3.Molecular epidemiology of rotavirus in children with acute diarrhea in Shanghai port area.
Yu-xia ZHANG ; Qi-rong ZHU ; Mei ZENG
Chinese Journal of Epidemiology 2010;31(1):103-104
Child
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Child, Preschool
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China
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epidemiology
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Diarrhea
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epidemiology
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virology
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Humans
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Infant
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Molecular Epidemiology
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Rotavirus
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genetics
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Rotavirus Infections
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epidemiology
;
virology
4.Etiological study on viral diarrhea among children in Lulong, Hebei province.
Dan-di LI ; Qiu-li YU ; Shun-xiang QI ; Yun XIE ; Zhao-jun DUAN
Chinese Journal of Epidemiology 2010;31(3):350-351
Child, Preschool
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China
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epidemiology
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Diarrhea
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epidemiology
;
etiology
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virology
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Humans
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Infant
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Rotavirus
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Rotavirus Infections
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epidemiology
5.Pathogens of high incidence of other infectious diarrhea in Guangdong Province, from November 2008 to January 2009.
Dan-Di LI ; Hui LI ; Ru-Ning GUO ; Wei-Xia CHENG ; Na LIU ; Jie-Mei YU ; Bei LAN ; Jin-Xin XIE ; Zhao-Jun DUAN
Chinese Journal of Virology 2010;26(2):150-152
From November 2008 to January 2009, a sharp increase of diarrhea in children in Guangdong province appeared, we randomly collected 53 stool specimens from out-patient children with dirrhea in 3 major hospitals (Guangzhou City Children's Hospital, Shenzhen Baoan District Maternal and Child Health Hospital, First Affiliated Hospital of Shantou University). Rotavirus and calicivirus were screened by ELISA and RT-PCR. We found 29 cases of rotavirus infection with diverse serotypes. Only four cases were identified as calicivirus infection. The result indicated that rotavirus was a major pathogen of this high incidence of diarrhea from November 2008 to January 2009 in Guangdong Province.
Age Distribution
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Child, Preschool
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China
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epidemiology
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Diarrhea
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diagnosis
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epidemiology
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virology
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Diarrhea, Infantile
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diagnosis
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epidemiology
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virology
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Feces
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virology
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Humans
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Incidence
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Infant
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Reverse Transcriptase Polymerase Chain Reaction
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Rotavirus
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classification
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genetics
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isolation & purification
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Rotavirus Infections
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diagnosis
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epidemiology
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virology
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Serotyping
6.Molecular epidemiological study of adult rotavirus infection diarrhea in Wuhan City.
Yuan-hong WANG ; Xuan ZHOU ; Jian-guo SHAN ; Dun-jin ZHOU ; Ping DIAO ; Yan LI ; Yun ZHAO ; Cong-rong LI
Chinese Journal of Epidemiology 2004;25(9):818-818
Adolescent
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Adult
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Aged
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China
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epidemiology
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Diarrhea
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epidemiology
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virology
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Female
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Humans
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Male
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Molecular Epidemiology
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Rotavirus
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genetics
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Rotavirus Infections
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epidemiology
;
genetics
7.The molecular epidemiology characteristics of norovirus in environment and clinical samples in Guangzhou from 2009 to 2011.
Lan LUO ; Xin-wei WU ; Yu-fei LIU ; Qiao-yan LI ; Hua-ping XIE ; Ye-jian WU ; Lei LI ; Li-yun JIANG ; Xia YANG
Chinese Journal of Preventive Medicine 2013;47(1):40-43
OBJECTIVETo investigate the molecular epidemiological characteristics of norovirus in Guangzhou from 2009 to 2011.
METHODSA total of 183 water samples, 1162 seafood samples and 1066 diarrhea stool specimens were collected from January 2010 to May 2011, June 2009 to June 2011 and July 2009 to December 2010 respectively in Guangzhou. Norovirus was detected by real time reverse transcript-PCR (qRT-PCR). The partial polymerase gene was amplified from norovirus positive samples, then sequenced and compared with the sequences of norovirus in GenBank. The phylogenetic tree was created.
RESULTSThe positive rate was 19.67% (36/183), 8.26% (96/1162) and 37.05% (395/1066) in water samples, seafood and diarrhea patients respectively. Noroviruses from positive samples could be divided into 10 representative strains, in which 7 representative strains of genotype of 208 samples was type G2-4. The sequences from water, seafood and stool specimens were highly homologous with the similarity of 94% - 100%.
CONCLUSIONIn Guangzhou, the predominant Norovirus genotype was G2-4 and the positive rate of samples was high.
Base Sequence ; Caliciviridae Infections ; epidemiology ; virology ; China ; epidemiology ; Diarrhea ; virology ; Genotype ; Humans ; Molecular Epidemiology ; Norovirus ; classification ; genetics ; isolation & purification ; Phylogeny ; RNA, Viral ; genetics ; Seafood ; virology ; Water Microbiology
8.Etiological study of human bocavirus 1-4 in children with acute diarrhea in Lanzhou, China.
Jing-Yao XIANG ; Dan-Di LI ; Xin MA ; Yan-Qing GUO ; Zhao-Jun DUAN ; Yu-Ning LI
Chinese Journal of Virology 2014;30(4):402-407
This study aimed to study the epidemiological and clinical characteristics of human bocavirus 1-4 (HBoV1-4) in children with acute diarrhea in Lanzhou and to investigate the association between HBoV and acute gastroenteritis. A total of 331 stool samples were collected from children aged under 5 years with acute diarrhea at the Department of Pediatrics, the First Hospital, Lanzhou University, between July 2012 and June 2013. Nested PCR was used to screen for HBoV and a general PCR was employed to screen other common diarrhea viruses. We found human bocavirus 1, 2, 3 and 4 in 26, 15, 7 and 1 cases, respectively. There was no specific seasonal distribution of HBoV, with infections occurring throughout the year. HBoV was mostly found in children aged between 7 and 12 months, with a mean age of 11.04 months (+/- 6.92 months), and 93.88% of affected children were aged under 2 years. Overall, 71.3% of mixed infections were mixed and the majority of other infections were caused by rotavirus. There was no statistical difference in the incidence of fever and vomiting associated with HBoV infection. A rare virus strain, HBoV4 (LZFB086), was identified, which showed highest levels of nucleotide sequence identity (99.0%) with a single Thai HBoV strain (JQ267789). No case of HBoV2B was found. In conclusion, HBoV1 was a major etiological pathogen of HBoV in pediatric cases in Lanzhou. HBoV4 was detected in feces for the first time in China. The rate of mixed infections was high and rotavirus was dominant. The data presented suggests that HBoV is not a major causative agent of gastroenteritis.
China
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epidemiology
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Diarrhea
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epidemiology
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virology
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Feces
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virology
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Human bocavirus
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classification
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genetics
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isolation & purification
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Humans
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Infant
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Molecular Sequence Data
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Parvoviridae Infections
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epidemiology
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virology
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Phylogeny
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Seasons
9.Rotavirus diarrhea among children in three hospitals under sentinel surveillance, from August 2001 to July 2004.
Li-jie ZHANG ; Zhao-yin FANG ; Li-wei SUN ; Du ZENG-QING ; Jing-yu TANG ; Guang ZENG ; Duncan STEELE ; Paul KILGORE ; Xi JIANG ; Roger GLASS ; Bao-ming JIANG
Chinese Journal of Epidemiology 2007;28(5):473-476
OBJECTIVETo learn the epidemiologic characteristics of rotavirus diarrhea in three hospitals under sentinel surveillance from August 2001 to July 2004 and to provide background information for developing and implementing rotavirus vaccine.
METHODSData from hospital-based rotavirus surveillance among children < 5 years old hospitalized with acute diarrhea was used. Patients' clinic information and feces specimens were collected. Specimens were tested and typed for rotavirus.
RESULTSTotally, 3121 specimens were tested and the detection rate of rotavirus was 51%. Among all the children with rotavirus diarrhea, 94% were < 2 years old. G3 (69.9%) was the most prevalent serotype followed by G1 (6.6%) and G2 (2.9%). P[8] was the most common genotype of rotavirus. The most common G-P combination identified in this study was P[8] G3 (64.0%). Seveal other combinations of minor frequency were also identified.
CONCLUSIONRotavirus infection was most commonly seen among children < 5 years old hospitalized with acute diarrhea in the three hospitals. It is important to develop and implement rotavirus vaccine to prevent and control severe rotavirus infection. Because of the diversity of rotavirus strains, it is necessary to perform rotavirus strain surveillance to understand the dynamic nature of viral transmission.
Child, Preschool ; China ; epidemiology ; Diarrhea ; epidemiology ; virology ; Feces ; virology ; Female ; Hospitals ; statistics & numerical data ; Humans ; Infant ; Infant, Newborn ; Male ; Rotavirus Infections ; epidemiology ; virology ; Sentinel Surveillance
10.Three kinds of viruses isolated from diarrhea patients of infants and preschool children in Fuzhou city.
Jian-min GAO ; Tao WANG ; Jun-lin CHEN ; Jun-yang CHEN ; Yu LIN ; Zhongqin CAI
Chinese Journal of Epidemiology 2004;25(5):456-457
Adenovirus Infections, Human
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epidemiology
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virology
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Adenoviruses, Human
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isolation & purification
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Caliciviridae Infections
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epidemiology
;
virology
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Child, Preschool
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China
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epidemiology
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Diarrhea
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epidemiology
;
virology
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Diarrhea, Infantile
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epidemiology
;
virology
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Enzyme-Linked Immunosorbent Assay
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Feces
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virology
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Female
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Gastroenteritis
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epidemiology
;
virology
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Humans
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Incidence
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Infant
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Male
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Norwalk virus
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isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Rotavirus
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isolation & purification
;
Rotavirus Infections
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epidemiology
;
virology