The study used 300 E. coli strains isolated from specimens: stool (150), urine (40), blood (30), billiary fluid (40), pus (40) to find sensitivity and specificity of multiplex PCR in diagnosis of E. coli strains causing diarrhea and distribution of E. coli which have ability causing diarrhea in these specimens. DNA was extracted from E. coli control strains, reference strains belong to Enterobacteiaceaer and E. coli strains from Karolinska Institute (Sweden) and then carrying out multiplex (8 primer pairs) PCR reaction.
Diarrhea ; diagnosis ; Escherichia coli

To identify the viral cause in children with acute diarrhoea in §ucTrong hospital - LamDong province, 158 stool specimens of children with Acute diarrhoea have been collected and identified for rotavirus, adenovirus and snow mountain virus. The result were compared with 151 stool specimens of healthy children in the same time for 3 years (1994-1997). Results of the study showed: the viral positive was found in 52.2% of the diarrheal group. While it was 1.3% in the Healthy Control Group. Among the viral positive, the rotavirus is 50%, Astrovirus is 1.26%, snow mountain virus is 0.67% and adeno virus is 0.63%. For three years, three types of rotavirus has been identified in DucTrong, which are: Rotavirus type I (23/79), Rotavirus type II (13/79), rotavirus type IV (21/79) undifferent (22/79). The rotavirus type III has not been found in this time. The clinical characteristic of the viral diarrheal group has been found in 94.4% of children under 2 years, 81% of cases had vomiting. The dehydration including degree B and C according to the classification of WHO is from 84.74% to 95.34%.
diagnosis ; Diarrhea ; child ; Viruses

Of total 1125 diarrheal fecal samples examined. There were 163 specimens positive by using PRC (14.5%), and 67 Shigella found by culture (5.9%). The positive samples have been characterized by mucus, blood and leukocytes. The results showed that 49% of positive cases by PCR, and 61% of the positive cases by culture had mucus/blood or mucus or blood in the stool. Meanwhile, 18% positive cases by PCR, and 11.9% of positive cases by culture had no mucus, blood and leukocytes in the stool. Together with clinical signs, mucous, bloody and leukocyte characteristics in the stool was useful tools for clinicians in the diagnosis of shigellosis.
diagnosis ; diarrhea ; Feces ; Dysentery

Child ; Diarrhea ; diagnosis ; therapy ; Humans

PURPOSE: The results of a subtotal colectomy in colonic inertia, even those reported in the most recent literature, vary dramatically. The purpose of this study was to assess the effect of a colonic transit study on the results of a subtotal colectomy in colonic inertia. METHODS: Between 1992 and 1997, 30 patients underwent a subtotal colectomy with ileorectal anastomosis due to colonic inertia. Twenty-one of them underwent a colonic transit study only one time preoperatively, and 9 patients underwent such a study two times. The success rate was calculated using traditional definition with two sets of criteria. We modi fied the criteria of success by including new symptoms, such as abdominal, pelvic, or rectal pain, difficult evacuation, and loose stool or diarrhea. The success rate was recalculated using our more stringent cri teria, and compared between group 1 and group 2. RESULTS: By the traditional definition, the functional success rate was 100% for patients undergoing a colonic transit study two times preoperatively and 90% for patients undergoing only one study (p=0.34). By our more stringent criteria, the success rate was 100% for patients undergoing studies two times and 62% for those undergoing only one study (p=0.03). CONCLUSIONS: The success rate of a colectomy for colonic inertia was significantly higher for patients who underwent a repeat transit study than for those who underwent a single study. Patients who have two or more transit studies to confirm the diagnosis of inertia have a significantly higher probability of postoperative improvement of both bowel frequency and associated symptoms, such as pain and difficult evacuation. The mechanism for this discriminatory effect of repeated colonic transit studies requires elucidation by further study.
Colectomy* ; Colon* ; Constipation* ; Diagnosis ; Diarrhea ; Humans

Study 300 children with acute diarrhea treated at Saint Paul Hospital, Hanoi, the results showed that major cause was E.coli (13.7%); most common were EAEC and EIEC, followed by ETEC and EPEC, prevalence of EHEC was low (0.7%). Most of patients were under 1 year of age, and this disease common occurred in summer and autumn. Common symptoms were abdominal pain, watery and loose diarrhea; other symptoms such as fever, vomit and nausea occurred in half of patients.
Diagnosis ; Child ; Epidemiology ; Diarrhea ; Escherichia coli

Child ; Consensus ; Diarrhea ; diagnosis ; therapy ; Humans

The present paper describes two Korean male infants, 1. 16 year old and newly born neonate from two families who were diagnosed and managed for one of very rare inborn errors of metabolism, congenital chloridorrhea (Darrow-Gamble syndrome). The diagnosis was suggested by one of the authors (HRM) from the unusual combination of metabolic alkalosis with severe gastrointestinal disorder presenting with chronic, profuse watery diarrhea in the newborn period in the first patient; and the maternal polyhydramnios, the appearance of dilated fetal bowel loops on prenatal ultrasonography and profuse watery diarrhea beginning at birth without passage of meconium in the second patient. The diagnosis was confirmed in both patients by examination of the stool chloride concentration which revealed extremely high exceeding the sum of sodium and potassium concentrations. Serum electrolytes and arterial blood gas analyses revealed hyponatremia, hypokalemia and hypochloremia with elevated bicarbonate. With replacement of fluid and electrolyte deficit and adequate dietary supplements of potassium and chloride, both patients remained well although the character of the stools waxed and waned. This is the first reported case of congenital chloridorrhea in korean population.
Adolescent ; Chlorides/*metabolism ; Diagnosis, Differential ; Diarrhea/*congenital/diagnosis/ethnology ; Diarrhea, Infantile/*diagnosis/ethnology/metabolism ; Humans ; Infant, Newborn ; Korea ; Male

The present paper describes two Korean male infants, 1. 16 year old and newly born neonate from two families who were diagnosed and managed for one of very rare inborn errors of metabolism, congenital chloridorrhea (Darrow-Gamble syndrome). The diagnosis was suggested by one of the authors (HRM) from the unusual combination of metabolic alkalosis with severe gastrointestinal disorder presenting with chronic, profuse watery diarrhea in the newborn period in the first patient; and the maternal polyhydramnios, the appearance of dilated fetal bowel loops on prenatal ultrasonography and profuse watery diarrhea beginning at birth without passage of meconium in the second patient. The diagnosis was confirmed in both patients by examination of the stool chloride concentration which revealed extremely high exceeding the sum of sodium and potassium concentrations. Serum electrolytes and arterial blood gas analyses revealed hyponatremia, hypokalemia and hypochloremia with elevated bicarbonate. With replacement of fluid and electrolyte deficit and adequate dietary supplements of potassium and chloride, both patients remained well although the character of the stools waxed and waned. This is the first reported case of congenital chloridorrhea in korean population.
Adolescent ; Chlorides/*metabolism ; Diagnosis, Differential ; Diarrhea/*congenital/diagnosis/ethnology ; Diarrhea, Infantile/*diagnosis/ethnology/metabolism ; Humans ; Infant, Newborn ; Korea ; Male

No abstract available.
Colitis, Ulcerative* ; Diagnosis* ; Diarrhea ; Inflammatory Bowel Diseases ; Ulcer*