1.Effect of different carbon sources on pyruvic acid production by using lpdA gene knockout Escherichia coli.
Dongqian SHEN ; Xiaoyu FENG ; Dongqiang LIN ; Shanjing YAO
Chinese Journal of Biotechnology 2009;25(9):1345-1351
We studied the ability of lpdA gene knockout Escherichia coli to ferment different sugars in mineral salts medium for the production of pyruvate. The sugars studied were glucose, fructose, xylose and mannose at a concentration of 10 g/L. At the same time, effect of inoculum size on lpdA fermentation with glucose was studied. The strain was able to use all sugars for biomass generation and pyruvate production. The lpdA knockout mutant converted glucose, fructose, xylose and mannose to pyruvate with yields of 0.884 g/g, 0.802 g/g, 0.817 g/g and 0.808 g/L, respectively. The pyruvate accumulation curve coupled with cell growth except for mannose as carbon source. When the inoculation size increased, the rate of glucose consumption, pyruvate accumulation and cell growth increased but lower pyruvate concentration. This study demonstrates that E. coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.
Carbon
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metabolism
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Dihydrolipoamide Dehydrogenase
;
genetics
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Escherichia coli
;
genetics
;
metabolism
;
Escherichia coli Proteins
;
genetics
;
Fermentation
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Fructose
;
metabolism
;
Gene Knockout Techniques
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Glucose
;
metabolism
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Mannose
;
metabolism
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Pyruvic Acid
;
metabolism
;
Xylose
;
metabolism
2.Clinical and genetic analysis of a case of dihydrolipoamide dehydrogenase deficiency caused by novel variant of DLD gene.
Shengnan WU ; Yongxing CHEN ; Qiong CHEN ; Linghua SHEN ; Haiyan WEI
Chinese Journal of Medical Genetics 2020;37(12):1356-1359
OBJECTIVE:
To analyze the clinical and genetic characteristics of a patient with dihydrolipoamide dehydrogenase deficiency.
METHODS:
Potential variants of the DLD gene were detected by whole exome sequencing and verified by Sanger sequencing.
RESULTS:
Compound heterozygous variants, c.704_705delTT (p.Leu235Argfs*8) and c.1058T>C (p.Ile353Thr), were detected in the DLD gene. The c.1058T>C (p.Ile353Thr) variant was derived from his mother and known to be pathogenic. The c.704_705delTT (p.Leu235Argfs*8) variant was derived from his father and was unreported previously.
CONCLUSION
The compound heterozygous variants of c.704_705delTT (p.Leu235Argfs*8) and c.1058T>C (p.Ile353Thr) of the DLD gene probably underlay the disease in this patient. Above finding has facilitated genetic counseling and prenatal diagnosis for the family.
Acidosis, Lactic/genetics*
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Dihydrolipoamide Dehydrogenase/genetics*
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Female
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Genetic Testing
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Genetic Variation
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Humans
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Male
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Maple Syrup Urine Disease/genetics*
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Pregnancy
;
Whole Exome Sequencing
3.Expressions of c-Fos and NADPH-d in the related brainstem during vestibular compensation.
Acta Academiae Medicinae Sinicae 2008;30(6):655-658
OBJECTIVETo study the mechanism of vestibular compensation and to observe the changes of c-Fos and NADPH-d expressions in the brainstem of the vestibular deafferentation rats in static status or following angular acceleration stimulation.
METHODSTotally 60 SD rats were randomly divided into control group (labyrinthine intact), complete unilateral vestibular deafferentation (UVD) group, simultaneous complete bilateral vestibular deafferentation (BVD) group (n = 20 in each group). Subgroups (n = 10 in each subgroup) were set for static status or following angular acceleration stimulation in each group. Double labeling with histochemistry-immunohistochemistry was performed to observe c-Fos/NADPH-d neurons.
RESULTSNo positive c-Fos/NADPH-d expression was observed in the both sides of medial vestibular nucleus (MVN) and prepositus hypoglossi (PrH) of normal rats in static status and BVD rats whether following canal rotation or not. c-Fos/ NADPH-d expression was observed in the ipsilesional MVN and the contralesional PrH of UVD rats. However, c-Fos/NADPH-d were detected in both sides of MVN and PrH in UVD rats and normal rats following angular acceleration stimulation.
CONCLUSIONIn the ipsilesional MVN and the contralesional PrH, c-Fos plays an important role in vestibular compensation, in which nitric oxide acts as a key neurotransmitter.
Animals ; Brain Stem ; metabolism ; Dihydrolipoamide Dehydrogenase ; genetics ; metabolism ; Female ; Gene Expression ; Male ; Proto-Oncogene Proteins c-fos ; genetics ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Vestibule, Labyrinth ; physiology