OBJECTIVE: A novel technology for detection of diatom was discussed. METHODS: Five grams of testing sample were taken and the organics were removed using simple mechanical knead pulp method. The homogenized samples were concentrated by centrifugation, smeared, and then examined under light microscope. RESULTS: Except for a few feather's grains, the vast majority of diatom could be identified easily with clear diatom striations. The organic diatom could also be easily detected by this methodology. CONCLUSION The detection of diatom using knead pulp method is not only simple and inexpensive with a higher successful rate, but also causes nearly no harm to human and environment.
Calcium/metabolism* ; Diatoms/ultrastructure* ; Drowning/diagnosis* ; Forensic Pathology ; Humans ; Kidney ; Liver ; Lung ; Microscopy/methods* ; Specimen Handling/methods* ; Tooth