1.Effect of rosiglitazone on tumor necrosis factor-alpha-induced nuclear factor-kappaB and coupling factor 6 expressions in human umbilical vein endothelial cells.
Ze-bing YE ; Zhi-liang LI ; Shu-dong SONG ; Di-guang PAN ; Qiang FU ; Ying-feng LIU
Journal of Southern Medical University 2008;28(9):1642-1645
OBJECTIVETo investigate the effect of rosiglitazone on the expression of nuclear factor-kappaB (NF-kappaB) and coupling factor 6 (CF6) induced by tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical vein endothelial cells (HUVEC).
METHODSCultured HUVEC of passage 3-5 were stimulated with TNF-alpha and then cultured in the presence of rosiglitazone. The expression of CF6 and NF-kappaB subunit p65 were evaluated by immunocytochemistical method.
RESULTSPretreatment of HUVECs with rosiglitazone inhibited TNF-alpha-induced expression of CF6 in a dose-dependent manner. The activation of CF6 stimulated by TNF-alpha was suppressed by ROS in a dose-dependent manner.
CONCLUSIONTNF-alpha-induced enhancement of the gene expression and release of CF6 is mediated by activation of NF-kappaB signaling pathway. ROS can inhibit the activation of IKK, block NF-kappaB signaling pathway and inhibit the expression of CF6, which may be the mechanism underlying the action of TZDs on hypertension.
Cells, Cultured ; Endothelial Cells ; cytology ; drug effects ; metabolism ; Humans ; Hypoglycemic Agents ; pharmacology ; Immunohistochemistry ; Mitochondrial Proton-Translocating ATPases ; biosynthesis ; NF-kappa B ; biosynthesis ; Oxidative Phosphorylation Coupling Factors ; biosynthesis ; Thiazolidinediones ; pharmacology ; Tumor Necrosis Factor-alpha ; pharmacology ; Umbilical Veins ; cytology
2.Studies on the chemical constituents from the aerial parts of Cimicifuga dahurica.
Yong LIU ; Di-hua CHEN ; Jian-yong SI ; Rui-le PAN ; Guang-zhong TU ; Dong-ge AN
Acta Pharmaceutica Sinica 2003;38(10):763-766
AIMTo seek for new bioactive constituents from the aerial parts of Cimicifuga dahurica.
METHODSVarious column chromatographic techniques were employed for the isolation and purification of the ingredients. The structures were elucidated on the basis of 1H, 13CNMR, 1H-1H COSY, HMQC, NOESY and HMBC spectra and chemical reactions.
RESULTSTwo cyclolanostanol xylosides, cimidahuside C and D were isolated from the EtOAc section of EtOH extracts.
CONCLUSIONCimidahuside C(1) and D(2) are new triterpenoid xylosides.
Cimicifuga ; chemistry ; Glycosides ; chemistry ; isolation & purification ; Molecular Structure ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification
3.Direct reprogramming of porcine fibroblasts to neural progenitor cells.
Xiu-Ling XU ; Ji-Ping YANG ; Li-Na FU ; Ruo-Tong REN ; Fei YI ; Keiichiro SUZUKI ; Kai LIU ; Zhi-Chao DING ; Jing QU ; Wei-Qi ZHANG ; Ying LI ; Ting-Ting YUAN ; Guo-Hong YUAN ; Li-Na SUI ; Di GUAN ; Shun-Lei DUAN ; Hui-Ze PAN ; Ping WANG ; Xi-Ping ZHU ; Nuria MONTSERRAT ; Ming LI ; Rui-Jun BAI ; Lin LIU ; Juan Carlos IZPISUA BELMONTE ; Guang-Hui LIU
Protein & Cell 2014;5(1):4-7
Animals
;
Cellular Reprogramming
;
Dentate Gyrus
;
cytology
;
Fibroblasts
;
cytology
;
Mice
;
Neural Stem Cells
;
cytology
;
transplantation
;
Swine