Objective To investigate the role of RhoA and Rho kinase system in the onset of labor. Methods Forty term pregnant women, who delivered through cesarean section at the Xiangya Second Hospital of Central South University from February 2007 to November 2007, were selected and divided into 2 groups: 20 in labor group and 20 in non-labor group. Another 20 non-pregnant women undergoing hysterectomy due to cervical intraepithelial neoplasia were chosen as the control. Real-time fluorescence quantitative RT-PCR and western blot were applied to detect the expression RhoA and ROCKⅠ mRNA and protein in uterine smooth muscle tissue and the correlation between the mRNA and protein expression of RhoA and ROCKⅠ were analyzed. Results (1) The mRNA expressions of both RhoA and ROCKⅠ were detected in all groups, and higher levels were found in the labor group than in the non-labor group and the control [RhoA mRNA: (3.51±0.56)×10-3 vs. (2.75±0.52)×10-3 and (2.11±0.54)×10-3; ROCKⅠ mRNA: (4.07±0.66)×10-3 vs. (2.71±0.52)×10-3 and(2.01±0.23)×10-3, P<0.01]. (2) RhoA and ROCKⅠ proteins were also identified in all three groups, and the expressions in the labor and non-labor group were higher than those of the control (RhoA protein: 0.72±0.23 and 0.64±0.17 vs. 0.46±0.15; ROCKⅠ protein: 0.56±0.14 and 0.42±0.16 vs. 0.29±0.08, P<0.01). (3) The expression of RhoA mRNA and ROCKⅠ mRNA were positively correlated in each of the three groups (r=0.73,P<0.01), and the same was found in the expression of RhoA protein and ROCKⅠ protein (r=0.37,P<0.01). Conclusion The increased expression of RhoA and Rho kinase may play an important role in the initiation of labor.

CMTM3 and CMTM7 are two newly discovered anti-oncogenes,which exist in various kinds of organs and tissues and having multiple biological functions including cell proliferation,differentiation,migration,and apoptosis. Recently,it has been shown that these two anti-oncogenes have closely relationship with gastrointestinal tract tumor,which can inhibit the growth of esophageal,gastric,and colorectal cancer. In this review article,the advances in study of the relationship between these two anti-oncogenes and tumors of gastrointestinal tract were summarized.

To study the clinicopathological features of breast carcinoma with responsive granuloma. MethodsFive cases of breast carcinomas with responsive granuloma were investigated by morphological observation. ResultsAll the five patients had noncaseating epithelioid granulomas. Multinucleated giant cells, predominantly of Langhans type, were present in granulomas. The granulomas were restricted to the carcinoma, and no responsive granulomas were evident in the regional lymph nodes. None of the patients had clinical evidence of systemic granulomatous disease. ConclusionDifferential diagnosis of breast carcinoma with responsive granuloma should include the carcinoma with osteoclast-like giant cells, metaplastic the carcinoma with osteoclastic giant cells, granulomatous lobular mastitis, stromal responsive giant cells, and carcinoma with sarcoidosis or tuberculosis of the breast.

Objective:To compare performances of Wechsler Memory Scale(WMS)and Clinical Memory Scale(CMS),to explore applicability of WMS and CMS in the different degree traumatic brain injured patients.Methods:206 patients were tested with WMS and 300 with CMS,and completed clinical diagnosis for all,to compare the performances.Results: ①There was no significant differences in the MQ between the two groups in the normal、marginal memory impairment, there were significant differences in the mild memory impairment.②There were remarkable variance between the MQ and the clinical diagnosis,WMS was even more variant.Conclusion:①There is similar outcome between WMS and CMS in the patients with normal、marginal memory impairment,CMS shows more applicable in the patients with more severe mem- ory impairment.②It is not correct to diagnose memory defect degree with MQ without more clinical information.

Objective To assess the imaging characters and clinical applications of triphasic spiral computer tomography(CT) scanning in ureteral lesions.Methods We analyzed of 76 consecutive cases of clinically evidenced ureteral lesions using the cross-sectional images and CTU derived from triphasic spiral CT scanning retrospectively.Results Twetny-four cases of 76 ureteral lesions patients were diagnosed as ureteral tumor,resulting in severe urinary tract obstruction.The shape,size,number,regional ureter wall inflammatory infiltration of visible mass were verified as well.Twetny-six cases were caused by ureteral stones.The "edge" sign was shown clearly.Ten cases were ureteral inflammation,characterized by different ureteral wall thickening and enhancement feature.Eight cases were ureteral tuberculosis that secondary to renal tuberculosis.Uneven ureteral wall thickening,stiffness and mild to moderate density were shown in CT scanning.Ureteral congenital malformations were found in 3 patients.The other five cases were caused by exogenous factors.CT scanning could identify the mass general profile.Conclusion Triphasic spiral CT scanning plays an important role in ureteral lesions diagnosis and differentiation from other similar diseases.

Objective To assess the imaging characters and clinical applications of triphasie spiral computer tomography (CT) scanning in ureteral lesions. Methods We analyzed of 76 eonseeutive eases of elinieaUy evidenced ureteral lesions using the cross - sec-fional images and CTU derived from triphaaie spiral CT scanning retrospectively. Results Twetny - four cases of 76 ureteral lesions pa-tients were diagnosed as ureteral tumor, resulting in severe urinary tract obstruction. The shape, size, number, regional ureter wall in-flammatory infiltration of visible mass were verified as well. Twetny- six cases were caused by ureteral stones. The "edge" sign was shown clearly. Ten cases were ureteral inflammation, characterized by different ureteral wall thickening and enhancement feature. Eight cases were ureteral tuberculosis that secondary to renal tuberculosis. Uneven ureteral wall thickening, stiffness and mild to moderate densi-ty were shown in CT scanning. Ureteral congenital malformations were found in 3 patients. The other five cases were caused by exogenous factors. CT scanning could identify the mass general profile. Conclusion Triphasic spiral CT scanning plays an important role in ureteral lesions diagnosis and differentiation from other similar diseases.

Objective To use three different methods-different nursing level,different ADL and nursing manhour to allocate nursing human resources,and find their difference.Methods Using observation method to record nursing time,and then using three different methods to allocate nursing human resources.Results 22 persons were needed using nursing level to allocate,20 persons were needed using ADL method and 15 persons were needed using nursing manhour to allocate nurses.The difference of direct nursing time between patients with primary care and secondary care had statistically significant difference.The difference of direct nursing time between patients with different ADL had statistically significant difference.Conclusions It is different using three methods to allocate nursing human resources.How to select a suitable method to accurately calculate nursing human resources and combine computer and the present methods is the problem which we should solve currently.

Objective To establish a new inhibitory kinetic spectrophotometric method for the determination of trace Sb3+ in water sample. Methods A new inhibitory kinetic spectrophotometric method for the determination of trace Sb3+ was established based on the inhibitory effect of Sb3+ on the oxidation of thionine by H2O2 in a buffer solution of NH3-NH4Cl at pH=10.50. Results The linear range of this method was 0.004-0.40 ?g/ml, r=0.999 2,the limit of detection was 1.71?10-3 ?g/ml, and RSDs were 1.4%～ 2.1% , the recovery rates were 96.2% -101.9% . Conclusion This method is accurate, fast, simple and applicable to the determination of antimony content in environment water samples.

[Objective] To evaluate the efficacy of reinforcing short-segment pedicle screw fixation with posterior morselized bone grafting in vertebrae for spinal fusion in patients with thoracolumbar vertebrae fractures.[Method]Seventy patients with thoracolumbar vertebrae fractures were treated with short-segment pedicle screw fixation.Fractures in Group A(n =20) were reinforced with posterior morselized bone grafting in vertebrae for spinal fusion.Group B patients(n =50) were not treated with morselized bone grafting.Kyphotic deformity,anterior vertebral height,instrument failure rates,and neurological function outcomes were compared between the two groups.[Result]Kyphosis correction was achieved in Group A(morselized bone grafting) and Group B(Group A,6.4 degrees,Group B,5.4 degrees).At the end of the follow-up period,kyphosis correction was maintained in Group A but lost in Group B(Group A,0.33-degree loss,Group B,6.20-degree loss)(P=0.0001).After surgery,greater anterior vertebral height was achieved in Group A than in Group B(Group A,12.9%,Group B,2.3%)(P

The regional blood flow measurement (RBFM)of lenghtened limb with a peripheral circulation mesuremnt system was carried ou t in 20 rabbits after their tibia was lengthened in different lengthening rates.It was found that whenever a limb was lengthened with a rate over 1 mm/day,the RBFM of the lengthened limb decreased rapidly and returned to preoperative level 8 hours later.It is believed that the vascular and muscular tissues of a limb possess good adaptive capcity to the lengthening rate of 1 mm/day,When the lengthening rate exceeds 1 mm/day,it is liable to result in a significant re-duct of regional blood flow,and then atrophy and weakening of the muscles will occur.