Objective To analyze the constituent and clinical features of the senile females who underwent surgery for gynecological tumors and traumatic diseases,so as to enhance the surgical safety and efficacy.Methods From Jan.2003 to Dec.2006,a total of 178 female patients,who were undergone surgery for gynecological tumors or traumatic diseases,were involved in present study,of whom the clinical data,the patients' constituent and pre-operative complications were retrospectively analyzed.The cases selected were allocated into two groups according to their age:the observation group included 116 patients with age ≥60,among them 86 cases were undergone operation for gynecological tumors and 30 cases for traumatic diseases;the control group included 62 patients with age of 40-50 and comparable parity and body weight,among them 54 cases were undergone operation for gynecological tumors and 8 cases for traumatic diseases.Results The number of the patients operated for gynecologic tumors remained the majority annually,whereas a yearly increase of traumatic diseases was noticed with 3.1 and 1.8 folds increasing in 2006 compared to that in 2003 and 2004,respectively(P

Objective:To study the changes of plasma orphanin (OFQ) level in post-partum depression (PPD). Methods:The level of plasma OFQ of 24 patients with post-partum depression (PPD group) and 25 healthy lying-in women (control group) were measured by radioimmunoassay.Results:The level of plasma OFQ in PPD group was 27.39?6.04 ng/L , and that of control group was 10.37?3.65 ng/L,the difference was statistically significant(P

BACKGROUND: Orphanin (OFQ) is associated with ischemia/hypoxia,which may play an important role in the production and development of fetal distress and neonatal asphyxia.OBJECTIVE: To observe the change of OFQ content in hypothalamus and peripheral blood of intrauterine ischemia/hypoxia fetal rats and analyze the role of OFQ in the perinatal ischemia/hypoxia.DESIGN: Randomized controlled animal trial.SETTING: Department of Obstetrics and Gynecology, Changhai Hospital of the Second Military Medical University of Chinese PLA.MATERIALS: The experiment was performed at the Department of Obstetrics and Gynecology, Changhai Hospital of the Secon d Military Medical University of Chinese PLA from May 2002 to September 2003. A total of 12 Wistar female rats, with the mean body mass of 260 g were selected and fed routinely [provided by the experiment animal center of this university, number of certificate scxk(Hu)2002/0006].METHODS: The 12 female rats were randomized into three groups: control group, ischemia/hypoxia for 10 minutes group, ischemia/hypoxia for 20 minutes group with 4 rats in each group. Female rats in each group were pregnant. On day 21 of pregnancy, female rats in each group were cut the belly open, and the uterine vessels were incarcerated for 10 minutes in the ischemia/hypoxia for 10 minutes group with 21 fetal rats and 20 minutes in the ischemia/hypoxia for 20 minutes group with 17 fetal rats, respectively. Fetal rats were directly obtained from control group with 19 ones. None of fetal rats died. All the fetal rats received Apgar score and decapitation. The blood of trunk was collected and the whole brain was obtained. OFQ content in hypothalamus and peripheral blood was measured with radioimmunoassay.MAIN OUTCOME MEASURES: OFQ content in hypothalamus and peripheral blood of fetal rats in each group.RESULTS: Totally 57 rats were involved in the result analysis. ①The levels ofOFQ in hypothalamus and peripheral blood were (71±14) pg/g and (31±7) ng/L in ischemia/hypoxia for 10 minutes group, (114±21) pg/g and (58±11) ng/L in ischemia/hypoxia for 20 minutes group, (48±9) pg/g and (19±4) ng/L in the control group. Compared with the control group, the levels of OFQ in hypothalamus and peripheralblood increased in the ischemia/hypoxia for 10 minutes group and ischemia/hypoxia for 20 minutes group (P＜ 0.05, P ＜ 0.01), and it in ischemia/hypoxia for 10 minutes group was significantly higher than that in ischemia/hypoxia for 20 minutes group (P ＜ 0.05). ②The score of Apgar was lower in the two groups than in the control group (P ＜ 0.01 ), of which it was lower in the ischemia/hypoxia for 20minutes group than in the ischemia/hypoxia for 10 minutes group (P ＜ 0.05).CONCLUSION: The perinatal ischemia and hypoxia can induce the increase of OFQ content in hypothalamus and peripheral blood.

BACKGROUND: Endogenous opioid peptide is an important medium and regulator that participate in many physical and pathologic processes of the body. Its relationship with fetal encephalopathy has attracted much attraction.OBJECTIVE: To evaluate the role of endogenous opioid peptides (EOP) in fetal distress.DESIGN: A case-control observatory study based on healthy pregnant women.SETTING: Wards of the Department of Obstetrics and Gynecology of Changzheng Hospital Affiliated to Second Military Medical University of Chinese PLA.PARTICIPANTS: Eighty-three healthy women who were hospitalised in Changzheng Hospital and Changhai Hospital affiliated to the Second Military Medical University of Chinese PLA and met the inclusion criteria. Among them, 40 were normal healthy pregnant women(the control group) and 43 were healthy pregnant women with fetal distress(the fetal distress group).METHODS: Radioimmunoassay was used to measure the levels of blood EOP(β-endorphin, dynorphin A1- 13 and leu-enkephalin) of the venous blood of the pregnant women in fetal distress group and the control group and the EOP level in the umbilical blood of the newborns. Also, blood gas analysis of the blood from the umbilical artery was conducted.MAIN OUTCOME MEASURES: The levels of EOP in the venous blood of two groups of pregnant women and the umbilical blood of newborns and the correlation of EOP level with fetal distress.RESULTS: The levels of the umbilical artery blood EOP(β-endorphin,dynorphin A1-13 and leu-enkephalin) in the fetal distress group[(453± 68 ) ng/L, (242 ± 33)ng/L, and(498 ± 68)ng/L respectively] were significantly higher than those in the control group[ (251 ± 39) ng/L, (103± 22 )ng / L and(322 ± 40 )ng / L respectively ( t = 2. 713,2. 762, P＜ 0.01; t = 2. 132, P ＜ 0.05 ) ]. The umbilical artery blood gas analysis;pH was (7.0 ± 0. 1 ) , PO2 was ( 1.7 ± 0.6) kPa, PCO2 was (8.9 ± 0. 7) kPa.The levels of β-endorphin were negatively correlated with pH and PO2 of the umbilical artery blood(r= -0.418 and -0.437, P ＜ 0.01), but they were positively correlated with PCO2( r = 0. 442, P ＜ 0. 05) . The level of dynorphin A1-13 was negatively correlated with pH and PO2( r = -0. 337,-0.383, P ＜ 0.05), but it was positively correlated with PCO2(r= 0. 346, P ＜ 0. 05). There was no significant difference among the three kinds of blood EOP of the two groups( P ＞ 0.05).CONCLUSION: EOP participates in the pathological progress of the fetal distress and was closely correlated with the occurrence and development of the fetal distress. This finding has a reference value for early rehabilitation and intervention after the fetal was born that can be tested quantitatively.

Objective To evaluate the clinical value of transesophageal echocardiography (TEE) on minimally invasive surgical closure of heart septal defects. Methods Thirty-four patients with atrial septal defect (ASD) and 38 patients with ventricular septal defect(VSD) were selected by transthoracic echocardiography (TTE) prepared for minimally invasive surgical closure. TEE was performed to choose appropriate occluder and guide occluder release during the procedure. The immediate closure effect also evaluated by using TEE. A week follow-up was done by using TTE. Results Successful occlusion was in 32 patients with ASD and 29 patients with VSD. On 1 week follow-up,neither displacement for the occluders nor residual shunt except minimal residual shunt in 3 patients. The ventrieular remodeling was improved, the valvular regurgitation and pulmonary arterial pressure decreased. Conclusions TEE is important in minimally invasive surgical closure of atrial, ventricular septal defects, especially in choosing the candidate for the procedure and occluder, guiding occluder released and evaluating the procedure.

Objective To provide theoretic base by studying the nature and characteristic of infrared ther- mograph in lower limbs area of the healthy young man.Methods Collecting infrared thermograph in lower limbs ar- ea by TIP medical infrared thermograpb instrument (TIP,made in USA) ,the average temperature of each unit area of both lower limbs was measured.Results (1) The average temperature of each corresponding unit area in both side of lower limbs was adjacent (P＜0.05 ).(2) The temperature of both lower limbs (especial front and back side) distributinged in some rule.Conclusion The study provided parameter and characteristic of infrared therrnograph for clinical diagnosis and guideline of rehabilitation therapy by infrared thermograph instrument.

Objective To provide an observational baseline by studying the nature and characteristics of in- frared thermograms of the spine area of the healthy young men.Methods Infrared thermograms of the spine area were collected using a TIP medical infrared thermograph.The average temperature of each unit area in the middle and on the two sides of the spine was measured.Results (1)Except certain unit areas in the cervical thorax and lum- bar sacral segments,the average temperature of each unit area in the middle of the spine was significantly higher than on the two sides.In the middle of the spine,the average temperature of the cervical thorax and lumbar sacral seg- ments was insignificantly higher than adjacent areas.The shape of the infrared thermograms of the cervical thorax and lumbar sacral segments resembled a triangle and a rhomboid,respectively.Conclusion The study provided para- meters and characteristics of infrared thermograms for clinical diagnosis and guidelines for rehabilitation therapy using an infrared thermograph.

OBJECTIVE: To optimize the formulation of iodoform paste for root canal filling of deciduous teeth. METHODS: Using multi-indicators comprehensive scoring method, which takes the delicate degree, lubricity, difficulty of root canal filling, and difficulty of removal as performance indicators, the matrix type was screened, and the formulation of paste was optimized by orthogonal experimental design. The content of iodoform in the optimized paste was determined by UV spectrophotometry. RESULTS: Dimethicone was chosen as the paste matrix by comprehensive scoring of indicators. The results of orthogonal test showed that the ratio of iodoform and zinc oxide was the main factor affecting the performance of the paste, and the optimal formulation of paste was as follows: the ratio of iodoformto zinc oxide 3:7, clove oil 0.5%, and the ratio of powder to liquid 70:30.The linear range of the calibration curve for iodoform in the optimal paste was 48.75-146.22 μg·mL-1, A = 6.781 3C-0.051 6(r=0.999 6); and the average recovery rate was 99.83% (n=9). CONCLUSION: The optimal iodoform paste has a simple preparation, fine and smooth for root canal filling of deciduous teeth. The assay method is accurate and reliable for the quality control of iodoform paste.

In order to identify Peucedani Radix, Peucedani Decursivi Radix and their adulterants, the internal transcribed spacer 2 (ITS2) regions of Peucedani Radix, Peucedani Decursivi Radix and their adulterants were amplified and bidirectionally sequenced based on the Principles for Molecular Identification of Traditional Chinese Materia Medica Using DNA Barcoding, which has been promulgated by Chinese Pharmacopoeia Commission. Sequences were analyzed and assembled by Codon Code Aligner V3. 7.1. The relevant data were analyzed by MEGA 5. 0. Species identification analyses were performed by using the nearest distance methods and neighbor-joining (NJ) methods. The result showed that the ITS2 sequence lengths of Peucedani Radix were 229-230 bp and the average intra-specific genetic distances were 0.005. The ITS2 sequence lengths of Peucedani Decursivi Radix were 227 bp and the sequences contained no variation site. The average inter-specific K2P genetic distance of Peucedani Radix, Peucedani Decursivi Radix and their adulterants species were 0.044 and 0.065 respectively. The minimum inter-specific divergence is larger than the maximum intra-specific divergence of Peucedani Decursivi Radix. The nearest distance methods and NJ trees results indicated that Peucedani Radix, Peucedani Decursivi Radix and their adulterants species could be identification clearly. The ITS2 regions can stably and accurately distinguish Peucedani Radix, Peucedani Decursivi Radix and their adulterants.
Apiaceae ; classification ; genetics ; DNA Barcoding, Taxonomic ; methods ; DNA, Ribosomal Spacer ; Drug Contamination

Objective To evaluate the usefulness of real-time three-dimensional transesophageal echocardiography (RT-3D TEE) in the perventricular device occlusion of the ventricular septal defect (VSD).Methods Sixty patients underwent perventricular device occlusion of VSD were divided into two groups.Group A:30 patients,3D images of VSD were obtained intraoperatively by RT-3D TEE.3D images were analyzed and the maximal and minimum area and maximal diameter of VSD were measured in the cardiac cycle.The size of selected VSD occluder was the diameter calculated from the maximal area based on the formula πD2/4 and plus 1～2 mm.Simultaneously,two dimensional transesophageal echocardiography (2D TEE) images of VSD were obtained and measured on standard views.Group B:other 30 patients,intraoperative 2D TEE was performed to measure the diameter of VSD on standard views.The selection VSD occluder was based on the maximal diameter plus 1～2 mm.Results The VSDs shape showed oval,class round and irregular by RT-3D TEE imaging.There was a significant difference between the maximal area and smallest area of VSD appeared in the cardiac cycle obtained by RT-3D TEE (P<0.01).There was a significant difference between the maximal diameter by measurement of RT-3D TEE and 2D TEE (P<0.01).Of all 30 patients with evaluation of VSD size based on the RT-3D TEE,1.1 times attempts of device occlusion were performed on one patient (33/30).Of the other 30 cases with evaluation of VSD size by 2D TEE,2 cases were failed with device occlusion because of instant residual shunt.Of the remaining 28 cases,1.43 attempts of device occlusion were performed on one patient (40/28).Conclusions RT-3D TEE can accurately reveal the size of VSD,and aid in the selection of VSD occluder in the device occluding procedure of VSD.