Objective:To elucidate the effect of osteogenic growth peptide(OGP) on the collagen I in cultured rat osteoblast-like cells.Method:Osteoblast-like cells were treated with 10-11~10-7mol/L of OGP for 7d respectively.Then cells were processed for SABC stain and examination of the collagen I.Microscopy and analysis system was used to investigate the changes of collagen I.Result:OGP can promote the expression of collagen I in rat osteoblast-like cells.The most best concentration of OGP is 10-9mol/L.Conclusion:Our results demonstrate that OGP at 10-9mol/L may stimulate the expression of collagen I in rat osteoblast-like cells in vitro.

OBJECTIVE: To investigate the drug use involved in outpatient's prescriptions in a hospital in 2007.METHO-DS: With the International Selected Drug Use Indicators and defined daily dose as indexes,the drug use involved in a total of 5 029 outpatient's prescriptions randomly selected from a hospital were analyzed statistically based on Excel sheet.RESULTS: Of the total 5 029 outpatient prescriptions,60.83% paid the expenses at one's own expense,and the average age of the patiens were 46.9 years.The average number of drugs prescribed for patient's each visit was 2.63 kinds,and the percentages of antibacterial agents,ampuls,and prescriptions of drugs prescribed in generic names were 21.79%,10.44%,and 50.47%,respectively.Quinolones,cephalosporins and macrocyclic lactones ranked at the first three places in DDDs and consumption sum.The drug utilization indexes of cephalosporins and penicillins were on the high side.23.72% of antibacterial agents used in combination and 76.28% alone,20.07% in bigeminy.The antibacterial agents used in trigeminy or more than 3 kinds in combination were all for antiuberculotic treatment.Physician's signature rate after prescription revision was 60.19%,and the rate of prescriptions in which medicines for oral administration or for external use were prescribed alone was 32.88%.CONCLUSION: The drug use for the outpatients in the hospital is rational on the whole,but measures should be taken to tighten control on the drug use by strenthening physicians' consiousness on rational drug use so as to reduce the incidence of irrational drug use.

Neurodegenerative diseases,which are common nervous system diseases,seriously affect people''s life quality and even lives. So the pathogenesis of neurodegenerative diseases is attracting more and more attention in recent years. People gradually realize that endoplasmic reticulum stress is associated with the pathogenesis of many diseases through studying the function of the endoplasmic reticulum. Neurodegen-erative diseases, endoplasmic reticulum stress,unfolded protein responsewere used as the key words of retrieval performance in the databases such as Pubmed,CNKI and so on. And the papers which closely re-lated with the theme were chosen to investigate the association between alzheimer''s disease,parkinson''s dis-ease,Huntington'' s disease ,amyotrophic lateral sclerosis and ERS. It turned out that the expression of mo-lecular chaperone GRP78/Bip was increased in all neurodegenerative diseases, and the phosphorylation of eIF2α increased in most neurodegenerative diseases. However, the expression of some ERS-related factors was completely different in different neurodegenerative diseases. Thus,the study of ERS may be an important breakthrough for the pathogenesis and treatment of neurodegenerative diseases.

Dizziness ; classification ; epidemiology ; Humans ; Vertigo ; classification ; epidemiology

Objective To investigate the mental health and the general well-being among Hangzhou scientific and technical workers,and the mediating effect of coping strategies in perceived level of stress and mental health status.Methods A stratified cluster sampling method were developed among engineering technicians,agrotechnicians,researchers,health works and teaching staff.510 participants were evaluated with perceived stress of different types,Trait Coping Style Questionnaire (TCSQ),General Well-Being Schedule (GWB),and Self-Reporting Questionnaire 20 (SRQ-20).Results There was significant difference among sub-groups with different educational backgrounds,different occupation,and different age group for total scores of perceived stress (F=5.026,5.637,6.421,P＜0.01).Female had significantly higher scores than male on total scores of negative coping factor of TCSQ,total scores SRQ-20 (26.33±6.29,4.36±3.84 vs 24.93±5.68,3.32±3.53) (all P＜0.05).Total scores of negative coping factor of TCSQ,total scores of positive coping factor of TCSQ,and total scores of perceived stress were the significant predictors of both total scores SRQ-20 and total scores of GWB (all P＜0.001).Both total scores of positive coping factor of TCSQ and total scores of negative coping factor of TCSQ mediated the effects of perceived stress on total scores of SRQ-20 (23.87％,29.33％,respectively) and total scores of GWB (24.29％,23.24％,respectively).Conclusion There do exist the mediating effects between coping strategies in perceived stress and mental health status,the improvement of coping modes of scientific and technical workers can be regarded as a useful intervention strategy to improve their mental health status.

Objective To observe the effect of comprehensive rehabilitation on breast cancer related lymphedema. Methods 70 patients with breast cancer related lymphedema were divided into experiment group (n=35) and control group (n=35). Both groups received intermittent pneumatic compression, while the experiment group received comprehensive rehabilitation including manual lymphatic drainage, muscle stretching, expanded the range of motion of shoulder, gradual resistance training, high frequency electrical therapy and health education. They were assessed with Disabilities of Arm, Shoulder and Hand Scale (DASH) and development of lymphedema before and 8 weeks after treatment. Results DASH scores and the development of lymphedema improved in both groups after treatment (P<0.05), and improved more in the experiment group than in the control group (P<0.05). Conclusion Comprehensive rehabilitation can further relieve breast cancer related lymphedema, and improve the upper extremity function.

Objective To investigate the effects of proprioceptive training and core stability training on the lower limbs motor function and balance in patients with hemiplegia after stoke. Methods 40 patients were randomly divided into treatment group (n=20) and control group (n=20). Both groups received routine rehabilitation and the treatment group received proprioceptive training and core stability training in addition. They were assessed with Fugl-Meyer Assessment (FMA) of lower limbs, Functional Ambulation Category (FAC), Berg Balance Scale (BBS), Timed Up-and-Go Test (TUGT) before and 6 weeks after treatment. Results The scores of FMA, FAC, BBS and TUGT improved in both groups after treatment (P<0.01), and improved more in the treatment group than in the control group (P<0.05). Conclusion Addition of proprioceptive training and core stability training may further improve the lower limbs motor function and balance of the hemiplegics after stroke.

Objective To isolate and identify the lung cancestem like cellfrom the cell line NCI-H1650 .MethodThe NCI-H1650 cellwere continuously cultured in serum-free medium with human insulin ,recombinanepidermal grow th facto(EGF) ,recombinanbasifibroblasgrowth facto(bFGF) and bovine serum albumin(BSA) ,and paclitaxel waadopted to induce the stem cell.Flow cytometry and immunofluorescenstaining were used to analyze the expression levelof lung cancestem cell markeCD133 and CD326 .The expressionof stem cell related markeOct4 ,Nanog and Sox-2 were detected by RT-Pcand West-ern blo.ResultThe NCI-H1650 cellcontinuously cultured by serum-free medium and induced by combining paclitaxel induction showed the spherical suspension growth .Compared with NCI-H1650 cellcultured by serum ,CD133 and CD326 had significantly high expression and the expression levelof Oct4 ,Nanog and Sox-2 were significantly increased (P＜0 .05) .Conclusion Adopting the serum-free culture in vitro combined with paclitaxel foinducing NCI-H1650 cellcan effectively isolate the stem cellamong them.

Objective To outline the diagnosis and treatment of a through-and-through urinary bladder to rectum-anal canal injury. Methods To review and summary 13 cases of through-and-through urinary bladder to rectum-anal canal injury.All patients were male and average age of 37 years old.Among them 4 cases reached hospital in 6 hours after injury,5 cases during 6～12 hours and 4 cases over 12 hours.Debridment and suture of urinary bladder to rectum-anal canal injure,effective revolution of urine and stools and full drainage in anterior sacrum space were performed.All of the 13 cases experienced operation of bladder exploration and drainage.1 among them underwent prostate gland cuting off because of the prostate gland injury.Another received the operation of urethra anastomoses.Both of them keep on catheterizing after operation.The management of anus and rectum hurt:3 cases received clearing and sewing up the wounnd of anus sphincter.8 cases received the repair of rectum passing through the anus.4 cases were not given remedy.7 patients received placing double thimble in the gap before sacrum.4 patients recieved placing eduction tube in the original wound.12 patients were given the operation of making sigmoid flexure fistula. Results All the 13 cases were cured.2 cases had postoperative infection in anterior sacrum space and were cured by full drainage.1 case was cured by secondary repairment after anal spincter repaired unsucessfully.1 case with rectovesical fistula was cured by conservative treatment.None suffered from dysuria,importence or anal stenosis.12 cases were visited and 1 was lost.Follow up was conducted from 10 months to 6 years.1 case with mild bowels incontinence recovered after training of lifting the anus for half a year.1 patient suffered from bladder calculi after a year and healed through lithotrity under cystoscope.The remaining 10 urinate and defecate normally,amonmg them 1 experienced urethra extends 6 times. Conclusions It was the key of improving treatment effect to master the clinical feature of a through-and -through urinary bladder to rectum-anal canal injury and diagnose correctly in the early stage and select reasonable operative procedure.

OBJECTIVE To study clinical application technique of skin soft tissue expansion in auricular reconstruction.METHODS 502 patients (517 ears)were selected for skin soft tissue expansion. Skin expander was implanted and liquid injected routinely to expand skin to obtain excessive skin for auricular reconstruction.RESULTS Of 502 patients (517 ears),10 patients(10 ears) had complicated skin hematoma formation which had been cleaned out successfully;12 patients(12 ears) had complicated skin expander uncover,skin expander was taken out and reposted 4 months later in 7 patients(7 ears) without affecting opertative effect;No infection was found and all patients obtain satisfactory effect. CONCLUSION Skillful and normative operation is the key for preventing complication.Fibre layer must be got rid of during operation.Skin expander auricular reconstruction has the advantage of obvious concave and protruding figure as well as satisfactory cubical sense.It is the perfect method for treating ear malformation at present.