Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

Objective To explore the effect of rehabilitation training on negative symptom of patients with schizophrenia. Methods 70schizophrenics were divided into control group (n=35) and observation group (n=35). Both groups took antipsychotics. The control group receivedroutine psychiatric nursing, and the observation group received targeted rehabilitation training additionally. The rehabilitation effectwas assessed by Scale for Assessment of Negative Symptoms (SANS) and Inpatient Psychiatric Rehabilitation Outcome Scale (IPROS). ResultsThe scores of lack of interest, attention dysfunction, ability of daily life, social function, concern and interest were lower in observationgroup than in control group (P<0.05). Conclusion Rehabilitation training during hospitalization can improve the social function of schizophrenics.

Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium (RPE) cells,Müller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.

Objective To observe the early efficacy and safety of combined sequential administration of lamivudine and interferon alfa-2b in patients with hepatitis Be antigen (HBeAg)-positive chronic hepatitis B. Methods 71 patients were divided into combined therapy growp (23 eases),interferon group (27 cases) and lamivudine group (21 cases).patients in the first group were given sequential combination treatment with of lamivudine monotherapy for nine weeks followed by lamivudine plus interferon alfa-2b for 15 weeks while patients in the interferon group and Lamivudine group received interferon alfa-2b monotherapy and lamivudine respectively. Results At 24weeks, 43.37% of the patients who received sequential combination treatment and 33.33% of those who received interferon alfa-2b monotherapy had HBeAg seroconversion,which is higher than those who received lamivudine monotherapy(9.52%, P0.05). The normolization rates of ALT in combining group , interferon group and Lamivudine group were 60.87%, 51.85% and 19.04% respectively(P

Neonatal necrotizing enterocolitis(NEC) is a common critical disease of the digestive system in neonatal period.In severe case,it may even endanger the lives of newborn.The mechanism of NEC is complex and uncertain.Neonatal infection is one of the most important aspects of the mechanism.The immature digestive system,intestinal barrier structure and function in premature infants,the innate defects of intestinal immunity and the abnormal intestinal bacterial colonization can lead to a high incidence of NEC in premature infants.Currently,there is limited specific treatment.It will benefit for reducing incidence of NEC if prevention is targeted on the found of risk factors.

Objective To investigate the antioxidant activity of diferent ethanol elution fractions on macroporous resin from Kochia scoparia.in vitro.Methods 70% ethanol extract from Kochia Scoparia.was further isolated and purified by AB-8 macroporous resin into the five elution fractions(DFZ1, DFZ2, DFZ3, DFZ4, DFZ5),then the total flavonoids of diferent elution fractions were determined by ultraviolet spectrophtometry.The scavenging rate of the five elution fractions to superoxide anion radical and hydroxyl radical and reducing power were determined.Results The total flavonoids contents of DFZ1, DFZ2, DFZ3, DFZ4 and DFZ5 reached(0.82±0.02)%,(8.09±0.66)%,(10.31±0.73)%,(2.73±0.09)%,(1.88±0.06)% respectively.The total antioxidant activity increased with the increasing concentration of total flavonoids.Conclusion From the results it could be concluded that the five elution fractions from Kochia Scoparia had strong antioxidant capacityin vitro.

Child ; Evidence-Based Medicine ; Glomerulonephritis, IGA ; diagnosis ; therapy ; Guidelines as Topic ; Humans

Humans ; Infant ; Infant Food ; Infant, Newborn ; Triazines ; toxicity ; Urinary Calculi ; chemically induced