathways. The strength of the force and the nature of the force (basal tension and fluid shear force) may affect the differentiation of periodontal ligament cells,and may produce antagonistic or synergistic effect with the inflammatory factors through complex autocrine and paracrine regulation,resulting in local bone reconstruction,which is manifested as bone deposition and bone absorption. Studies have shown that Wnt signaling is an important regulatory pathway for osteoblast differentiation. Inflammatory factors can block the differentiation of stem cells into osteoblasts. The Wnt pathway is closely related to the effects of force and inflammatory factors on the differentiation of periodontal ligament cells.

Objective To investigate the effects of proprioceptive training and core stability training on the lower limbs motor function and balance in patients with hemiplegia after stoke. Methods 40 patients were randomly divided into treatment group (n=20) and control group (n=20). Both groups received routine rehabilitation and the treatment group received proprioceptive training and core stability training in addition. They were assessed with Fugl-Meyer Assessment (FMA) of lower limbs, Functional Ambulation Category (FAC), Berg Balance Scale (BBS), Timed Up-and-Go Test (TUGT) before and 6 weeks after treatment. Results The scores of FMA, FAC, BBS and TUGT improved in both groups after treatment (P<0.01), and improved more in the treatment group than in the control group (P<0.05). Conclusion Addition of proprioceptive training and core stability training may further improve the lower limbs motor function and balance of the hemiplegics after stroke.

Objective To observe the effect of comprehensive rehabilitation on breast cancer related lymphedema. Methods 70 patients with breast cancer related lymphedema were divided into experiment group (n=35) and control group (n=35). Both groups received intermittent pneumatic compression, while the experiment group received comprehensive rehabilitation including manual lymphatic drainage, muscle stretching, expanded the range of motion of shoulder, gradual resistance training, high frequency electrical therapy and health education. They were assessed with Disabilities of Arm, Shoulder and Hand Scale (DASH) and development of lymphedema before and 8 weeks after treatment. Results DASH scores and the development of lymphedema improved in both groups after treatment (P<0.05), and improved more in the experiment group than in the control group (P<0.05). Conclusion Comprehensive rehabilitation can further relieve breast cancer related lymphedema, and improve the upper extremity function.

Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; Hepatitis B ; immunology ; therapy ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; immunology ; Humans ; Immunotherapy

AIM:To investigate the effects of xeroderma pigmentosum D ( XPD) and p53 on the replication of hepatitis B virus ( HBV) .METHODS:Recombinant plasmid pEGFP-N2/XPD and vacant vector plasmid pEGFP-N2 were transfected into HepG2.2.15 cells by liposome.On the next day, these cells were incubated with pifithrin-α, a p53 inhibi-tor, at a concentration of 20 μmol/L for 24 h.The cells were divided into 5 groups: blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, pEGFP-N2/XPD+pifithrin-αgroup and pifithrin-αgroup.The mRNA expression of XPD, hepatitis B surface antigen ( HBsAg) , hepatitis B e antigen ( HBeAg) and hepatitis B virus X protein ( HBx) was detected by RT-PCR.The content of HBsAg and HBeAg in the supernatants of culture medium was measured by ELISA.The con-tent of HBV-DNA in the supernatants of culture medium was examined by fluorescence quantitative PCR.Using the method of bDNA, the content of HBV-DNA in the core particles was assessed.RESULTS:The expression of XPD mRNA was ele-vated by the transfection of recombinant plasmid pEGFP-N2/XPD.The increase in XPD expression significantly down-regu-lated the mRNA expression of HBsAg, HBeAg and HBx.The content of HBsAg and HBeAg in the supernatants of culture medium was significantly decreased by the increase in XPD expression.The results of fluorescence quantitative PCR showed that the content of HBV-DNA in the supernatants of culture medium was significantly down-regulated by the increase in XPD expression.bDNA results showed that the content of HBV-DNA in the core particles was significantly decreased by the increase in XPD expression.Pifithrin-αabolished the above-mentioned effects of XPD (all P<0.01).CONCLUSION:XPD inhibits the replication of HBV through p53 pathway.Therefore, XPD and p53 may be the targets for antiviral therapy of hepatitis B.

Objective To explore the design of pressure on breast for taking molybdenum target mammography after female's breast augmentation surgery.Methods The molybdenum target mammography in 26 patients were undergone breast augmentation surgery with hydrophilic polyacrylamide gel(HPAG) was performed under different breast pressure,the enduring ability on breast pressure for patients and the quality of axial and oblique images were compared between different pressure on breast.Results As the pressure on breast was 14 dan,the quality of images was not good,and the patients felt no pain on breast;the pressure was 18 dan,the quality of images was fine,and the pain on breast could be endured by patients.As the pressure was 20 dan,the quality of images was still fine,but the pain on breast was obvious.Conclusion It is suitable choosing the pressure of 18 dan on breast to take molybdenum target mammography for patients after breast augmentation surgery with HPAG.

Particulate matter is one of the important factors that have adverse effects on human health. It is generally known that the fine particles are more toxic than coarse ones, but some researchers have different opinion. Based on the analysis of the opinions, the factors that may influence the adverse effects of particulate matter on human health, including surface characteristics and adsorbed components, were discussed in the present paper. This paper will be helpful for the assessment of the toxicity of particulate matter.

High -resolution non -standard medical video roentgenoscoped by X -ray can't make use of universal video interfaces such as VGA, S - Video. Based on non - standard video feature transition algorithm library, high - speed field integration technology for FPGA digital system and VHDL hardware description language, this paper develops a real-time transition system for high-resolution non-standard medical video roentgenoscoped by X-ray.

Objective The experimental condition of PEG precipitation pre-treatment method associated with electrochemiluminescence was optimized to gain a coincident result compared with gel chromatography method.Methods A fixed centrifuge time,optimized centrifuge speed,temperature and iron intensity were used to achieve a best experimental condition.Results The detection concentration of micromolecular PRL was approximate to gel chromatography method under the experimental condition of being centrifuged 10 minutes at 18℃ and pretreated with 25%PEG 6000 1M PBS.Conclusions The optimized PEG precipitation test united with the electrochemillumescence immunoassy was a good method for screening macroprolactinemia in clinical diagnosis.

Objective To understand true feelings of patients with malignant tumor after implementing the protective medical treatment.In order to provide more humanized service for clinical nursing and to improve the job satisfaction of nurses.Methods The phenomenological methods of qualitative research was applied to carry out interviews with 12 patients to collected data.Then the data were analyzed.Results After reading,analysis,reflection,classification and refining the theme,we drew the following themes:(1)Before knowing diagnosis:half-believe and anxiety.(2) At the time of knowing diagnosis:luck,avoidance,acceptance and facing; (3) After knowing diagnosis:thirsting for information,low self-worth,improving self motivation.Conclusions We should not insist on hiding malignant tumor patients.Individualized and targeted measures should be taken to make patients cope with diagnosis better and improve the quality of their life.