Objective:To establish a laser light scattering method for the determination of the particle size distribution of clostridium butyricum enterococcus triple viable powder and compare the results of the sieving method.Methods:The conditions of laser scattering method were as follows:the vibration sampling rate of 80%,the dispersion pressure of 0.05 MPa,the background and sample scan time of 15 s,the shading of 0.5%-5%,the refractive index of the particles of 1.55,the particle absorption rate of 0.01,and the injection volume of 0.1-0.2 g.The eigenvalues of the particle size distribution were determined,which were the particle size cumulative distribution map of 10%,50% and 90% of the particle size value and the volume average particle diameter D.Results:The RSDs of d(0.1),d(0.5) and d(0.9) were less than 5% in the methodology study.The results of laser light scattering method showed that the particle size of 93.3% samples was below 250 μm,that of 64.2% samples was below 150 μm,that of 51.4% samples was below 125 μm,and that of 31.3% samples was below 90 μm.The results of sieving method showed that the particle size of 96.6% was below 250 μm,that of 46.4% samples was below 150 μm,that of 23.5% samples was below 125 μm,and that of 1.4% samples was below 90 μm.Conclusion:Sieving method and laser light scattering method both can characterize the particle size distribution of the sample.The laser light scattering method is simple,accurate and producible,which is suitable for the particle size control of clostridium butyricum enterococcus triple viable powder.

Here are reported for the first time two newly established methods of semi-quantitative measurement of specific antigens by means of n microscope photometer in the tissures stained using non-labelled enzyme immunocyio-chemistry-the point and area scanning methods. We measured the content of vassoprcssin-like material in the vassopressinergic neurons of hypothalamus in the control and foot shocked rats with the two methods,respectively. The results prove them, to some extent, significant in studying the content of specific material in specific tissues (e.g. some nuclei), and even in single cells. In addition, a comparison between the two methods was conducted in this study.

Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Weaning

Bottle Feeding ; Breast Feeding ; Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Infant, Newborn ; Neonatology

Biomedical Research ; methods ; standards ; Disease ; etiology ; Humans ; Risk Factors

Americas ; Education, Medical ; standards ; Education, Medical, Continuing ; Germany ; Humans ; Internationality ; Internship and Residency ; Pediatrics ; education

Objective To assess the diagnostic value of DR,SCT,MSCT and MRI in diagnosis of osteoid osteoma.Methods Nineteen cases of osteoid ostcoma proved by surgical pathology were collected,Among the 19 cases,all patients had DR and CT scanning,8 with MR imaging.All the DR image processing were performed to display the nidus.Ten patients were examined by SCT axial scanning and 9 cases were performed by MSCT using isotropic parameters,then multi-direction MPR were performed.The ability of DR,SCT,MSCT and MKI in demonstrating the nidus and the surrounding reaction were analyzed.Results Among 19 cases.only 6 cases showed nidus on DR before image processing and 12 showed nldus after image processing.All cases with CT scanning showed nidus.Among 8 cases with MRI.only 6 eases could be diagnosed correedy.The nldns could be ariarmed in only 4 cases.and the nidus was affirmed by comparing with DR or CT in 2 eases,the other 2 cases were misdiagnosis.Among 10 eases with SCT,only 6 cases showed calcification of nidus.Whereas among 9 cases with MSCT,8 cases showed calcification of nidus.There were also soft tissue and bone marrow edema around the nidus on MR imaging in all 8 cases.Conclusion Nidm is the key in diagnosing the osteoid osteoma correctly.DR is a basic checking method for osteoid osteoma.DR image processing con improve the display rates of nidus.CT scan is the best method to demonstrate the nidus.MSCT is better than SCT in demonstrating the nidus and the surrounding reaction.MRI can demonstrate the soft tissue and bone marrow edema around the nidus sensitively.but probably lead to an incorrect diagnosis.Combining with DR or CT.MRI can make an accurate diagnosis.

Objective To study preoperative nursing intervention mode for thyroid surgery and its effect.Methods 78 patients were randomly divided into two groups.39 patients in the intervention group received psychological care 30 minutes before operation besides preoperative interview 24 hours before operation,while 39 patients in the control group received routine visit 24 hours before operation.Indexes including the values of blood pressure,heart rate and the SCOre of anxiety of the two group patients were measured and recorded before interview and before anesthesia.Results There were no difference in the observational indexes between the two groups before interview(P＞0.05),while after intervention(before anesthesia),the values of blood pressure and heart rate and the Score of anxiety in the intervention group were lower than those in control group (P＜0.01).Conclusion Preoperative systematic interview combined psychological care before operation is an effective nursing intervention mode,it can help patients who will receive thyroid surgery reduce the fluctuation of blood pressure and heart rate,and it can also relieve the preoperative anxiety.

Animals ; Calcium ; metabolism ; Copper ; metabolism ; Environmental Exposure ; prevention & control ; Erythrocytes ; metabolism ; Hazardous Substances ; metabolism ; poisoning ; Humans ; Mercury ; metabolism ; Metallothionein ; biosynthesis ; genetics ; metabolism ; Zinc ; metabolism

Child ; Child Health Services ; organization & administration ; China ; Health Care Sector ; standards ; trends ; Humans ; Patient Care Team ; standards ; trends ; Primary Health Care ; organization & administration