Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

Child ; China ; Humans ; Pediatrics ; Periodicals as Topic ; standards ; statistics & numerical data ; Publishing ; standards ; statistics & numerical data ; Quality Control

Female ; Humans ; Melanosis ; Middle Aged ; Occupational Diseases

Child ; Child, Preschool ; China ; epidemiology ; Evidence-Based Medicine ; Humans ; Kidney Diseases ; diagnosis ; epidemiology ; therapy ; Practice Guidelines as Topic

Bartter Syndrome ; complications ; Diarrhea ; etiology ; Female ; Humans ; Infant ; Male ; Syndrome

Humans ; Infant ; Infant Food ; Infant, Newborn ; Triazines ; toxicity ; Urinary Calculi ; chemically induced

Child ; Evidence-Based Medicine ; Glomerulonephritis, IGA ; diagnosis ; therapy ; Guidelines as Topic ; Humans

ObjectiveTo evaluate the role of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for diagnosing lesions of gastrointestinal tract and the nearby organs. MethodsClinical and pathological data of 161 EUS-FNA lesions in 133 consecutive patients were retrospectively analyzed. ResultsOf the 161 lesions, there were 142 solid lesions, 15 cystic lesions and 4 pancreatic solid-cystic lesions. EUS-FNA was performed at lesions of upper gastrointestinal tract, rectum and anal canal. 160 cytologic diagnoses (99. 4％ ) were obtained in all 161 lesions. 143 histological diagnoses (97.9％) and 113 immunohistological diagnoses (77.4％) were obtained in 146 solid lesions (including 4 pancreatic solid-cystic ones). Diagnosis rates of samples for immunohistology remained similar between 22-gauge and 19-gauge needles and also similar among different sites (P ＞ 0. 05). But immunohistological diagnosis rate of smaller lesions ( ＜ 1 cm) was lower than that of bigger ones ( ≥ 1 cm) ( P ＜ 0. 05). The diagnosis rates of cytology,histology and pathology in solid lesions were 81.2％ ( 120/146), 82. 9％ (121/146) and 89. 7％ ( 131/146), respectively. Size of needles and lesion location did not exert influence on diagnosis, but the diagnosis rate of smaller lesions ( ＜ 1 cm) was lower than that of bigger ones ( ≥ 1 cm). Cytological diagnosis was consistent with histological diagnosis ( k =1. 0, P =0. 00), 11 cases that could not be diagnosed by cytology was confirmed by histology and 10 cases that could not diagnosed by histology was confirmed by cytology.There were no complications during the procedures. ConclusionEUS-FNA is a valuable diagnostic tool for lesions of gastrointestinal tract and nearby organs. The needle size and lesion site were not influential factors for diagnosis. Smaller lesions ( ＜ 1 cm) yielded lower diagnosis rate than bigger ones ( ≥ 1 cm). The cytologic diagnosis combined with histology can improve the diagnostic value of EUS-FNA.