2.Hyperglycemia in poor controlled diabetes from crude tamarind herbal pill: a case study.
Asian Pacific Journal of Tropical Biomedicine 2011;1(1):79-80
Hyperglycemia is an important finding in the diabetic patient with poor glycemic control. There are several possible causes of hyperglycemic. Here, the author presents an interesting case study on a female diabetic patient presenting with hyperglycemic due to intake of crude tamarind herbal pill. General practitioner should realize that the use of alternative medicine can be a cause of unexplained hyperglycemic episode in diabetic patient.
Diabetes Mellitus
;
Humans
;
Hyperglycemia
;
chemically induced
;
Male
;
Middle Aged
;
Plant Extracts
;
adverse effects
;
Tamarindus
;
chemistry
4.The Effect of Pertussis Vaccine and Cyclosporin on Streptozotocin Induced Diabetic Rats.
Yonsei Medical Journal 1987;28(2):143-151
The injection of streptozotocin(stz) at a high dose (60 mg/kg) into young male rats produces direct beta cell destruction and leads to insulin dependent diabetes (IDD). In contrast the injection of multiple smal doses of stz (40 mg/Kg/d for 5 days) produce IDD, which resembles type l diabetes in man. The provocative effects of the pertussis vaccine (PV) and cyclosporin(CA) against the development of IDD induced by stz were studied. When PV in a dose of 3.75 X 10(10) microorganism was administered to single or multiple stz treated rats, hyperglycemia still developed and persisted during the experiment. No difference was noted in blood glucose levels, but plasma insulin levels were higher in PV treated rats. When CA (10 mg/kg) was administered daily to single or multiple stz treated rats, hyperglycemia seemed to be lower, but this was not statistically significant, however, plasma insulin levels were higher in CA treated rats. The results of this experiment suggest that PV and CA provide some protection to the beta cells of the pancreas.
Animal
;
Blood Glucose/metabolism
;
Cyclosporins/pharmacology*
;
Diabetes Mellitus, Experimental/prevention & control*
;
Diabetes Mellitus, Insulin-Dependent/chemically induced
;
Male
;
Pertussis Vaccine/pharmacology*
;
Rats
;
Rats, Inbred Strains
5.Optimization of streptozotocin dosing for establishing tumor-bearing diabetic mouse models.
Yao TANG ; Xianghui LEI ; Wenjing JIAN ; Jinhai YAN ; Ziqing WU ; Tong ZHAO
Journal of Southern Medical University 2014;34(6):827-831
OBJECTIVETo determine the optimal dosing of streptozotocin (STZ) for establishing lymphoma-bearing diabetic mouse models.
METHODSA total of 200 healthy male Balb/c mice were randomized into 4 groups (n=50) for intraperitoneal injection of a single dose of vehicle solution (control) or 75, 150, or 200 mg/kg STZ. The changes of body weight and blood glucose were observed regularly, and the success rate of modeling, mortality rate, and survival of the mice were recorded after the injections. The mice with successfully induced diabetes received subcutaneous or tail vein injection of A20 lymphoma cells, and the rate of tumorigenesis, mortality rate, and survival time were observed at 1 month and 3 months after tumor cell injection.
RESULTSCompared with the control group, the mice receiving STZ injection at 150 and 200 mg/kg showed significantly decreased body weight and increased blood glucose (P<0.05), while STZ at 75 mg/kg did not produced such obvious changes. STZ injection at 200 mg/kg resulted in a significantly higher mortality rate and shorter survival time than STZ at 150 mg/kg (P<0.05). In the control group and 150 and 200 mg/kg STZ groups, the rate of tumorigenesis or mortality rate showed no significant differences after subcutaneous injection of A20 lymphoma cells (P>0.05), but differed significantly at 3 months after tail vein injection of the tumor cells (P<0.05).
CONCLUSIONIntraperitoneal injection of STZ at 150 mg/kg is associated with a low mortality rate, a high successful modeling rate of diabetes and a long survival time in mice, and is therefore optimal for establishing diabetic mouse models bearing transplanted tumors.
Animals ; Blood Glucose ; Body Weight ; Diabetes Mellitus, Experimental ; Diabetes Mellitus, Type 2 ; chemically induced ; Injections ; Male ; Mice ; Mice, Inbred BALB C ; Streptozocin ; administration & dosage
6.Study on pancreas of type 1 diabetic mice induced by MLD-STZ using proteomics.
Miao GENG ; Hong-yan CHEN ; Jian-hua WANG ; Ya-zhuo HU ; Gang ZHANG
Chinese Journal of Applied Physiology 2011;27(3):357-361
OBJECTIVETo get a better understanding of the mechanisms underlying type 1 diabetes mellitus, the differentially expressed pancreatic proteins from multiple low-dose streptozotocin (MLD-SIZ) mouse and normal mouse were analyzed and compared.
METHODS20 male rats were separated into 2 groups (n=10): model mice treated with MLD-STZ and normal mice,differences of pancreatic proteome among in the two groups of mice, were analyzed by two dimensional polyacryamide gel electrophoresis (2DE). Protein quantification was analyzed and the differentially expressed spots were identified using mass spectrometry and MASCOT database searching.
RESULTSCompared with control group, 23 proteins had changed significantly in the model group, 8 proteins expression were up-regulated, 15 proteins expressions down-regulated significantly. Using MALDI-TOF-MS, 15 proteins with significant change were identified by peptide fingerprinting map and the results were searched in MASCOT database. The function analyzed showed that proteins with change were associated with metabolic, anti-oxidant, structural, catalytic enzymes and chaperone, et al.
CONCLUSIONType 1 diabetes is probably exerted via multi-target and multi-path mechanism. The proteins with significant change are newly target for type 1 diabetes early diagnosis and treatment.
Animals ; Diabetes Mellitus, Experimental ; physiopathology ; Diabetes Mellitus, Type 1 ; chemically induced ; metabolism ; physiopathology ; Male ; Mice ; Pancreas ; metabolism ; Proteins ; metabolism ; Proteomics ; methods ; Streptozocin
7.Time-dependent changes of calbindin D-28K and parvalbumin immunoreactivity in the hippocampus of rats with streptozotocin-induced type 1 diabetes.
Journal of Veterinary Science 2013;14(4):373-380
The hippocampus is affected by various stimuli that include hyperglycemia, depression, and ischemia. Calcium-binding proteins (CaBPs) have protective roles in the response to such stimuli. However, little is known about the expression of CaBPs under diabetic conditions. This study was conducted to examine alterations in the physiological parameters with type 1 diabetes induced with streptozotocin (STZ) as well as time-dependent changes in the expression of two CaBPs changes of were being evaluated. Rats treated with STZ (70 mg/kg) had high blood glucose levels (>21.4 mmol/L) along with increased food intake and water consumption volumes compared to the sham controls. In contrast, body weight of the animals treated with STZ was significantly reduced compared to the sham group. CB-specific immunoreactivity was generally increased in the hippocampal CA1 region and granule cell layer of the dentate gyrus (DG) 2 weeks after STZ treatment, but decreased thereafter in these regions. In contrast, the number of PV-immunoreactive neurons and fibers was unchanged in the hippocampus and DG 2 weeks after STZ treatment. However, this number subsequently decreased over time. These results suggest that CB and PV expression is lowest 3 weeks after STZ administration, and these deficits lead to disturbances in calcium homeostasis.
Animals
;
Calbindin 1/*genetics/metabolism
;
Diabetes Mellitus, Experimental/*chemically induced
;
Diabetes Mellitus, Type 1/*chemically induced
;
*Gene Expression Regulation
;
Hippocampus/*metabolism
;
Male
;
Parvalbumins/*genetics/metabolism
;
Rats
;
Rats, Wistar
;
Streptozocin/administration & dosage
10.The Link between Exposure to Phthalates and Type 2 Diabetes Mellitus: A Study Based on NHANES Data and Bioinformatic Analysis.
Xue Kui LIU ; Shan Wen SI ; Yan YE ; Jia Yi LI ; He He LYU ; Ya Mei MA ; Cai Yan ZOU ; Hao Jie SUN ; Lei XUE ; Wei XU ; Hou Fa GENG ; Jun LIANG
Biomedical and Environmental Sciences 2023;36(9):892-896