Here are reported for the first time two newly established methods of semi-quantitative measurement of specific antigens by means of n microscope photometer in the tissures stained using non-labelled enzyme immunocyio-chemistry-the point and area scanning methods. We measured the content of vassoprcssin-like material in the vassopressinergic neurons of hypothalamus in the control and foot shocked rats with the two methods,respectively. The results prove them, to some extent, significant in studying the content of specific material in specific tissues (e.g. some nuclei), and even in single cells. In addition, a comparison between the two methods was conducted in this study.

Objective:To establish a laser light scattering method for the determination of the particle size distribution of clostridium butyricum enterococcus triple viable powder and compare the results of the sieving method.Methods:The conditions of laser scattering method were as follows:the vibration sampling rate of 80%,the dispersion pressure of 0.05 MPa,the background and sample scan time of 15 s,the shading of 0.5%-5%,the refractive index of the particles of 1.55,the particle absorption rate of 0.01,and the injection volume of 0.1-0.2 g.The eigenvalues of the particle size distribution were determined,which were the particle size cumulative distribution map of 10%,50% and 90% of the particle size value and the volume average particle diameter D.Results:The RSDs of d(0.1),d(0.5) and d(0.9) were less than 5% in the methodology study.The results of laser light scattering method showed that the particle size of 93.3% samples was below 250 μm,that of 64.2% samples was below 150 μm,that of 51.4% samples was below 125 μm,and that of 31.3% samples was below 90 μm.The results of sieving method showed that the particle size of 96.6% was below 250 μm,that of 46.4% samples was below 150 μm,that of 23.5% samples was below 125 μm,and that of 1.4% samples was below 90 μm.Conclusion:Sieving method and laser light scattering method both can characterize the particle size distribution of the sample.The laser light scattering method is simple,accurate and producible,which is suitable for the particle size control of clostridium butyricum enterococcus triple viable powder.

Biomedical Research ; methods ; standards ; Disease ; etiology ; Humans ; Risk Factors

Americas ; Education, Medical ; standards ; Education, Medical, Continuing ; Germany ; Humans ; Internationality ; Internship and Residency ; Pediatrics ; education

Child ; Child Welfare ; Clinical Trials as Topic ; Female ; Humans

Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Weaning

Bottle Feeding ; Breast Feeding ; Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Infant, Newborn ; Neonatology

Breast Feeding ; Consumer Product Safety ; Humans ; Infant ; Infant Food ; Nutritional Physiological Phenomena ; Triazines ; toxicity

Child ; Child Nutrition Disorders ; etiology ; physiopathology ; therapy ; Child Nutritional Physiological Phenomena ; Digestive System Diseases ; complications ; physiopathology ; Europe ; Gastroenterology ; Humans ; Nutritional Support ; Pediatrics

OBJECTIVE:To provide references and information about the research and development,manufacture and use for the related departments.METHODS:The sales amount,sequence of sales and etc.of the Chinese Traditional Medicine in our hospital from1999to2003were analyzed statistically with the help of database and electronic table software.RESULTS:Percentage of sales amount of the Chinese Traditional Medicine has been lowered from43.81%to33.70%.The top5drugs on the consumption list were the blood-regulating agents,tonifying agentss,drugs for bone fractures and wounds,febrifugal ag ents and gynaecological drugs respectively;the blood-regulating agents had a big share on the top10consumption volume list.CONCLUSION:There is a decrease in the consumption of the Chinese Traditional Medicine.