Objective:Clinical study in the treatment of common bile duct stones combined with ERCP and EST.Methods:the October 2014-2017 year in February for hepatobiliary surgery inour hospital after ERCP combined with LC in the treatment of patients with cholecystolithiasis and choledocholithiasis in 158 cases.According to ERCP postoperative LC interval difference divided into ERCP definite operation LC group(104 cases) and ERCP Elective operation LC surgery group(54 cases).The definite operation for ERCP LC group is LC line 24h-72h,another is the patients were givenLC for 3 months after ERCP.The clinical efficacy,LC operation time,hemorrhage,the gallstone is discharged into the bile duct,recurrent cholecystitis,bile leakage,length of stay and medical fee were compared between the two groups (ERCP definite operation LC group of single hospitalization time;ERCP Elective operation LC surgery group of two hospital hours together),average ERCP times,medical expenses.Results:all of the two groups were cured after operation.length of sta is long and medical expensive which was selected a time to do (P＜0.05).Conclusion:ERCP definite operation of LC group in the treatment of common bile duct stones and gallbladder stones surgery is safe,less medical expenses,hospitalization time is short,patients recover quickly,the burden is small.

This study was aimed to investigate the protective effects of dimethylsulfoxide (DMSO) combined with trehalose on the cryopreserved platelets. The platelets were preserved at -80 degrees C. The experiments were divided into 5 groups: blank control group composed of apheresis platelet suspension; trehalose group composed of apheresis platelet suspension and 0.25 mol/L trehalose; DMSO group composed of apheresis platelet suspension and 5% DMSO; 5% combined group composed of apheresis platelet suspension, 5% DMSO and 0.25 mol/L trehalose; 2.5% combined group composed of apheresis platelet suspension, 2.5% DMSO and 0.25 mol/L trehalose. All the groups were thawed at 37 degrees C in a waterbath. The recovery rate of platelets and mean platelet volume (MPV) were assayed by using hemocytometer; the ultrastructural changes were examined by electron microscopy; the expressions of CD41, CD42b, CD61 and CD62p on platelets were detected by flow cytometry. The results indicated that single use of trehalose had no strong effect in increasing the recovery rate of platelets, but the morphology of platelets was close to normal. The DMSO showed significant effect in increasing the recovery rate of platelets and maintaining the intact property of platelets, however, the shape of platelets tended to sealing, and partial platelets still displayed heteromorphic changes. The combination of DMSO and trehalose revealed the protective effect on the external morphology and internal structure of platelets to be close to the normal homeostasis, and ensured an ideal recovery rate of the cryopreserved platelets and higher expression levels of CD41, CD42b, CD61 and CD62p in the same time. It is concluded that the combined use of DMSO and trehalose possesses the synergistic protective effect on the cryopreserved platelets, therefore, the combined use of both as the protective agent is hopeful to further raise the effectiveness of clinical infusion of the cryopreserved platelets.
Blood Platelets ; drug effects ; Blood Preservation ; methods ; Cryopreservation ; methods ; Dimethyl Sulfoxide ; pharmacology ; Humans ; Platelet Count ; Trehalose ; pharmacology

Objective To study the short-term impact of cleft palate repair surgery on hearing and middle ear function in infants .Methods Thirty-three infants with cleft palate who received cleft palate repair surgery from January 2013 to November 2014 in Peking Union Medical College Hospital were included in this study , in-cluding 22 male infants and 11 female infants .They were examined using distortion product otoacoustic emission (DPOAE), auditory brainstem response (ABR), 226 Hz probe-tone tympanometry before surgery and 1 -24 months after surgery .Results The mean age of the 33 infants (66 ears) at the time of surgery was 17 months (8.5-25 months).Sixty-four ears were examined with preoperative DPOAE , with 7 ears (10.9%) passed;postoperative DPOAE was performed in 56 ears, with 19 ears (33.9%) passed.The passing rate was significant-ly improved after operation ( P<0.01 ) .In the 54 ears for which both preoperative and postoperative DPOAE were conducted , 16 ears ( 29.6%) showed improved hearing .Sixty-three ears were examined with preoperative ABR, showing a mean hearing threshold of (43.73 ±13.65 ) dB nHL;62 ears were examined with postoperative ABR, showing a mean hearing threshold of (35.65 ±13.75 ) dB nHL, significantly improved compared with the preoperative threshold ( P<0.01 ) .In the 53 ears which received both preoperative and postoperative ABR , 29 ears (54.7%) showed reduced hearing thresholds .There was no significant difference in 226 Hz probe-tone tympanometry between preoperative and postoperative examinations ( P>0.05 ) .Conclusion In infants with cleft palate , short-term hearing improvement may be observed after repair surgery , while middle ear function may show no obvious improvement .

OBJECTIVETo study the audiological and otological status of cleft palate infants with the application of distortion product otoacoustic emission (DPOAE), auditory brainstem response (ABR), tympanometry with 1000 Hz and 226 Hz probe tones.

METHODSTotally 45 cleft palate infants aged 8-24 months were included in the study. Most of them were examined for DPOAE, ABR and two frequency tympanometry.

RESULTSMost infants failed the three tests,among whom 6.7% ears passed DPOAE and 33.3% of ears had normal ABR hearing threshold. In addition, 8.9% of ears turned out normal in the 1000 Hz probe-tone tympanometry, and 13.3% were type A in the 226 Hz probe-tone tympanometry. Finally, 1000 Hz tympanometry had more agreement with DPOAE and latency of ABR wave I than 226 Hz tympanometry.

CONCLUSIONMost cleft palate infants have audiological and otological problems, which should be evaluated in a more comprehensive manner.

Acoustic Impedance Tests ; Cleft Palate ; physiopathology ; Evoked Potentials, Auditory, Brain Stem ; Humans ; Infant ; Infant, Newborn ; Neonatal Screening ; Otoacoustic Emissions, Spontaneous