A study of changes in testosterone(T) and dihydrotestosterone(DHT) serum concentration was undertaken in children from 6 months to 15 year old age to elucidate the differences in concentration of T and DHT in pediatric age. In addition, change in T: DHT ratio was studied in all children. In same age group there was large individual variation even though they were considered to be normal in T and DHT levels. The results were 1. The T concentration in blood did not increase until the age of l2 year old, but sharply increase(7-8 times) and sustained from the age of 12 year old. 2. DHT showed constant level until the age of 6 year old and somewhat increased level (1.5 times) from the 7 year old age until 11 year old age. So total serumT was considered to be increased a little and converted to DHT promptly from the age of 7. 3.Seeing the T : DHT ratio at the beginning of puberty, the conversion of T to DHTwas considered to be slow in activation time than the T production.
Adolescent ; Child ; Dihydrotestosterone* ; Humans ; Puberty ; Testosterone*

A study of changes in testosterone(T) and dihydrotestosterone(DHT) serum concentration was undertaken in children from 6 months to 15 year old age to elucidate the differences in concentration of T and DHT in pediatric age. In addition, change in T: DHT ratio was studied in all children. In same age group there was large individual variation even though they were considered to be normal in T and DHT levels. The results were 1. The T concentration in blood did not increase until the age of l2 year old, but sharply increase(7-8 times) and sustained from the age of 12 year old. 2. DHT showed constant level until the age of 6 year old and somewhat increased level (1.5 times) from the 7 year old age until 11 year old age. So total serumT was considered to be increased a little and converted to DHT promptly from the age of 7. 3.Seeing the T : DHT ratio at the beginning of puberty, the conversion of T to DHTwas considered to be slow in activation time than the T production.
Adolescent ; Child ; Dihydrotestosterone* ; Humans ; Puberty ; Testosterone*

Androgen treatment in boys with underdeveloped external genitalia requires close attention to avoid the side effects. The dose and duration of androgen have usually been decided according to the validation of the effects and the side effects. However, the dosage that showed the desirable effect or undesirable side effects already exceeded the optimal dosage. So the indicator to predict the effect of androgen is necessary for boys during androgen treatment. To evaluate serum 5alpha-androstane-3alpha, 17beta-diol glucuronide as this indicator we tried to obtain the correlation between the increase of penile length and serum 5alpha-androstane-3alpha, 17beta-diol-glucuronide and dihydro-testosterone during dihydrotestosterone treatment. While the mean serum level of 5alpha-androstane-3 alpha,17beta-diol glucuronide was higher than that of dihydrotestosterone in 17 patients without defects of androgen action,the opposite result was observed in 6 patients with defect of androgen action. The increase in penile length estimated by the calculation of the 8 weeks difference of deviation/normal S.D.significantly correlated with the serum 5alpha-androstane-3alpha, 17beta-diol glucuronide but not with serum DHT. Therefore the responsibility of the target cell to androgen seems to determine both the effect of androgen treatment and the serum level of 5alpha-androstane-3alpha, 17beta-diol glucuronide. In conclusion the serum 5alpha-androstane3alpha, 17beta-diol glucuronide could have a value as the indicator to predict the effect of androgen treatment in children with underdeveloped external genitalia.
Child* ; Dihydrotestosterone ; Genitalia ; Humans ; Male ; Penis

Based on the theory that benign prostatic hypertrophy may be induced by androgenic effect of testosterone derivatives, especially 5-alpha - dihydrotestosterone, on prostatic tissue, Chlormadinone acetate(CMA), potent oral synthetic antiandrogen was investigated in the treatment of benign prostatic hypertrophy. Twenty-two patients of prostatic hypertrophy were studied over six months period with a special reference to uroflowmetry and following results were obtained : 1) Chlormadinone acetate induced improvement of obstructive urinary symptoms in terms of uroflowmetric measurement. 2) It is very worthwhile to initiate medical treatment before undergoing any surgical intervention or when surgery is contraindicated.
Chlormadinone Acetate* ; Dihydrotestosterone ; Humans ; Prostatic Hyperplasia* ; Testosterone

Finasteride, an inhibitor of type II 5alpha-reductase, inhibits conversion of testosterone to dihydrotestosterone (DHT), resulting in a decrease in serum and scalp DHT levels which are believed to be pathogenic in androgenic alopecia. Oral finasteride has been shown to be effective in the treatment of hair loss in men, while its efficacy in women has remained controversial. Herein, we report 10 cases of female patterned hair loss in postmenopausal women treated with 1 mg/day of finasteride.
Alopecia ; Dihydrotestosterone ; Female* ; Finasteride* ; Hair* ; Humans ; Male ; Scalp ; Testosterone

The mechanism by which hypospadias is induced has not yet been clearly clarified. However insufficiency of the hypothalamo-pituitary-gonadal function has been suggested in the hypospadias while conflicting results have been demonstrated. Therefore, we assessed pituitary-gonadal function of hypospadias by measuring serum concentrations of testosterone, dihydrotestosterone and T:DHT ratio before and after HCG treatment. Pituitary LH and FSH responses to exogenous LHRH were also measured. The results were as follows ; 1. There were no significant differences in basal concentrations of serum testosterone and DHT between control and hypospadias. T:DHT ratio was not significantly different between two groups. 2. Concentrations of serum testosterone, DHT, LH and FSH were significantly elevated after HCG of LHRH administration except two patients with familial hypospadias. Also, T:DHT ratio showed normal level in hypospadias group after HCG stimulation. There results suggest that hypospadias may not be associated with abnormality in pituitary-gonadal function except familial hypospadias.
Dihydrotestosterone ; Female ; Gonadotropin-Releasing Hormone ; Gonads ; Humans ; Hypospadias* ; Male ; Testosterone

PURPOSE: The aim of this study was to investigate the anti-inflammatory effects of a new herbal formula (WSY-1075) in a nonbacterial prostatitis rat model. MATERIALS AND METHODS: Prostatitis was induced in male Wistar rats (n=32) by treatment with 17 beta-estradiol and dihydrotestosterone for 4 weeks. After the induction of prostatitis, the rats were randomly divided into one of four treatment groups: control (n=8), ciprofloxacin (n=8), WSY-1075 (100 mg/kg) (n=8), and WSY-1075 (400 mg/kg) (n=8). After 4 weeks of treatment, the prostatic proinflammatory cytokine (tumor necrosis factor-alpha, interleukin [IL]-6, and IL-8) levels and histological findings were noted. RESULTS: The ciprofloxacin and WSY-1075 treatment groups showed significantly decreased proinflammatory cytokine levels compared with the control group. Histologically, treatment with ciprofloxacin and WSY-1075 significantly suppressed the severity of prostatitis lesions compared with those in the control group. No differences in the proinflammatory cytokine levels or histologic findings were observed with the dose dependent treatment of WSY-1075. CONCLUSIONS: The new herbal formula, WSY-1075, showed effective anti-inflammatory activities in the prostate and may be useful for the clinical treatment of nonbacterial prostatitis. Our findings suggest that WSY-1075 has a beneficial effect on the prevention and treatment of nonbacterial prostatitis.
Animals ; Ciprofloxacin ; Dihydrotestosterone ; Estradiol ; Humans ; Inflammation ; Interleukins ; Male ; Prostate ; Prostatitis ; Rats ; Rats, Wistar

Prostate cancer is the most frequently diagnosed cancer. Although prostate tumors respond to androgen ablation therapy at an early stage, they often acquire the potential of androgen-independent growth. Elevated transcriptional activity of androgen receptor (AR) and/or signal transducer and activator of transcription-3 (STAT3) contributes to the proliferation of prostate cancer cells. In the present study, we examined the effect of resveratrol, a phytoalexin present in grapes, on the reporter gene activity of AR and STAT3 in human prostate cancer (LNCaP-FGC) cells stimulated with interleukin-6 (IL-6) and/or dihydrotestosterone (DHT). Our study revealed that resveratrol suppressed the growth of LNCaP-FGC cells in a time- and concentration-dependent manner. Whereas the AR transcriptional activity was induced by treatment with either IL-6 or DHT, the STAT3 transcriptional activity was induced only by treatment with IL-6 but not with DHT. Resveratrol significantly attenuated IL-6-induced STAT3 transcriptional activity, and DHT- or IL-6-induced AR transcriptional activity. Treatment of cells with DHT plus IL-6 significantly increased the AR transcriptional activity as compared to DHT or IL-6 treatment alone and resveratrol markedly diminished DHT plus IL-6-induced AR transcriptional activity. Furthermore, the production of prostate-specific antigen (PSA) was decreased by resveratrol in the DHT-, IL-6- or DHT plus IL-6-treated LNCaP-FGC cells. Taken together, the inhibitory effects of resveratrol on IL-6- and/or DHT-induced AR transcriptional activity in LNCaP prostate cancer cells are partly mediated through the suppression of STAT3 reporter gene activity, suggesting that resveratrol may be a promising therapeutic choice for the treatment of prostate cancer.
Dihydrotestosterone ; Genes, Reporter ; Humans ; Interleukin-6 ; Prostate ; Prostate-Specific Antigen ; Prostatic Neoplasms* ; Receptors, Androgen ; Transducers ; Vitis

BACKGROUND: Although it is evident that androgens have an important role in pathogenesis of acne, the results of current studies on the relationship between serum androgens and acne are inconsistent. Additionally there has been sparse clinical datas about hormonal derangcment with regard to acne in Korea. OBJECTIVE: This study was conducted to elucidate hormonal abnormalities by detecting the quantitative difference between the acne patients and normal persons with respect to the levels of plasma free/total Testosterone(T), Dihydrotestosterone(DHT), Dehydroepiandrosterone-sulfate(DHEA-S) and Estradiol(E2) in acne patients. METHODS: In this study, we measured serum levels of free T, total T, DHT, DHEA-S and E2 in 25 women and in 15 men with acne and in 40 healthy control subjects. RESULTS: 1. The levels of free T and E2 in the male patient group were significantly higher than those in the control groups. In the female patient group the levels of DHT were more elevated than those in the control groups. 2. The levels of free T and E2 in all the male subgroups classified by Burke & Cunliffe were significantly elevated compared to those in the male control groups. The plasma levels of DHT in all the female clinical subgroups were significantly higher than those in the female control groups. However, we could not find any correlative relationship between hormonal parameters and the clinical severity in all the patient groups. 3. There was a positive relationship between free T levels and E2 levels in the male patient gfOUp. 4. The patients with menstrual cyclic irregularity had higher mean free T and acne grading scores when compared with those who had regular periods. Also they were younger than the patients with regular menstrual cyclicity. CONCLUSION: The levels of free T and E2 in male patients were higber than in normal control subjects. In the female patients the levels of DHT were higher than in the control subjects. However, there was no correlative relationship between hormonal parameters and the clinical severity of acne. Also the patients with irregular menstrual cyclicity had a higher free T and showed severe acne clinically.
Acne Vulgaris* ; Androgens ; Dihydrotestosterone* ; Estradiol* ; Female ; Humans ; Korea ; Male ; Periodicity ; Plasma* ; Testosterone*

The gubernaculum testis, which has an important role to play in transinguinal testicular descent is richly supplied by the genitofemoral nerve through its scrotal attachment. The treatment of pregnant rats with antiandrogen disturbed normal testicular descent in their male offspring. The division of the genitofemoral nerve in neonatal rats also disturbed normal descent of testis. The spinal motonucleus of genitofemoral nerve showed sexual difference. Therefore the genitofemoral motonucleus might be altered morphologically by manipulation of neonatal androgen as the other motonuclei showing sexual difference. This experiment was designed to illustrate the morphological relationship between the androgens and the genitofemoral motonucleus by retrograde tracing method in rats. Preliminarily the genitofemoral motonucleus of normal male and female adults were compared. The effects of neonatal androgen on genitofemoral rnotonucleus were evaluated by observing the motonuclei of adults after bilateral orchiectomy in neonates and androgen compensation. The results are summarized as follows. 1. The genitofemoral motonuclei of males were similar to those of females in location, but the number and the size of their cells were larger than those of females. 2. The bilateral orchiectomy in neonates caused atrophy of the cremasteric muscle and decreased the size and the number of genitofemoral motonucleus cells in adults. 3. The neonatal compensation of the androgen after bilateral orchiectomy could not prevent atrophy of the cremaster muscle, but maintained the number and the size of the genitofemoral motonucleus cells in adult compared to control. 4. The compensatory effects of androgens on the cellular size of genitofemoral motonucleus were obvious in dihydrotestosterone rather than testosterone. From the above results, it is concluded that the genitofemoral motonucleus show sexual difference and is morphologically affected by neonatal androgens. Therefore there could be the process that androgens, especially dihydrotestosterone, affects genitofemoral motonucleus which affects testicular descent in rats.
Adult ; Androgens* ; Animals ; Atrophy ; Compensation and Redress ; Dihydrotestosterone ; Female ; Humans ; Infant, Newborn ; Male ; Orchiectomy ; Rats ; Testis ; Testosterone