1. A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica
Pervaiz IQBAL ; Dildar AHMED ; Muhammad Nadeem ASGHAR
Asian Pacific Journal of Tropical Medicine 2014;7(S1):S473-S480
Objective: To evaluate antioxidant and radical scavenging activities of organic extracts from fruit, roots and aerial parts of Fagonia cretica. Methods: Shed dried and powdered plant parts were initially extracted in methanol and subsequently partitioned in n-hexane, chloroform, ethyl acetate and 1-butanol successively. Antioxidant and radical scavenging potential of the methanol extracts and the fractions of each part were evaluated using total phenolic contents (TPC) and total flavonoid contents (TFC), 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation radicals scavenging, reducing power (potassium ferricyanide-trichloroacetic acid system), ferric ion reducing antioxidant potential, lipid peroxidation inhibition activity (linoleic acid system) and total antioxidant activity (phosphomolybdate) assays. Results: TPC and TFC values for methanol extracts and various fractions ranged from 0.23-4.30 mg/L gallic acid equivalents and from 30-545 mg/L rutin equivalents, respectively. Overall, methanol extracts and all the fractions of root and aerial parts showed higher TPC and TFC values. Methanol extracts and aqueous fractions of root and aerial parts and the n-butanol fraction of root showed lower EC
2. Phenolic and flavonoid contents and anti-oxidative potential of epicarp and mesocarp of Lagenaria siceraria fruit: A comparative study
Dildar AHMED ; Munim FATIMA ; Sana SAEED
Asian Pacific Journal of Tropical Medicine 2014;7(S1):S249-S255
Objective: To conduct a comparative analysis of the phenolic and flavonoid contents and anti-oxidative potential of epicarp and mesocarp of Lagenaria siceraria fruit. Methods: The dried methanolic extracts of mesocarp and epicarp of the fruit and their hexane, chloroform, ethyl acetate, n-butanolic and aqueous fractions were subjected to antioxidant assays including ferric reducing antioxidant potential, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, reducing power capacity, 2,2-diphenyl-1-picrylhydrazyl, lipid peroxidation inhibitory and phosphomolybdate assays. Total phenolic and flavonoid contents were also determined. Results: Ethyl acetate fractions of epicarp and mesocarp had considerable amounts of phenolics (243.50 and 109.50 μg/mL of gallic acid equivalents, respectively). 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of ethyl acetate fractions of both the plant parts showed higher activity than vitamin C with IC
3. Lipase inhibitory activity of Lagenaria siceraria fruit as a strategy to treat obesity
Maria MAQSOOD ; Dildar AHMED ; Iqra ATIQUE ; Wajeeha MALIK
Asian Pacific Journal of Tropical Medicine 2017;10(3):305-310
Objective To explore pancreatic lipase inhibitory activity under different extraction conditions in order to track the most potent extract. Methods The methanolic extract and its fractions in solvents of increasing polarity, ether, chloroform, ethyl acetate, n-butanol and water, were made through cold maceration. Extracts in ethanol, ethyl acetate, acetone and chloroform were similarly prepared. Aqueous extract was prepared through hot decoction method. A reported method was used to determine lipase inhibitory activity of extracts and fractions over wide ranges of concentrations. Results The extracts and fractions exhibited concentration dependent activity. The IC
4. Antimicrobial activities of methanolic extract of Carissa opaca roots and its fractions and compounds isolated from the most active ethyl acetate fraction
Dildar AHMED ; Ramsha SAEED ; Nasir SHAKEEL ; Khaizran FATIMA ; Aneela ARSHAD
Asian Pacific Journal of Tropical Biomedicine 2015;5(7):541-545
Objective: To study the antibacterial and antifungal activities of methanolic extract of roots of Carissa opaca and its fractions in hexane, chloroform, ethyl acetate, n-butanol and water, and the isolated compounds. Methods: The zones of inhibition of the samples against test microorganisms were determined by agar well diffusion method. Minimum inhibitory concentrations of the samples were determined by agar well dilution method. Test microorganisms included four standard bacteria [Bacillus subtilis ATCC 6633 (B. subtilis), Escherichia coli ATCC 8739 (E. coli), Pseudomonas aeruginosa ATCC 9027 (P. aeruginosa), and Staphylococcus aureus ATCC 6538], two standard fungi [Candida albicans ATCC 10231 (C. albicans)] and Aspergillus niger, and six clinical isolates (B. subtilis, E. coli, P. aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterobacter cloacae). The most active fraction was investigated to isolate compounds. The chemical compounds isolated from the ethyl acetate fraction were identified by gas chromatography-mass spectrometer, high performance liquid chromatography and liquid chromatographymass spectrometer. Results: E. coli, P. aeruginosa, and C. albicans were the most susceptible. Less polar fractions exhibited stronger efficacy than polar ones, and ethyl acetate fraction proved to be the most potent. Zones of inhibition of hexane, chloroform and ethyl acetate fractions, and amoxil against C. albicans were 19.96, 22.01, 23.10 and 19.20 mm, respectively. Ethyl acetate faction was the most toxic to all the test microorganisms, with minimum inhibitory concentrations of 8.0, 7.8 and 7.78 μg/mL against P. aeruginosa, C. albicans and B. subtilis, respectively. Isolated compounds, limonene, 2'-hydroxyacetophenone, vanillin, naphthalenone, 2,3,3-trimethyl-2-(3-methylbuta-1,3-dienyl)-6-methylenecyclohexanone, 2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester, β-sitosterol, vitamin E, rutin, quercetin, lupeol, epigallocatechin, showed considerable antimicrobial activities against test microorganisms. Conclusions: The roots of Carissa opaca contain compounds with significant antimicrobial potential.