Objective To study the relation between the quantity of DNA extracted by Chelex-100 method and multiplex STRs analysis.Methods DNA extracted from a variety of common forensic casework specimens were quantified by using Real-time PCR,and then amplified with AmpFLSTR IdentifilerTM PCR Amplification kit.ResultsAccording to the results of quantification,the quantities of DNA extracted from 113 samples by Chelex-100 method were adjusted to 0.5~3ng for establishing 8?l amplification system,and in this condition,most of 113 forensic casework specimens could be successfully genotyped.Conclusion When the quantity of DNA extracted by Chelex-100 method ranged from 0.5ng to 3ng,most results of multiplex STRs analysis were satisfying.Moreover,the amplification effect of 1?l DNA template was better than 3?l DNA template when the concentrations of extracted DNA were more than 0.5ng/?l.

Acupuncture Therapy ; Adolescent ; Adult ; Ataxia ; congenital ; drug therapy ; therapy ; Child ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Moxibustion ; Spinal Cord Diseases ; congenital ; drug therapy ; therapy ; Young Adult

Objective To study the genetic polymorphism of 15 STR loci of 3 populations in Guangxi province. Methords DNA samples of unrelated individuals from 314 Gelao population,332 Mulao population,238 Yao population were analyzed using AmpFlSTR IdentifilerTM PCR Amplification Kit and 3100 Genetic Analyzer. Results The matching probability of the 15 STR loci was 1.839 ?10-16 ~ 5.073 ? 10 -17 and combined paternity of exclusion was 0.9999983 ~ 0.9999991 in the 3 populations. Conclusion The results showed that the 15 STR loci in Identifiler?PCR Amplification systems were useful for forensic case works in Gelao population, Mulao population and Yao population .

10.3969/j.issn.1007-3969.2013.05.003

Objective To compare the specificities of the cell membrane stationary phases(CMSP) with cell membrane chromatography(CMC).Methods Cell chromatographic columns were constructed for both rat aorta tissue cells and cultured rat aorta smooth muscle cells.Then the chromatographic affinities of ten ligands of ?-adrenergic receptor(?-AR) with both said chromatographic columns were investigated.Capacity factors(k'),as a chromatographic parameter,were calculated.Results The correlation analysis showed a positive correlation between the rat aorta tissue CMSP and the cultured rat aorta smooth muscle cell CMSP,with correlation factor of r=0.923,P

Objective To discuss causes and corresponding prevention for groin pain occurred early after total hip arthroplasty(THA).Methods A retrospective study was done on 189 cases(193 hips)treated with THA including unilateral procedures in 185 hips and bilateral procedures in eight hips to analyze common causes for early groin pain.Results Groin pain was found in 9.3% hips(18/ 193)during hospital stay,including 1.6%(3 cases)with deep infection,1.6%(3 cases)with incision infection,1%(2 cases)with posterior dislocation,4.1%(8 cases)with leg lengthening and 1%(2 cases)with hematoma.Conclusions Despite of the numerous diagnostic alternatives available to the orthopedic surgeon,detailed history,careful physical examination,necessary laboratory and imaging stud- ies can contribute to a correct determination of causes for groin pain.Meanwhile,appropriate indication, accurate preoperative radiographic measurement,intraoperative standardized surgical procedures and per- fect rehabilitation are necessary to avoid complications.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Bloodletting ; Female ; Headache ; therapy ; Humans ; Male ; Young Adult

Objective To investigate the distribution of neurons expressing Fos protein in central nervous system (CNS) following esophageal mucosal acid exposure,and to map the contribution of spe- cific brain areas in sensitizing responsivity and emphasize the coding change of CNS to the esophageal acid stimulation.Methods Thirty-six healthy Sprague-Dwley rats were randomly divided into five groups. Group A (n=6) was normal group of home cage control animals to which no stimulation was given. Group B (n=7) was saline group which received esophageal perfusion with normal saline solution (0.9% NaCl).Group C (n=8) was treated with esophageal mucosal acid exposure containing 0.1 mol/L HCl. Group D (n=7) was sensitized by ovalbumin.Group E (n=8) received basal ovalbumin-sensitization plus esophageal mucosal acid exposure.The rat model of esophageal visceral hypersensitivity was estab- lished by the basic ovalbumin-sensitization combined with intra-esophageal mucosal acid exposure.The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer under various conditions.Results The rats in model group with basic ovalbumin-sensitization plus esophageal acid perfusion initiated a high density expression of c-fos- immunoreactive(Fos-IR) neurons in multineuronal networks.A significantly higher number of Fos positive neurons was found in the model group than those in the corresponding regions of other groups (P＜0.05) in the following brain areas:frontal and parietal cortex,insular cortex,cingulated cortex,central amyg- daloid nucleus,the K(?)lliker-Fuse nucleus,the nucleus ambiguus,parabrachial nucleus,hypothalamic paraventricular nucleus,paraventricular thalamic nucleus,paratrigeminal nucleus,the nucleus of solitary tract,area postrema,reticular nucleus of medulla,whereas no significant difference was found in the dorsal motor nucleus of the vagus,supraoptic nucleus,periaqueductal gray matter or orbital part of infe- rior frontal gyrus.The values of Fos-IR neurons were also increased in the central amygdala,parabrachi- al nucleus,paraventricular nucleus,the paratrigeminal nucleus and NTS in the model group than that in the corresponding regions of other groups (P＜0.05).Conclusion The basic ovalbumin-sensitization fa- cilitated dramatically the c-fos expression evoked by esophageal acid perfusion,suggesting that visceral hypersensitivity induced by ovalbumin may alter cortical reactivity processing of esophageal acid stimula- tion in brain areas.

OBJECTIVETo compare the therapeutic effect of electroacupuncture and western medication on blood pressure variability in young patients with hypertension.

METHODSSixty cases of hypertension in young patients were randomized into an electroacupuncture group and a western medication group, 30 cases in each one. In electroacupuncture group, electroacupuncture was applied on Quchi (LI 11) and Taichong (LR 3). In western medication group, Captopril was taken orally. Twenty-four hour dynamic blood pressure, blood pressure variability and day-night rhythm were observed after 14 days treatment in two groups.

RESULTSElectroacupuncture on Quchi (LI 11) and Taichong (LR 3) reduced systolic pressure and diastolic pressure at different time phases in young patients with hypertension (all P < 0.01), which did not present significant statistical difference as compared with western medication group (all P > 0.05). Electroacupuncture on Quchi (Li, 11) and Taichong (LR 3) reduced apparently the standard deviations of systolic pressure and diastolic pressure at different time phases in young patients with hypertension, indicating significant statistical differences as compared with the deviations before the treatment (P < 0.01, P < 0.05). The statistical significance presented in 24 h systolic standard deviation (24 h SSD), 24 h diastolic standard deviation (24 h DSD), daytime SSD (dSSD), nighttime SSD (nSSD) and nighttime DSD (nDSD) between two groups (P < 0.01, P < 0.05). In comparison of the numbers of Dippers and non-Dippers in two groups, the significant statistical difference presented between two groups after treatment (P < 0.05).

CONCLUSIONElectroacupuncture on Quchi (LI 11) and Taichong (LR 3) has long-term antihypertensive effect and improves effectively day-night rhythm variation in young patients with hypertension. Quchi (LI 11) and Taichong (LR 3) are the effective pair points for hypertension treated with acupuncture.

Acupuncture Points ; Adult ; Blood Pressure ; Electroacupuncture ; Female ; Humans ; Hypertension ; physiopathology ; therapy ; Male ; Middle Aged

OBJECTIVETo probe into using evidence-based medicine to establish TCM stroke unit, so as to serve for clinical treatment of apoplexy.

METHODSBased on basic theories of TCM and in referred to the stroke unit model with therapeutic effect confirmed by evidence-based medicine, TCM stroke unit with characteristics of acupuncture and moxibustion was established.

CONCLUSIONEstablishment of TCM stroke unit with TCM characteristics is objective in future, but it still needs high quality of clinical evidences.

Acupuncture Therapy ; Evidence-Based Medicine ; Humans ; Medicine, Chinese Traditional ; Moxibustion ; Stroke