The variation in hardness of enamel is a frequently used method to evaluate the influence of whiting materials on the enamel. The purpose of this study is to improve the veracity on the evaluation tests caused by the tooth itself with point selection method. Three kinds of testing point selection methods on enamel were carried out, i. e. random selection, grid measurement and symmetrical measurement, respectively. The selected points were used to measure the micro hardness by Vickers. The influence of the variation of tooth structure itself on the hardness measurements results can be reduced by using testing point selection methods of symmetry, and thus, the accuracy of the test method used in the evaluation of the influence of dental materials on tooth hardness will be guaranteed.
Animals ; Cattle ; Dental Enamel ; drug effects ; Dental Stress Analysis ; Dentin ; drug effects ; Hardness ; drug effects ; In Vitro Techniques ; Tooth Bleaching ; methods ; Tooth Crown ; drug effects

The many-banded krait, Bungarus multicinctus, has been recorded as the animal resource of JinQianBaiHuaShe in the Chinese Pharmacopoeia. Characterization of its venoms classified chief phyla of modern animal neurotoxins. However, the evolutionary origin and diversification of its neurotoxins as well as biosynthesis of its active compounds remain largely unknown due to the lack of its high-quality genome. Here, we present the 1.58 Gbp genome of B. multicinctus assembled into 18 chromosomes with contig/scaffold N50 of 7.53 Mbp/149.8 Mbp. Major bungarotoxin-coding genes were clustered within genome by family and found to be associated with ancient local duplications. The truncation of glycosylphosphatidylinositol anchor in the 3'-terminal of a LY6E paralog released modern three-finger toxins (3FTxs) from membrane tethering before the Colubroidea divergence. Subsequent expansion and mutations diversified and recruited these 3FTxs. After the cobra/krait divergence, the modern unit-B of β-bungarotoxin emerged with an extra cysteine residue. A subsequent point substitution in unit-A enabled the β-bungarotoxin covalent linkage. The B. multicinctus gene expression, chromatin topological organization, and histone modification characteristics were featured by transcriptome, proteome, chromatin conformation capture sequencing, and ChIP-seq. The results highlighted that venom production was under a sophisticated regulation. Our findings provide new insights into snake neurotoxin research, meanwhile will facilitate antivenom development, toxin-driven drug discovery and the quality control of JinQianBaiHuaShe.