OBJECTIVETo establish an induced pluripotent stem (iPS) cell line from the skin fibroblasts of a patient with acute lung injury (ALI-iPS).

METHODSWe obtained iPS cells from a female ALI patient by forced expression of a cocktail of transcription factors (Sox2, Klf4, Oct4 and Nanog) into the dermal fibroblasts to result in reprogramming to a pluripotent state. The ALI-iPS cells generated were analyzed for colony morphology, alkaline phosphatase (AP) activity, surface antigens, and differentiation ability.

RESULTSThe ALI-iPS cells exhibited morphological and growth characteristics of ES cells, showed positivity for alkaline phosphatase by histochemical staining, and expressed ES cell marker genes. Subcutaneous injection of iPS cells into immunodeficient (SCID) mice resulted in tumors containing a variety of tissues from all the 3 germ layers.

CONCLUSIONThe ALI patient-specific iPS cell line was successfully established to serve as a valuable model to study the cellular pathology of acute lung injury and develop high-throughput drug screening assays.

Acute Lung Injury ; Animals ; Cell Line ; Cellular Reprogramming ; Culture Media ; chemistry ; Female ; Fibroblasts ; cytology ; Gene Expression ; Humans ; Induced Pluripotent Stem Cells ; cytology ; Mice ; Mice, SCID ; Skin ; cytology ; Transcription Factors ; chemistry