Objective To investigate the characteristics of apparent diffusion coefficient (ADC)values in the normal fetal kidney and to explore the normal data reference of fetal kidney by using diffusion-weighted imaging (DWI).Methods 71 normal kidneys of fetuses confirmed by ultrasound and MR were examined with DWI with four b values of 100 s/mm2 ,200 s/mm2 ,500 s/mm2 and 700 s/mm2 ADC values of the symmetrical ROIs were compared between left and right kidney for each b value.While ADC values between different group in ipsilateral kidney were compared between different b values.And the relationship between ADC values and gestational age was also evaluated.Results When b = 100 s/mm2 ,there was a statistically significant difference between the ADC value of left and right kidney,and the remaining group showed no statistically significant differences between the ADC values of left and right kidney.Comparisons among different b values of ipsilateral kidney showed statistically significant differences among the groups with different b values except the comparison between b=100 s/mm2 and b=700 s/mm2 groups of the right kidney with the increase of gestational weeks,the ADC values of bilateral kidneys were declining,and there was a reflection of this trend in different b values. Conclusion It is feasible for using DWI to evaluate the fetal kidney development.With a selected different b value,ADC value is a specific quantitative parameter that could help evaluating normal fetal kidney development.

Objective: In this paper, a method of gas chromatography mass spectrometry for determination of free resveratrol in grape wine was established. Methods: After evaporated to eliminate ethanol, sample of grape wine was extracted with ethyl acetate, and evaporated again to dryness. The residues were derivatized with N,O bis (trimethylsianyl) trifluoroacetamide plus 1% trimethychlorosilane. Then, qualitative and quantitative analysis of cis and trans resveratrol were carried out using GC MS scan mode. Results: The average recoveries were 85.6%-110.5%,and the relative standard deviations were 8.2%-11.5%, when resveratrol was added to grape wine at 0.25-1.25 mg/L levels. The correlation coefficient was 0.995 in range of 0.50-10.00 mg/L. The detection limit for resveratrol in grape wine was 0.005 mg/L. Further 15 grape wines made in China were analyzed. The contents of cis resveratrol were 0.02 -0.56 mg/L and trans resveratrol were 0.15-1.28 mg/L. Total resveratrol were 0.26-1.48 mg/L. Conclusion: This assay is accurate and reproducible for determination of free resveratrol in grape wines.

Colorectal cancer is a common malignancy with an annual death of 0.5 millions of people worldwide. Currently the most accepted method of treating colorectal cancer is operation. With the popularization of the concept of multidisciplinary treatment, the research of chinese medicine in the treatment of colorectal cancer is furthered. More and more studies show that chinese medicine can prevent or reduce adverse effect of radiotherapy and chemotherapy, and can also reduce postoperative tumor recurrence and metastasis, so as to prolong the life span and improve life quality. Although much has been achieved, many questions have yet to be solved. Clinical research with chinese medicine only is less, which cannot reflect the principle of treatment based on syndrome differentiation; the credibility and generalizability of chinese medicine were restricted because of the absent of standard effectiveness evaluation system; high quality, large sample, multi-center, randomized double-blind clinical researches need to be carried out.

Objective To explore the relationships between personality traits and internet addiction disorder, as well as which traits would affect internet addiction, and to provide the psychology basis against internet disorder in teenage. Methods The Cattell 16 Personality Factor Questionnaire (16PF) and Chinese Internet Disorder Scale were applied to 409 undergraduates and high school students in Changsha. Use SPSS for windows 11.0 to carry on statistical processing. Results (1) Rule consciousness personality trait certain forecast internet disorder total score ( t = 3.18 ), withdrawal factor ( t = 4.17 ), tolerant factor ( t = 2.64) and time manage factor ( t =3.01 ). (2) Abstractedness personality trait certain forecast internet disorder total score ( t = 2.47 ) and interpersonal and healthy factor ( t = 3.08 ). (3) Warmth personality trait certain forecast forced-internet behavior factor ( t = 2.14 ) and time manage factor ( t = 2.41 ). (4) Social Boldness personality trait certain forecast interpersonal and healthy factor ( t = 2.42). Conclusion Personality traits certain influence internet addiction disorder.

Objective To investigate the relationship between the expression of VCAM-1 and oncogenesis, tumor angiogenesis and metastasis in breast carcinoma. Methods Using semi-RT-PCR and immunohistochemistry technique, specimens from 63 patients of breast cancer,20 of fibroadenoma were detected for expression of VCAM-1.Microvessel density(MVD) was counted by endothelial cells immunostained using monoclonal antibody CD34.Circulating sVCAM-1 concentrations were measured by enzyme linked immunosorbent assay. Results Of 63 breast cancer tumor tissues VCAM-1 mRNA was detected in 37 cases. The rate of VCAM-1 mRNA in the primary breast cancer tissues were significantly higher than that in the adjacent non-cancerous tissues and benign fibroadenoma tissues (?2=43,P

Objective To investigate the value of 18F-FDG PET-CT in the diagnosis of adrenal metastasis.Methods 88 patients (107 adrenal lesions) with adrenal metastasis and benign tumor underwent 18F-FDG PET-CT,all adrenal lesions' short diameter,CT value and 18F-FDG maximum standardized uptake values (SUVmax) were measured.The t test,chi-square test and Pearson correlation test were used for data analysis.Meanwhile,receiver operating characteristic (ROC) curve was used to determine the ability of CT value and SUV~x to differentiate the adrenal metastasis and benign tumor.Results There were 73 adrenal metastases and 34 adrenal benign tumors of 107 adrenal lesions.The short diameter of adrenal metastasis was (2.17±1.14) cm,benign tumor was (1.76±1.00) cm,there was no statistical significant difference between the two groups (t =1.817,P =0.072).The CT value of adrenal metastasis and benign tumor were (29.65±10.29) Hu and (14.83±14.42) Hu (t =5.389,P =0.000),respectively.The SUVmax of adrenal metastasis was 9.28±5.33,while benign tumor was 2.81±1.14,statistical significant difference was found between the two groups (t =9.890,P =0.000).For adrenal metastasis,a significant positive correlation was found between short diameter and SUV~ (r =0.620,P =0.000),and it was also found between CT value and SUVmax (r =0.561,P =0.000).Among all patients with adrenal metastasis,32 (58.2 ％) patients' lesions were found in the left adrenal gland,and 5 (9.1％) in the right side (x2 =29.689,P =0.000).ROC curve analysis showed that using lesion' s SUVmax ＞ 4.1 and CT value ＞ 20 Hu as diagnostic criteria,the sensitivity,specificity,accuracy,PPV and NPV were 91.3 ％ (63/69),94.7 ％ (36/38),92.5 ％ (99/107),96.9 ％ (63/65) and 85.7 ％ (36/42),respectively.Conclusion 18F-FDG PET-CT has high sensitivity,specificity and accuracy in diagnosis of adrenal metastasis,and the adrenal lesion' s SUVmax ＞ 4.1 and CT value ＞ 20 Hu is a better diagnostic criteria.

The peroneal artery and its relation to the blood supply of the fibula was studied in 100 Chinese adult cadavers. The results were as follows:1. The length of fibula ranged from 29.8 to 40.0 cm with an average of 34.26 ?0.23 cm.2. In the 100 cases, about 90.0?3.0% of the peroneal artery originated from the posterior tibial artery; 1.0?0.99% from anterior tibial artery; and 1.0?0.99% from the popliteal artery; and 8.0?2.71% the peroneal artery replaced the posterior tibial artery.3. The external diameter of the origin of the peroneal artery ranged from 0.15 to 0.6 cm with an average of 0.37?0.01 cm.4. The place where the peroneal artery comes to a distance of 0.5 cm from the fibula was taken as the approach point. The distance of this point to the apex of fibular capitulum varied from 6.30 to 17.01 cm with an average of 10.35?0.22 cm5. There were 94 fibular nutrient arteries in which, two were found in doublets. No fibular artery was found in 8 cases. Its length ranged from 0.4 to 2.0 cm with an average of 0.9?0.07cm.6. 51.06?5.0% of the fibular nutrient foramina were found on the posterior of fibula, where it is the most commonly located.7. There were 1～5(more frequently 3～4)branches of the arch artery supplying the periosteum and bone cortex.8. The muscular branches supplied the posterior and lateral muscle groups, their numbers varied greatly.9. According to its origin, the peroneal artery may be grouped into four types. The most common type was those came from the posterior tibial artery (90.0?3.0%).10. The peroneal artery was discussed with reference to the blood supply of fibula. It points out the importance of the middle/upper, lower/upper, upper/middle and middle/middle segments of this artery in surgery.

The use and management of medical implanting material is always the key point of the hospital material management. Questions existing in hospital management were investigated along with some suggestions in further management in the system and the process.

A method for isolation and primary culture of guinea pig alveolar type Ⅱ epithelial cells is reported. The injurious effects of phospholipase A2 (PLA2) on the cultured cells were studied. Trypsinizing solution was instilled into the bronchoalveolar space. The lungs were digested, sectioned, vortexed and filtered. The yield of original cell mixture was 172.3?106 cells/guinea pig, of which 33.6% were type Ⅱcells. At 48 h of culture following short term selective attachment 7.9?106 cells were obtained, of which 66.1% were type Ⅱ ceils. The purity could further be raised to 76.2% (63.9% -92%) if pretreated with ethyienediarnine tetraacetic acid (EDTA) before trypsinization to remove attached cells. The characteristics of cultured cells were observed under phase contrast microscopy, light microscopy and electron microscopy. After exposure of the cells to 300U/ml PLA2 for 4h at 37℃, detachment and lactic dehydrogenase (LDH) release of the cultured cells increased significantly. It is suggested that PLA, may damage and promote detachment of cultured type Ⅱ cells and it may play an important part in the development of some diseases.

Objective To investigate the role of PD-L1 in the pathogenesis of acute allograft rejection (AR) and its correlation with PD-1 and the degree of tubulointerstitial lesions. Methods A total of 20 patients admitted by our hospital and Daping hospital from 1999 to 2003 were included, 12 male and 8 female, aged 18-40 years old (average 32.2?4.5). All of them were diagnosed as acute rejection by renal biopsy. Fifteen specimens from the mismatched donor kidney and the normal tissues from the resected kidney served as normal control. Immunohistochemistry was used to detect the expression of PD-L1 and PD-1 in renal tissues of AR and normal control. Results Both PD-L1 and PD-1 expression were significantly higher in AR than that in normal control. The intensity of PD-L1 positive in renal tissues of AR showed a positive correlation with PD-1 expression and a negative correlation with the degree of tubulointerstitial lesions. Conclusion Increased expression of PD-L1 and PD-1 in renal tissues of AR has an important effect on the degree of tubulointerstitial lesions.