1.Synergistic Effect of Nucleotide binding Oligomerization Domain 2 and Toll-like Receptor Agonists on Proliferation and Release of Proinflammatory Cytokines in Vascular Smooth Muscle Cells
Chinese Journal of Hypertension 2006;0(12):-
Objective To investigate the expression of nucleotide-binding oligomerization domain 2(NOD2),an intracelluar pathogen pattern recognition receptor,and Toll like receptor(TLR) 2,4 in human vascular smooth muscle cells(VSMC),and its effect on production of proinflammatory cytokines in VSMC.Methods Human coronary artery smooth muscle cells were in vitro stimulated with NOD2 agonist Muramyl dipeptide(MDP),TLR2 agonist Pam3CSK4(PAM3)and TLR4 agonist lipopolysaccharides(LPS) alone or MDP in cocultured with either PAM3 or LPS.The mRNA expression of NOD2 and fibroblast growth factor-2(FGF-2) were measured by real time RT-PCR.The concentration in the culture supernatants of interleukin-8(IL-8) and tumor necrosis factor-?(TNF-?) was determined by ELISA.VSMC proliferation was analyzed by the MTT assay.Results MDP up-regulate the expression of NOD2 mRNA in VSMC in a time-dependent manner(0 h:0.028?0.001;3 h:0.045?0.002;6 h:0.053?0.002;24 h:0.162?0.013).It up-regulate the expression of FGF-2 mRNA(MDP 9.3?0.4 vs control 7.4?0.2;P
2.Role of nucleotide binding oligomerization domain receptor 1 as a new innate immune recognition receptor in activation of vascular smooth muscle cells
Chinese Journal of Tissue Engineering Research 2007;0(11):-
AIM: Nucleotide binding oligomerization domain receptor 1 (NOD1) is a recently identified intracellular pathogen pattern recognition receptor of innate immunity. This study was designed to explore the role of NOD1-mediated innate immune signal pathway in the activation of vascular smooth muscle cells (VSMCs), and investigate the effect of peptidoglycan (PGN) on the activation and expression of NOD1 in human VSMCs. METHODS: The experiment was performed in the central laboratory of the Second Affiliated Hospital of Dalian Medical University from June 2006 to March 2007.①Subject: Human coronary artery VSMCs were purchased from Cambrex company.② Methods: Human coronary artery VSMCs were cultured in vitro, and stimulated with NOD1 agonist PGN (10 mg/L) for 0, 3, 6 and 24 hours. ③ Evaluation: The mRNA expression of NOD1 in VSMCs was measured by real time quantitative reverse transcription-polymerase chain reaction. The concentration in the culture supernatants of interleukin-8 (IL-8) and tumor necrosis factor-? (TNF-?) was determined by enzyme linked immunosorbent assay. RESULTS: ①Human VSMCs constitutively expressed a low level of NOD1 at resting condition. Upon PGN stimulation, the expression of NOD1 mRNA was up-regulated in VSMCs, from 0.164?0.005 to 0.231?0.027 (P
3.Analysis of Antidepressants Used in Our Hospital During the Period 1998~2001
China Pharmacy 1991;0(04):-
OBJECTIVE:To get clear idea about the current situation and future trend of use of antidepressants METHODS:By using the methods of order of sum of money and DDDs,the antidepressants used in our hospital in years 1998~2001 were analysed RESULTS:The annual sum of money and DDDs of antidepressants were increasing and the sum of money and DDDs of antidepressants used in 2001 were 3 69 times and 2 14 times as much as those in 1998,respectively Although the proportion of sum of money of tricyclic antidepressants(TCAs) was lower than that of new type antidepressants(NTAs),Clomipramine hydrochloride and Doxepin belonging to TCAs were in the first five places in order of DDDs during the past four years Because of the low price and less side effect,the position of NTAs in therapy of depressions was increasing CONCLUSION:TCAs are still the first choice in therapy of depressions The prospects of NTAs will be wide and bright It is important to speed up the development of Chinese materia medica in therapy of depressions
4.The intracellular pattern recognition receptors-NOD like receptors
Chinese Journal of Pathophysiology 2000;0(12):-
NODs are cytosolic proteins that contain a nucleotide-binding oligomerization domain(NOD).These intracellular proteins are a class of pattern recognition receptors with unique functions in the innate and the acquired immune systems.NOD like receptors,including NOD1 and NOD2,are associated with host responses to intracellular invasion by bacteria or the intracellular presence of specific bacterial products.Activation of NOD like receptors initiates proinflammatory signalling via NF-?B activation,which is necessary for clearance of infecting pathogens from the host.Several different mutations in the genes encoding NOD1 and NOD2 are associated with susceptibility to inflammatory disorders.
5.Diagnosis and treatment of carcinoid in a case.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(7):606-607
Aged
;
Carcinoid Tumor
;
diagnosis
;
therapy
;
Humans
;
Laryngeal Neoplasms
;
diagnosis
;
therapy
;
Male
6.TCM Data Management Based on Object Characteristics under the Background of Big Data
Changsong DING ; Haoyu QU ; Shiwen WU
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(9):10-14
Targeting the problem that it is difficult for traditional data management mode to effectively manage TCM information, this article analyzed from the aspect of object characteristics of TCM information resources and summarized that the reason lies in the TCM information resources with big data 4V features, proposed strategies for TCM information resource management based on big data technology, and expounded the feasibility.
7.Chemical constituents of Galla Chinensis
Chunyuan LI ; Weijia DING ; Guirong QU
Chinese Traditional and Herbal Drugs 1994;0(08):-
Objective To study the chemical constituents of the petroleum and chloroform extracts of Galla Chinensis.Methods Using chromatography on silica gel column and thin-layer chromatography to isolate and purify the compounds;using spectroscopy methods to elucidate their structures;using double dilution to screen antibacterial activities on Fusarium oxysporum.Results Ten compounds were isolated and identified as 2-hydroxy-6-pentadecyl benzoic acid(Ⅰ),4-(pentadec-8-enyl) phenol(Ⅱ),plamitic acid-1,3-dipropyl ester(Ⅲ),?-sitosterol(Ⅳ),pentacosane(Ⅴ),4-hydroxy-3-methoxy-benzoic acid(Ⅵ),plamitic acid(Ⅶ),lauric acid(Ⅷ),myristic acid(Ⅸ),and gallic acid(Ⅹ).Conclusion Compounds Ⅰ-Ⅵ are obtained from Galla Chinensis for the first time.Compound Ⅰ shows moderate inhibitory activity on F.oxysporum.
8.Research progress of neutrophil extracellular traps and related targeted drugs for the treatment of inflammatory diseases
Acta Pharmaceutica Sinica 2021;56(3):669-678
The inflammatory response is an essential role of innate immune cells such as neutrophils, which plays an important role in the occurrence and development of inflammatory diseases. Neutrophil extracellular traps (NETs) are responsible for killing microorganisms and inducing the inflammatory response. We review the function of NETs in inflammatory diseases based on research publications since 2016. In addition, the ability of drugs that target NETs to ameliorate inflammation-related diseases is summarized. This review suggests a new strategy of targeting NETs for the treatment of inflammation-related diseases.
9.A prospective comparison of shape memory alloy embracing fixator and reconstruction plate fixa-tion in treatment of midshaft clavicle fractures
Guoxin QU ; Genyuan CHEN ; Xuanxi DING ; Shouzheng LIU
Chinese Journal of Trauma 2010;26(1):64-68
Objective To compare the clinical outcome of shape memory alloy (SMA) embra-cing fixator and reconstruction plate fixation in treatment of displaced or comminuted midshaft clavicle fractures. Methods There were 65 patients with displaced or comminuted midshaft clavicle fractures surgically treated with either a SMA embracing fixator or a reconstruction plate. The patients were divided into two groups according to fixation methods, ie, SMA embracing fixator group (30 patients at average age of 28.1 years) and reconstruction plate group (35 patients at average age of 26.1 years). All pa-tients were followed up for mean 12 months and shoulder function was evaluated using shoulder score. Results Compared with reconstruction plate group, SMA embracing fixator had shorter operation time, smaller wound incision and less loss blood (all P < 0.05). However, there was no statistical difference in aspects of hospital stay, cost, JOA shoulder score and bone union rate between two groups (all P > 0.05). Conclusion The internal fixation with SMA embracing fixator is better than reconstruction plate fixation in treatment of midshaft clavicle fractures.
10.Maternal serum tumor necrosis factor-? concentration and correlation with insulin resistance in gestational diabetes
Shenglan WANG ; Peiqiu LIU ; Yu DING ; Wei PENG ; Xin QU
Chinese Journal of Obstetrics and Gynecology 2000;0(11):-
Objective To investigate se rum concentration of tumor necrosis factor(TNF)-? and its correlation with insulin resistance in pregnant women with gestational diabetes mellitus (GDM). Methods Enzyme-linked immunosorbent assay was used to measure th e fasting serum TNF-? levels of 42 women with GDM (28~39 gestational weeks), a n d 40 cases of normal pregnant women in the third trimester. Fasting plasma gluc ose, insulin, C-peptide and glycosylated hemoglobin (HbA1c) were also measured at the same time. Insulin sensitive index (ISI) was calculated. Res u lts (1)Significantly elevated serum TNF-? was found in the women w i th GDM(5.2?1.6) ng/L as compared with the healthy pregnant women in third t rimester (4.5?0.5)ng/L(P