Objective To investigate the inhibitory effect of proliferation and invasiveness on PANC1 cell by knockdown of NF-κB P65 expression through RNA interference. Methods Chemically synthesized small interference RNA (siRNA) directed against human NF-κB p65 was transfected into pancreatic carcinoma cell (PANC1) by using cationic liposome LipofectamineTM 2000 as the transfection agent. A non-silencing siRNA was used as the negative control and distilled water was used as the blank control. The tests repeated for six times. The expression of NF-KB p65 and ICAM-1 gone was detected by RT-PCR. The effect of cell proliferation and invasive ability was evaluated by MTF, clony assay, Matrigel invasive tests. Results The relative expression levels of NF-κB p65 mRNA in siRNA, the negative control and the blank control group were 0.227 ±0.045, O. 381 ± 0. 038 and 0. 404 ± 0. 031, respectively; the relative expression levels of ICAM-1mRNA were 0.597 ± 0. 083, 0.983 ± 0.068 and 1. 027 ± 0.098, respectively; the inhibitory rate of the cells at 72h were 18.3%, 2.3% and 0, respectively; the colony forming efficiency were ( 14.1 ± 3.1 ) %, ( 24.5±2.1)% and (27.2 ±2.6)%, respectively; the number of invasive cells were 80.25 ±6.35, 123.83 ±8.80 and 127.68 ± 9.23, respectively; the difference between the siRNA group and two control groups was significant (P ＜ 0. 01 ). Conclusions Down-regulatiag the expression of NF-κB p65 gene by RNAi may inhibit proliferation and invasiveness in pancreatic carcinoma cell strain PANC1.

Conventional digital video acquisition card can only collect but not process video frequency.This paper introduces the design of a digital video acquisition card based on C6000 DSP chip of TI company,which can process digital video automatically and communicate with mainframe through PCI interface.

As a part of modern medical practice,medical treatment facility(MTF) has playing an important role in disease diagnosis and medical treatment;therefore,nowadays more attention is paid to the medical equipment management.The methods of equipment management which combine actuality and practical experience are introduced in order to make the management of medical equipment more scientific and effective.

To analyze and study the influences of temperature and humidity to the medical equipments, and indicate the problem and strategy of the medical equipment in the operation. To give some references in installation and maintenance of facilities.

Objective To get a better medical image segmentation result by studing a new image segmentation method. Methods Medical images were segmented using image segmentation method based on texture character and generalized radial basis function neural networks. The texture character parameters were obtained according to gray level co-occurrence matrix. The parameters were input to the generalized radial basis function neural networks to train the network. Results Comparatively, perfect binary images were obtained by using this new image segmentation method. Conclusion The emulational results show that the method is an effective medical image segmentation method.

ObjectiveTo study the expression and potential significance of NET-1 in endometrial carcinoma.Methods106 specimens after operation were selected.All specimens were stained with H.E.and immunohistochemistry.ResultsThe expression of NET-1 immunoreactivity were different in normal endometrium(43.8％ ),endometrial hyperplasia ( 79.2％ ) and endometrial carcinoma ( 100％ ) ( x2 =11.88,P ＜ 0.01 ).The expression of NET-1 in endometrial carcinoma was significantly higher than that in normal endometrium and simple endometrial hyperplasia ( x2 =36.14,10.86,P ＜ 0.01 ).The expression of NET-1 in endometrial hyperplasia was significantly higher than that in normal endometrium ( x2 =5.29,P＜ 0.05 ).There was a correlation between the expression of NET-1 and degree of endometrial lesions ( r =0.355,P ＜0.01 ).The expression of NET-1 was relative with differentiation (x2 =11.04,P ＜0.01 ; r =0.558,P ＜0.01),stages(x2 =11.04,P ＜0.01; r =0.558,P ＜0.01),depth of myometrial invasion (x2 =15.90,P ＜0.01 ; r =0.479,P ＜0.01),with or without metastasis in lymph node ( Z =-3.74,P ＜0.01 ),menopause condition ( Z.=-2.60,P ＜0.01).ConclusionsNET-1 may play an important role in carcinogenesis and progression of endometrial carcinoma.It might be a marker of biological behavior and reference for endometrial carcinoma gene therapy.

Objectives:To study the effects of gene therapy with tissue type plasminogen activator(t PA)cDNA on the formation of thrombo embolism in vascular anastomotic sites. Methods:①The cDNA encoding t PA was amplified by RT PCR using the isolated total RNA as the template from the Bowes melanoma cells.②Recombinant plasmid pAdCMV t PA was cotransfected into 293 cells with pJMa 17 ,and the infectious but replication deficient AdCMV t PA was generated.③The rats were randomly divided into the control and treatment groups.11 0 nylone medical suture was applied to perform rat carotid artery end to end anastomoses.In the treatment group,AdCMV t PA solution was injected into the vascular anastomotic site while AdCMV (no containing t PA DNA) solution was injected into the control group. By means of RT PCR and chromogenic plasmin substrates,the following results were obtained. Results:①The t PA cDNA was successfully cloned and its eukaryotic expressing vector was constructed.②When the isolated RNA was performed with RT PCR,1.69 kb band appeared in the treatment group while the band could not be found in the control group.The t PA activity could be detected postoperatively on the 1st,2 nd,3 rd,4 th,5 th,6 th,7 th,10 th and 13 th day of the treatment,but could not be detected in the control group. Conclusions:The t PA gene can produce t PA having biological activity at anastomotic sites, possibly prevent the formation of thrombus embolism effectively and develop the anastomotic patency.

BACKGROUND: Composite with different ratios has various elastic noduli, and this can cause stress protection in implanted body so as to influence on biological integration between composite and host bone and plerosis of bone defection.OBJECTIVE: To measure elastic modulus of tissue engineering bone made of composite between true bone ceramic (TBC) granule and bone cement (BC) and provide experimental data for repairing bone defection at various sites.DESIGN: Controlled study.SETTING: Military Institute of Bone Tumor, Tangdu Hospital of the Fourth Military Medical University of Chinese PLA.MATERIALS: The experiment was carried out in the Military Institute of Bone Tumor, Tangdu Hospital of the Fourth Military Medical niversity of Chinese PLA from August 2002 to March 2003. Ten Kunming mice,weighing 10-15 g, of both genders, were selected in this study. Fresh longtubular cortical bone of 1-year calf, 6 samples of spongy bone at inferior extremity and 6 samples at iddle femur of fresh corpse were also selected in this study.METHODS: Bovine bone morphogenetic protein (bBMP) was xtracted to measure osteogenic-induced activity and make TBC granule. bBMP was mixed with TBC as the ratio of 1:25, and then with BC as the ratios of 0:10, 4:6, 5:5,6:4 and 7.5:2.5. In addition, elastic modulus of tissue engineering bone made of TBC-BC composite was measured and compared with elastic modulus of femur and spongy bone of normal adult males.MAIN OUTCOME MEASURES: Measurement and comparison of elastic modulus.RESULTS: Elastic modulus of composite containing 60% TBC (Ratio between TBC and BC was 6:4) was no significantly different from that of spongy bone at inferior extremity of femur of normal adult males (P ＞ 0.05),but other comparisons of every two subjects were significant difference [femur of the adults: (6.216 7±0.222 9) Mpa; spongy bone: (1.351 7±0.306 9) Mpa;TBC/BC (0:10): (5.710 0±0.166 3) Mpa; TBC/BC (4:6): (3.510 1±0.205 0) Mpa;TBC/BC (5:5): (2.004 1±0.150 0) Mpa; TBC/BC (6:4): (1.501 8±0.005 7) Mpa;TBC/BC (7.5:2.5): (0.900 4±0.025 1) Mpa, P ＜ 0.01].CONCLUSION: Elastic modulus of composite containing 60% tissue engineering bone made of TBC-BC composite is similar to that of spongy bone at inferior extremity of femur of adult males. The composite can be used to repair bone defection near by articular facet so as to prevent from articular degeneration.