Objective:To understand the iodine nutrition status of pregnant women in Huzhou City, and to provide a basis for timely targeted prevention and control of the iodine deficiency disorders.Methods:In 2017, five districts (counties) in Wuxing, Nanxun, Deqing, Changxing, and Anji were used as monitoring sites in Huzhou City, and each monitoring site was divided into five areas according to the east, west, south, north, and middle locations. One township (street) in each location was sampled to collect drinking water samples from residents, and the water iodine content was tested; 21 pregnant women (7 each in the early, middle, and late pregnancy periods) were sampled in each township (street), and edible salt samples were collected at home for testing salt iodine and urine samples were collected for testing urinary iodine content.Results:A total of 332 drinking water samples were collected from Huzhou residents, water iodine median was 2.2 μg/L, which belonged to iodine-deficient regions; and there was statistically significant difference in the water iodine median between different regions ( H = 161.0, P < 0.05). And 525 edible salt samples were detected from pregnant women, the salt iodine median was 23.4 mg/kg, the salt iodine coverage was 97.1% (510/525), and the qualified iodized salt consumption rate was 93.0% (488/525). There was a statistically significant difference in salt iodine levels between different regions ( H = 67.7, P < 0.05). Meanwhile, the urinary iodine median of 525 urine samples of pregnant women was 123.1 μg/L, which was at the level of iodine deficiency. From the regional distribution, the urinary iodine median in Deqing County was 154.0 μg/L, which was the highest and at an iodine appropriate level; the other regions were Wuxing District, Nanxun District, Anji County, and Changxing County in order, all of them were at the iodine deficiency level; the urinary iodine median of pregnant women between different regions was statistically significantly different ( H = 14.1, P < 0.05). From the pregnancy periods' distribution, the urinary iodine median in middle pregnancy period was the highest at 172.8 μg/L; there was statistically significant difference between different pregnancy periods ( H = 7.5, P < 0.05). Conclusions:The Huzhou City belongs to the environmental iodine deficiency area. Except for Deqing County, the iodine nutrition status of pregnant women in other districts (counties) is at an iodine deficiency level. Urinary iodine level monitoring and health education of pregnant women should be strengthened to improve iodine nutrition status.