Periodontal ligament may have independent response to orthodontic stimulation and thus initiate alveolar bone osteogenesis and osteoclasts. Orthodontic-induced alveolar bone remodeling has no bone loss,while inflammation can lead to alveolar bone loss,suggesting that force signal and inflammatory factors may induce the differentiation of undifferentiated cells in the periodontal ligament via different pathways. The strength of the force and the nature of the force (basal tension and fluid shear force) may affect the differentiation of periodontal ligament cells,and may produce antagonistic or synergistic effect with the inflammatory factors through complex autocrine and paracrine regulation,resulting in local bone reconstruction,which is manifested as bone deposition and bone absorption. Studies have shown that Wnt signaling is an important regulatory pathway for osteoblast differentiation. Inflammatory factors can block the differentiation of stem cells into osteoblasts. The Wnt pathway is closely related to the effects of force and inflammatory factors on the differentiation of periodontal ligament cells.

[Objective] To summarize the experience of Professor ZHANG Qin, a famous Chinese doctor in Zhejiang Province, in the treatment of dysmenorrhea by clinical syndrome differentiation. [Methods] Through the examples of teacher ZHANG's clinical treatment of primary dysmenorrhea and secondary dysmenorrhea in three cases, combined with the usual clinical experience of Professor ZHANG Qin to follow, analyze and summarize teacher ZHANG's clinical experience of the treatment of dysmenorrhea. [Results] Three cases show that, whether it is the primary dysmenorrhea or secondary dysmenorrhea, ZHANG believes that the total incidence of cold stagnation of Qi, blood circulation is not smooth, the main reason for the lack of collateral. A sense of cold dampness to menstrual wading, passengers in the cytoplasm, with warm and cold, blood, Tiaochong;with blood stasis, expelling, warming channels to dispel cold, removing blood stasis and relieving pain; also, liver blood deficiency and blood stasis of uterus, cure properly via removing film, streteving liver and removing stasis to relieve pain. Along with symptoms, with mild blood for treatment, in order to reconcile Qi and blood, Chongren unblocked. [Conclusion] Professor ZHANG Qin's experience in the treatment of dysmenorrhea by clinical syndrome differentiation has distinctive features and positive effects, so it is worth in-depth study and application.

Objective:Our aim was to evaluate the relationship of E-cadherin（EC） expression with the biological behavior of lung cancer.Methods:The EC expression and the cellular DNA ploidy were measured using flow cytometry and immunofluorescence methods in 39 cases of primary non-small cell lung cancer. Systemic pathological examination were performed for all cases.Results:The EC expression significantly reduced in all cases of lung cancer.Fluorescene index(FI) of EC expression was (0.90±0.10) in normal lung tissue， (0.49±0.09) in squamous cell lung cancer,(0.63±0.08) in adenocarcinoma.The expression of EC in squamous lung cancer greatly decreased in poor differentiation and with lymph node metastasis (P<0.01).In squamous cell lung cancer,the expression of EC was lower in uneuploid cancer than that in diploid cancer (P<0.05).The expression of E-Cadherin in squamous cell lung cancer reduced more in TNM Ⅲ than in TNM Ⅰ,Ⅱ.Conclusion:The expression of EC decreased in poor differentiation squamous cell lung cancer with lymph node metastasis. In lung adenoma, the decrease in the expression of EC has no relatoin with the TNM stage, lymph node metastasis, and DNA ploidy.

Designed experiment can improve the students'study interest and train their scientific research ability in bioanalytical chemistry experiment teaching.This article probes into the devise of designed experiment,the selectivity of the contents and the detailed process of the experiments.

Objective To explore the influence of some factors on long-term survival of postoperative stage Ⅰ NSCLC patients. Methods 91 patients of NSCLC who underwent radical surgery of the primary tumor with dissection of the hilar and mediastinal lymph nodes were diagnosed as stage Ⅰ NSCLC postoperatively by pathology and followed up for 5 years. Its hilar and subcarinal lymph nodes were detected occult micrometastastic tumor cells by immunohistochemistry (SP method) by using the binoclonal antibody multicytokeratin (AE1/AE3) as a micrometastatic marker. To analyse the influence of micrometastasis and the clinicopathologic characteristics on long-term survivals. Results The rate of micromatastasis of stage Ⅰ NSCLC was 49 %. The five-year overall survival rate was 70.3 %. The median of survival time was 48.5months. The rate of metastasis was 32 % and the meantime of relapse and metastasis was 36.6months. Tumor size, differentiation, stage, and micrometastasis were significantly associated with relapse and metastasis (P <0.05). The tumor differentiation, stage, and micrometastasis were found to be significant independent factor on survival in multivariate analysis (P<0.05). Conclusion There was nodal micrometastasis in completely resected stage Ⅰ NSCLC, and the tumor differentiation, stage, and micrometastasis were found to be significant independent factor on survival.

Carcinoma, Hepatocellular ; diagnosis ; therapy ; Gastroenterology ; trends ; Hepatitis, Viral, Human ; diagnosis ; therapy ; Humans ; Liver Cirrhosis ; diagnosis ; therapy ; Liver Diseases ; diagnosis ; therapy ; Liver Failure, Acute ; etiology ; therapy ; Liver Neoplasms ; diagnosis ; therapy ; Retrospective Studies

Interferons ; Liver Diseases

Humans ; Liver Cirrhosis ; therapy

In two models for DNA replication and transcription, tra ditional one known as sliding model postulates that proteins involving replicati on and transcription track on the DNA template as a locomotive. In factory model proposed recently, those proteins are immobilized on nuclear structure, to pull the template. Growing evidence from biochemistry, biophysics, and cell biology suggests that the factory model is an actual fact in vivo.

Long-term outcomes of surgical bile duct injury (BDI) repair are affected by multiple factors, such as surgeon's experience, severity of BDI, pathological changes of the proximal duct, and the manner and timing of surgical repair. However, the timing of BDI repair is still controversial still. In this article, the authors reviewed the correlation of pathophysiologic changes of the proximal duct, repair timing and outcome discussed in English references. It was shown that pathophysiologic changes of the proximal duct are a major contributor to the timing of BDI repair.