Here are reported for the first time two newly established methods of semi-quantitative measurement of specific antigens by means of n microscope photometer in the tissures stained using non-labelled enzyme immunocyio-chemistry-the point and area scanning methods. We measured the content of vassoprcssin-like material in the vassopressinergic neurons of hypothalamus in the control and foot shocked rats with the two methods,respectively. The results prove them, to some extent, significant in studying the content of specific material in specific tissues (e.g. some nuclei), and even in single cells. In addition, a comparison between the two methods was conducted in this study.

Objective:To establish a laser light scattering method for the determination of the particle size distribution of clostridium butyricum enterococcus triple viable powder and compare the results of the sieving method.Methods:The conditions of laser scattering method were as follows:the vibration sampling rate of 80%,the dispersion pressure of 0.05 MPa,the background and sample scan time of 15 s,the shading of 0.5%-5%,the refractive index of the particles of 1.55,the particle absorption rate of 0.01,and the injection volume of 0.1-0.2 g.The eigenvalues of the particle size distribution were determined,which were the particle size cumulative distribution map of 10%,50% and 90% of the particle size value and the volume average particle diameter D.Results:The RSDs of d(0.1),d(0.5) and d(0.9) were less than 5% in the methodology study.The results of laser light scattering method showed that the particle size of 93.3% samples was below 250 μm,that of 64.2% samples was below 150 μm,that of 51.4% samples was below 125 μm,and that of 31.3% samples was below 90 μm.The results of sieving method showed that the particle size of 96.6% was below 250 μm,that of 46.4% samples was below 150 μm,that of 23.5% samples was below 125 μm,and that of 1.4% samples was below 90 μm.Conclusion:Sieving method and laser light scattering method both can characterize the particle size distribution of the sample.The laser light scattering method is simple,accurate and producible,which is suitable for the particle size control of clostridium butyricum enterococcus triple viable powder.

Objective To investigate the diagnostic value of 64-slice CT and MRI in detection of abnormal sacroiliac joint changes in patients with early stage of ankylosing spodylitis (AS).Methods The 64-slice CT and MRI imaging data of the sacroiliac joint early diseases(grade 0 ～ Ⅱ lesion) for the 66 patients with clinical confirmed were reviewed retrospectively,of which MRI contrast enhanced was performed in 6 patients.Results CT showed grade zero lesion 15 cases,grade Ⅰ lesion in 20 cases and grade Ⅱ lesion in 31 cases.MRI revealed bilateral articular bone marrow edema in 52 cases,articular cartilage erosive destruction in 48 cases,fat accumulation of bilateral articular bone in 36 cases,articular bone erosive destruction in 31 cases.Bilateral synovial membrane enhancement was showed in all patients who underwent contrast enhanced.Conclusions MRI can detect acute inflammatory changes which can not display by MSCT.Compared with MSCT,MRI is more useful in detection of abnormal sacroiliac joint changes in patients with early stage of AS.

Objective To assess the diagnostic value of DR,SCT,MSCT and MRI in diagnosis of osteoid osteoma.Methods Nineteen cases of osteoid ostcoma proved by surgical pathology were collected,Among the 19 cases,all patients had DR and CT scanning,8 with MR imaging.All the DR image processing were performed to display the nidus.Ten patients were examined by SCT axial scanning and 9 cases were performed by MSCT using isotropic parameters,then multi-direction MPR were performed.The ability of DR,SCT,MSCT and MKI in demonstrating the nidus and the surrounding reaction were analyzed.Results Among 19 cases.only 6 cases showed nidus on DR before image processing and 12 showed nldus after image processing.All cases with CT scanning showed nidus.Among 8 cases with MRI.only 6 eases could be diagnosed correedy.The nldns could be ariarmed in only 4 cases.and the nidus was affirmed by comparing with DR or CT in 2 eases,the other 2 cases were misdiagnosis.Among 10 eases with SCT,only 6 cases showed calcification of nidus.Whereas among 9 cases with MSCT,8 cases showed calcification of nidus.There were also soft tissue and bone marrow edema around the nidus on MR imaging in all 8 cases.Conclusion Nidm is the key in diagnosing the osteoid osteoma correctly.DR is a basic checking method for osteoid osteoma.DR image processing con improve the display rates of nidus.CT scan is the best method to demonstrate the nidus.MSCT is better than SCT in demonstrating the nidus and the surrounding reaction.MRI can demonstrate the soft tissue and bone marrow edema around the nidus sensitively.but probably lead to an incorrect diagnosis.Combining with DR or CT.MRI can make an accurate diagnosis.

Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Infant, Low Birth Weight ; Infant, Newborn ; Infant, Premature

Objective To investigate the changes of human leukocyte antigen-G (HLA-G) protein expression and Th1/Th2 type cytokines in intrahepatic cholestasis of pregnancy (ICP) and their relativity to the etiology of ICP. Methods Peripheral blood and placental tissues were obtained from 26 ICP patients (the ICP group) and 22 normal pregnant women (the NP group) in the operation room for Cesarean birth. Immunohistochemistry was used to detect the expression of HLA-G protein in the placental tissues. Meanwhile we tested the concentrations of tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) by enzyme-linked immunosorbent assay (ELISA) in the peripheral blood and checked the levels of TBA in the serum.Results TBA level in the ICP group was (27.05±6.08) μmol/L, significant higher than that in the NP group (4.35±2.68)μmol/L (P＜0.01). The positive expression of HLA-G protein in extravillous trophoblast in the ICP group was significantly lower than that in the NP group (P＜0.01). The mean optical density (MOD) of positive expression of HLA-G protein in the placenta tissues in the ICP group (52.91±7.19) was significantly lower than that in the NP group (69.26±7.72) (P＜0.01). The concentration of TNF-α was significantly higher in the ICP group (101.31±19.30) pg/mL than that in the NP group (54.51±23.72) pg/mL (P＜0.01). The concentration of IL-4 was lower in the ICP group (22.16±6.55) pg/mL than that in the NP group (31.69±8.25) pg/mL (P＜0.01). The ratio of TNF-α/IL-4 was higher in the ICP group (4.52±1.91) than that in the NP group (1.72±0.61) (P＜0.01). There was a negative correlation between the MOD of HLA-G protein and TNF-α (r=-0.98, P＜0.01) in the ICP group. No correlation with IL-4 and TNF-α/IL-4 was seen (P＞0.05). There was a positive correlation between TBA and TNF-α (r=0.99, P＜0.01), and a negative correlation between TBA and the MOD of HLA-G protein (r=-1.00, P＜0.01) in the ICP group. No correlation with IL-4 and TNF-α/IL-4 was seen (P＞0.05). Conclusion There is an imbalance of Th1/Th2 cytokines to the Th1 type in the peripheral blood of ICP patients. The expression of HLA-G protein in the placenta of ICP patients decreases, leading to an increase of Th1 type cytokines that may be one of the reasons for liver destroy in ICP.

Child ; Humans ; Nutritional Sciences ; trends ; Pediatrics

Child ; Child Development ; physiology ; Humans

Humans ; Liver, Artificial ; Translational Medical Research

Child ; Education, Medical, Graduate ; Humans ; Nutritional Sciences ; statistics & numerical data ; trends ; Pediatrics ; Statistics as Topic