E. coli is an emerging diarrheagenic pathogen. Serotyping is important to identify bacteria and trace their epidemiology. Objectives: (1) To identify the distribution of serogroup O among 4 kinds of diarrheagenic E. coli strains isolated from children with and without diarrhea in Hanoi, Vietnam. (2)To find out the relationship between serogroup O and different diarrheagenic E. coli. Method: One hundred and sixty-two strains of diarrheagenic E. coli including 86 EAEC, 50 EPEC, 12 EIEC, and 14 ETEC have been serotyped by slide agglutination with antisera specific for O serogroups. Results: More than 60% of the strains were serotyped. Serogroup O25 was the most common with the prevalence of 16.7% followed by O15 (4.3%). The other serogroups (O127a, O128, O126, O6, and O16) were seen in very few numbers. 38.3% of E. coli strains were not serotypeable with available antisera. EAEC were the most prevalent among diarrheagenic E. coli and 25.6% of them belong to the serogroup O25. Conclusion: 60% of the diarrheagenic E. coli strains were serotyped. Serogroup 025 was the most common. The findings were very useful for assessing the distribution of serogroups of diarrheagenic E. coli and will be useful for future vaccine development.
Diarrhea, Escherichia coli, Child

Study on 2483 children aged 0-5 years old with acute diarrhea at Ba Vi district (Ha Tay province), St Paul Hospital, and Thanh Tri district (Ha Noi). Results: prevalence of children aged 0-5 years old with acute diarrhea due to ETEC accounted for 6.4% of all diarrhea children. Majority of diarrhea children caused by ETEC alone (82.3%), most of ETEC diarrhea patients were due to LT ETEC (61.4%). ETEC-related diarrhea characterized loose stool, mild and moderate dehydration, fatigue (83.1%), anorexia (74.65%), and abdominal pain (70.6%). Disease developed quickly, with the mean time of 3.23±1.13 days. The prevalence is higher in summer and autumn.
Diarrhea ; Child ; Escherichia coli

Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli are an emerging agent of pathogens causing diarrhea. Among 587 fecal samples from Vietnamese children under five years of age with diarrhea, the PCR technique identified 68 samples (11.6%) positive with EAEC and 18(7.2%) among the 249 age-matched controls showing the significant difference. In the diarrhea group, the significantly higher prevalence was seen in the children less than two years of age as compared to the older ones. There is a decreasing tend of EAEC infection during the age. When comparing the children at the age of less than 12 months, the prevalence of EAEC was significantly higher in the diarrhea group than in the control. More studies need to be carried out to verify the role of EAEC in causing diarrhea in children.
Diarrhea ; Escherichia coli ; Child

Study 300 children with acute diarrhea treated at Saint Paul Hospital, Hanoi, the results showed that major cause was E.coli (13.7%); most common were EAEC and EIEC, followed by ETEC and EPEC, prevalence of EHEC was low (0.7%). Most of patients were under 1 year of age, and this disease common occurred in summer and autumn. Common symptoms were abdominal pain, watery and loose diarrhea; other symptoms such as fever, vomit and nausea occurred in half of patients.
Diagnosis ; Child ; Epidemiology ; Diarrhea ; Escherichia coli

600 pediatric patients including 300 children with diarrhoea were treated at Ha Noi St Paul Hospital. There were a high rate of E.coli causing diarrhoea in children (21%) among than EACE had got the highest (8%) and EHEC the lowest (0,5%). E.coli was highly sensitive to antibiotics of the groups quinolone cefatexin, amikacin and was highly resistent with chloramphenicol, co.trimoxazole. E.coli could be multi resistent to 6 antibiotics, but the most common was 2 antibioresistancies.
Drug Resistance, Microbial ; Escherichia coli ; Child ; Diarrhea

Child ; China ; Diarrhea ; diagnosis ; microbiology ; Escherichia coli Infections ; diagnosis ; Humans ; Shiga-Toxigenic Escherichia coli ; isolation & purification

OBJECTIVETo investigate the etiologic value of diarrheagenic E. coil harboring genomic O island 28(OI-28) containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635), which were related to RTX (Repeat in toxin) toxin family isolated from children with diarrheal disease in Taiyuan.

METHODSIn the study, 257 fecal samples from children with diarrheal disease collected in Shanxi Children's Hospital. Diarrheagenic E. coli and enteropathogenic bacteria were isolated and identified by conventional bacterial culture and typing specific diarrheagenic E. coli (EPEC, EIEC, ETEC and EHEC) diagnostic serum, while diarrheagenic E. coli harboring genomic 01-28 containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635) were detected by PCR and DNA southern blot hybridization.

RESULTS206 strains (80.16%) of enteropathogenic bacteria were detected from 257 children with diarrhea disease, containing 149 strains (57.98%) of diarrheagenic E. coli and 57 strains(22.18%) of other entero-pathogenic bacteria. Among 3 strains (2.01%) of EPEC, 2 strains (1.34%) of ETEC, 2 strains (1.34%) EHEC were detected by typing specific serum, while all of the 142 strains (95.30%) isolated were suspected to be diarrheagenic E. coli. 21 strains (14.09%) of diarrheagenic E. coil harboring genomic O1-28 containing five putative virulence genes (Z0608, Z0609, Z0615, Z0634 and Z0635) were detected by polymerase chain reaction and DNA southen blot hybridization, 8 strains (5.37%) of diarrheagenic E. coli containing only one genomic OI-28 virulence gene, 2 strains (1.34%) of diarrheagenic E. coli containing two genomic OI-28 virulence gene. 21 children with diarrhea diseases caused OI-28-harboring E. coli containing five important putative virulence genes were among 0 to 3 years old (80.95%). These children correlating with OI-28-harboring E. coli did not present special clinical symptoms or signs.

CONCLUSIONThe diarrheagenic E. coil harboring genomic OI-28 was one of the important etiology for children with diarrheal disease in summer season.

Child ; China ; Diarrhea ; microbiology ; Escherichia coli ; genetics ; pathogenicity ; Escherichia coli Infections ; complications ; Genes, Bacterial ; Humans ; Virulence

Verotoxin-producing Escherichia coli O157 is a primary cause of severe and bloody diarrhea. Campylobacter spp. are one of the commonly reported bacterial cause of gastrointestinal infections throughout the world. Only a few cases involving both E. coli O157 and Campylobacter species have been reported. The authors simultaneously isolated verotoxin-producing E. coli O157 and Campylobacter species from the stool of a 3 year-old male with bloody diarrhea, fever and abdominal pain.
Abdominal Pain ; Campylobacter* ; Child, Preschool ; Diarrhea ; Escherichia coli O157* ; Escherichia coli* ; Escherichia* ; Fever ; Gastroenteritis* ; Humans ; Male ; Shiga Toxins ; Shiga-Toxigenic Escherichia coli

Escherichia coli O157 is an important serotype of enterohemorrhagic E. coli that causes hemorrhagic colitis worldwide. Outbreaks of E. coli O157 have been assocoated with contaminated food like meat, raw milk, and water, but recently vegetables and fruits have accounted for a growing number of recognized outbreaks. We isolated verotoxin producing E. coli O157 from the stool of a 3 year-old female with bloody diarrhea and abdominal pain. The child had been eating salad with vegetables and fruits frequently.
Abdominal Pain ; Child ; Colitis ; Diarrhea ; Disease Outbreaks ; Eating ; Enterohemorrhagic Escherichia coli ; Escherichia ; Escherichia coli ; Escherichia coli O157 ; Female ; Fruit ; Humans ; Meat ; Milk ; Shiga Toxins ; Vegetables

OBJECTIVETo investigate the proportion of hemorrhagic colitis (HC) caused by Escherichia coli O157:H7 in bacterial diarrhea in Xuzhou city, Jiangsu province.

METHODSAll stool samples from patients with diarrhea were screened for O157 antigen, using Immuno-gold kits. Positive samples were cultured to detect the existence of pathogens. All of the HC patients confirmed by bacterial isolation and identification were investigated for clinical symptoms and laboratory tests.

RESULTSOf the diarrhea patients identified in Feng county in May, and in Tongshan county of Xuzhou city in June 2000, Jiangsu province 0.98% and 5.89% were caused by Escherichia coli O157:H7 respectively, confirmed by bacteriological isolation and identification of stool samples. At the early phase of hemorrhagic colitis, 18.5% patients had at least one abnormal clinical laboratory test results including protein in urea and increased BUN or creatinine that indicating the possibility of kidney damage. In 27 strains of E. coli O157:H7 isolated from those patients, 13 and 14 were identified as Shiga toxin producing and Shiga-toxin negative E. coli O157:H7 (Stx-positive or Stx-negative) respectively. By analysis of the two groups of patients divided by according to the nature of Shiga toxin, four of 13 patients of Stx-positive group showed positive urea protein. However only 1 of the 13 patients of Stx-negative group was urea protein positive. The decreased Platelets counts were observed in 6 of 13 patients with Stx-positive group, but only in 1 of 14 patients with stx-negative group. These differences were statistically significant.

CONCLUSIONHC patients caused by E. coli O157:H7 were commonly seen (up to 5.89%) in Xuzhou city, Jiangsu province. Early laboratory tests should be conducted for HC patients as early as possible in order to find early indictor of kidney failure which was critical for prevention of hemolytic uremic syndrome.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Diarrhea ; etiology ; Escherichia coli Infections ; etiology ; Escherichia coli O157 ; isolation & purification ; Female ; Humans ; Infant ; Male ; Middle Aged