It is a consensus to place stent after cutting bile duct in the hepatobiliary surgery in the past.However,as the development of bile physiological research and surgical technique,especially the raise of medical concepts of rapid recovery,the negative effects which are caused by the placement of stent have been taken seriously gradually.Up to now,whether the stent should be placed after the bile duct is cut has no definite answer yet.

Objective: To investigate the relationship among personality traits,motive of Internet use and tendency of Internet Addiction.Methods: Scale of Internet use,NEO-PI-R and Chinese Internet Addiction Scale(CIAS) were administrated to 207 university and high school students.Results: ①Tendency of Internet addiction was significantly correlated positively with openness(O)and agreeableness(A),and negatively with neuroticism(N).②Tendency of Internet addiction showed significant difference among motive of entertainment,study,information and intercommunication,and those who had higher tendency of Internet addiction would have stronger motive.③Internet addiction could be validly predicted by motive of information,entertainment and personality traits of neuroticism(N).Conclusion: Tendency of Internet addiction is affected by personality traits and motive of Internet use.

Balloon tamponade hemostasis has been applicated for 50 years,and its appropriate usage can obtain exact curative effect with less adverse reactions.In recent years,with the continuous improvement in surgical therapy,balloon tamponade technique in treating various types of bleeding has accumulated many successful experiences.This paper intends to review the advances in balloon compression technology for recent years.

Long-term outcomes of surgical bile duct injury (BDI) repair are affected by multiple factors, such as surgeon's experience, severity of BDI, pathological changes of the proximal duct, and the manner and timing of surgical repair. However, the timing of BDI repair is still controversial still. In this article, the authors reviewed the correlation of pathophysiologic changes of the proximal duct, repair timing and outcome discussed in English references. It was shown that pathophysiologic changes of the proximal duct are a major contributor to the timing of BDI repair.

Objective To evaluate the effect of omeprazole in preventing treatment of hypertensive cerebral hemorrhage with stress ulcer bleeding. Methods A total of 100 patients with hypertensive cerebral hemorrhage were treated with conventional therapy including dehydration, antihypertensive and supporting treatment. The patients were randomly divided into omeprazole group(n=50) and control group( n = 50). The control group received conventional therapyonly, while the omeprazole group received additional omeprazole 40mg, iv, qd, for 14d. Results Omeprazole group stress ulcer 6 eaess(12.0% ) was significantly lower than the control group 12 cases(24.0% );two groups stress ulcer incidence of severity are increasing with the increase of the disease; the mortalities of cerbral hemorrhage were 6.0 % in the omeprazole group and 16.0 % in the control group and the difference had significant meaning(P＞0.05). Conclusion Omeprazole has significant beneficial effect in preventing upper gastrointestinal hemorrhage after hypertensive cerebral hemorrhage.

0.05).Conclusion Under atherosclerosis conditions,CO_2 pneumoperitoneum has an adverse influence on the blood flow of the common carotid arteries which may be associated with increased intra-bdominal pressure,absorbed CO_2 gas.

[Objective]AIM To explore the differentiation of neural stem cells from rat embryonic spinalcord in the serum free condition culture medium of OECs.[Method]OECs were acquired by dissection ofolfactory nerve ensheathing and cultured for 20 hours,then suspending cells were put intoselective culture medium and cultured in the fibernectin coated plate.The activity of OECs was detected by MTT method on different periods and the best situation was selected out and serum-free cultured.Exudates of OECs was added into neural stem cells of the third generation.The differentiation of neural stem cells were observed under inverted microscope.The neural stemcells cultured were identified by immumofluorescence method.[Result]The activity of OECs was high on the 9~(th) day and the 12~(th) day.The serum free condition culture medium of OECs induced 53% neuralstem cells to differentiate into neuron-like cells and 42% neural stem cells into astrocytes cells.[Conclusion]The activity of OECs on different periods is different.The serum free condition culture medium of OECs can obviously induced neural stem cells to differentiate into mature neuronecells.

To investigate the value of tissue polypeptide specific antigen(TPS) in the diagnosis of hepatocellular carcinoma (HCC), the serum levels of TPS and alfa fetoprotein (AFP) were measured by TPS TM ELISA and enzyme immunoassay respectively in 96 patients with HCC, 30 patients with cirrhosis, 20 patients with hepatitis and 20 healthy controls.The diagnostic sensitivity of TPS and AFP is 89 6% and 73 0% respectively.There is a significant difference between them ( P

To investigate the expression of cytokeratin 18(CYK18) in hepatocellular carcinoma(HCC) cell membrane and the relationship between the serum levels of tissue polypeptide specific antigen (TPS) and the proliferating activity of HCC cells. The serum levels of TPS were determined by TPS TM ELISA in 96 patients with HCC. Four micrometer paraffin embeded sections of liver specimens from these patients were immunostained with the strept avidin biotin complex immunoperoxidase technique (IHC SABC). All of the HCC preparations were positively stained by CYK 18 and proliferating cell nuclear antigen (PCNA) with IHC SABC.The results of PCNA stainings were: + in 62 5%, in 26 0%,  in 8 3%,and  in 3 1% .The median levels of TPS in the four groups were: + 137 98 U/L,  685 3 U/L,  in 1 126 U/L and  4 672U/L respectively. There were significant differences among these groups ( P

Objective To explore the effects of CO2 pneumoperitoneum on pancreatic function in diabetic rabbits. Methods Forty-eight rabbits were divided into 4 groups: control group (the group of N0, n=4), the group of T0 (n=4), the group of T10 (n=20), and the group of T15 (n=20). The animal used in the groups of T0, T10 and T15 was diabetic rabbit, and the pressures of pneumoperitoneum of the three groups were 0 mm Hg, 10 mm Hg and 15 mm Hg respectively.The model of diabetic rabbits were made through intrvenous administration of Allxon. Arterial blood samples were collected before the onset of CO2 pneumoperitoneum, 0, 2, 6, 12 hours after deflation for measuring blood glucose, amylase, insulin and C-peptid. Then the rabbits were sacrificed and their pancreases were removed for measuring SOD activity and MDA content. Results After abdominal deflation, the blood glucose, amylase, insulin, C-peptid, MDA content were significantly increased (P