1.Study on the Low Positive Results of Detecting HBsAg in Serum Specimens with Roche Cobas e602 and Modular e170 Electrochemical Luminescence Analyzer
Ya LI ; Di ZHANG ; Yun ZHANG ; Hai HUANG ; Mingquan SU
Journal of Modern Laboratory Medicine 2017;32(3):123-125
Objective To investigate the correlation between the results of HBsAg positive results among different detection systems,and provide reference for the analysis of clinical test results and the publication of the report.Methods The HBsAg positive serum specimens with the quantitation result lower than 80 COI were detected by Roche Cobas e602 electrochemical luminescence analyzer.All the specimens were also detected by Roche Modular e170 electrochemical luminescence analyzer.The differences of detecting results were compared and performed the linear correlation analysis.Results The experimental results of two detection systems are R2 =0.933.The positive coincidences of Group A,B,C,D and E were 60.60%,92.72%,96.66%,96.66% and 100%,respectively.The positive coincidence of the males was 87.66%,while the positive coincidence of the females was 70.96 %.The positive coincidence of the males was significantly higher than the females (P<0.05).Conclusion The HBsAg detecting results of Roche Cobas e602 and Roche Modular e170 electrochemical luminescence analyzer had high correlation.The results higher than 10 COI had 100% positive coincidence rate,however the results between 1 and 10 COI were not.The result between 1 and 10 COI may lead to controversial results,suggestions for further checks.
2.Correlation Analysis of HCV-RNA,HCV-Ab and HCV-cAg
Ya LI ; Yun ZHANG ; Hai HUANG ; Di ZHANG ; Mingquan SU ; Xuchang GUO
Journal of Modern Laboratory Medicine 2016;31(5):120-122
Objective To investigate the correlation of HCV-RNA with detection indexes HCV-Ab and HCV-cAg in its clini-cal application effect among patients with hepatitis C.Methods HCV-cAg and HCV-Ab in 140 cases of HCV-RNA were detected by enzyme linked immunosorbent assay in cases of PCR,which were detected by real-time fluorescence quantitative PCR.Results 127 cases in 140 cases of HCV-RNA positive serum were HCV-cAg positive,in line with the rate of 90.71%,and the cases of 110 HCV-Ab positive,in line with the rate of 78.57%.The positive detection rate of HCV-cAg with different HCV-RNA concentration was increased with the increase of HCV virus content,and the serum of different HCV-RNA concentration had no significant changes in HCV-Ab detection results.Conclusion The detection results of HCV-cAg had a high coincidence rate with HCV-RNA.Therefore detection of HCV-cAg can be as a complementary detec-tion of HCV-Ab,as the window period of HCV infection and infection in immunocompromised persons screening provides a simple,inexpensive method.At the same time it provides rapid screening for HCV infection provide diagnostic basis for those basic medical units who do not have the conditions for detection of HCV-RNA.
3.Design of medical positive pressure protective suit for long-voyage aeromedical evacuation
Yu-Juan SU ; Li-Qun WANG ; Ya-Di ZHANG ; Xiang-Yi YANG ; Zhen-Yao SONG
Chinese Medical Equipment Journal 2024;45(11):113-116
Objective To develop a medical positive pressure protective suit for long-voyage aeromedical evacuation to realize protective isolation and drinking water and energy supply for medical personnel during long-voyage aeromedical evacuation of respiratory infectious disease patients.Methods The medical positive pressure protective suit had a one-piece structure,with its main part made of hydroentangled non-woven fabric,head and forebreast parts made of amorphous polyethylene terephthalate anti-fog material and wearing-and taking-off parts sealed with zipper and autohesion,which was equipped with a portable positive pressure air supply device,an airborne centralized positive pressure air supply device and a monitoring and warning device.The portable positive pressure air supply device was fixed in the back of the suit at the waist,the airborne device was made by modifying the commercially available positive pressure air supply fan,and the monitoring and warning device monitored the air supply volume of the fan,the battery power and the pressure inside the suit.Results The suit behaved well in protection,clean fresh air supply without time limitation and facilitating hydration and energy replenishment of medical personnel by forming three activity spaces.Conclusion The suit developed can continuously provide a clean and comfortable microenvironment,meeting the requirements of medical personnel for protection and hydration and energy replenishment during long-voyage aeromedical evacuation.[Chinese Medical Equipment Journal,2024,45(11):113-116]
4.A multicenter comparison study on the quantitative detection of bcr-abl (P210) transcript levels in China.
Ya-zhen QIN ; Hui CHENG ; Jian-nong CEN ; Su-xia GENG ; Qing-hua LI ; Xiao-qing LI ; Zhen-xing LIN ; Dao-xin MA ; Chun QIAO ; Yun-gui WANG ; Jin-lan LI ; Ling-di LI ; Xiao-jun HUANG
Chinese Journal of Hematology 2013;34(2):104-108
OBJECTIVETo investigate the comparability of bcr-abl (P210) transcript levels detected in different hospitals.
METHODSTen hospitals in China took part in the four times of sample exchange and comparisons from April, 2010 to August, 2011. The exchange samples were prepared by Peking University People's Hospital. Firstly, the BCR-ABL (P210)(+) cells from a newly diagnosed chronic myeloid leukemia patient were 10-fold serially diluted by BCR-ABL (P210)(-) cells and they covered 4 magnitudes. Then, TRIzol reagents were thoroughly mixed with cells in each tube. Every 12 samples (three samples per magnitude) were sent to the other 9 hospitals. The cell number of each sample was 8×10(6). The detection of bcr-abl transcript levels by real-time quantitative PCR were performed in every hospital according to their own protocols. Conversion factors (CF) were calculated using regression equation.
RESULTSDifferences in bcr-abl transcript levels did exist among results of 10 hospitals in each comparison. In general, the results of the most of hospitals were in line with the dilutions of cells. CF of every hospital fluctuated. Three hospitals had relatively stable CF, and their ranges were 2.8 - 5.2, 1.2 - 2.8 and 2.2 - 6.8, respectively; two hospitals had unstable CF with ranges 0.76 - 7.0 and 2.1 - 18.7; three hospitals couldn't be calculated CF one or two times because of the significant deviation of the results from the actually bcr-abl transcript levels, and their ranges of CF which could be calculated were 1.9 - 19.2, 3.6 - 7.6 and 0.18 - 14.7; One hospital only had two CF (3.3 and 5.0) because of the replacement of an important reagent during the period of comparisons.
CONCLUSIONSComparability of bcr-abl (P210) transcript levels between different hospitals could be achieved through CF which acquired by sample exchange and comparison. The stable and reliable detection system is the premise to acquire correct CF.
Bone Marrow Cells ; China ; Fusion Proteins, bcr-abl ; genetics ; isolation & purification ; Hospitals ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; diagnosis ; genetics ; Reverse Transcriptase Polymerase Chain Reaction
5.Epidemiological characteristics and spatial clustering of Toxoplasma gondii in domestic cats in China
Ya-Jing SU ; Xue LIN ; Chen ZHANG ; Di XUE
Chinese Journal of Zoonoses 2024;40(10):913-921
We investigated the prevalence and spatial distribution characteristics of T.gondii infection in cats in China,to provide a scientific basis for the prevention and con-trol of T.gondii.We searched PubMed,CNKI,Wanfang Da-tabase,and Baidu Academic Database to retrieve studies on the seroprevalence of T.gondii in domestic cats published by December 31,2023,in China,to investigate the spatial loca-tion and expression of T.gondii infection in China by using Geographical Information Systems(GIS).SPSS 26.0 software was used for statistical analysis.A total of 86 eligible studies published between 1984 and 2023 were included in this study,covering 28 provincial administrative regions and involving 20 722 cats.The ArcGIS classification results indicated that the overall positivity rate of T.gondii in domestic cats was 14.78%(3 080/20 836,95%CI:14.07%-15.03%).No epidemiological study of T.gondii infection in cats has been reported in Tibet,Tianjin,Hainan,Hong Kong,Ma-cao,or Taiwan.The prevalence of T.gondii infection in cats was highest in Shanxi Province(60%,54/90,95%CI:49.82%-70.18%),followed by Hebei Province(57.33%,43/75,95%CI:46.07%-68.59%),Henan Province(45.53%,173/380,95%CI:46.07%-68.59%),and Yunnan Province(43.64%,24/55,95%CI:30.49%-56.79%).The lowest seroprevalence of T.gondii antibody was found in Jiangxi Province(2.47%,2/81,95%CI:-0.90%-5.90%).Furthermore,the seropreva-lence of T.gondii in cats in northern China was 14.12%(2 044/14 491 95%CI:12.34%-15.90%),whereas that in southern China was 16.30%(1 036/6 355 95%CI:15.40%-17.20%).Significant differences were observed between them(x2=16.60,P<0.05).The temporal distribution results indicated a decrease in the overall seroprevalence of T.gondii in cats in China from 1984 to 2023.Our findings suggested that T.gondii in cats is widespread in China,with a stable epidemic cycle,and the rates of Toxoplasma positivity vary greatly among regions.The increasing prevalence of pet cats in China,and the close rela-tionship between these cats and humans,may pose substantial exposure risks to a large susceptible population.Therefore,the association between cat ownership and T.gondii infection in humans,and how to decrease human environmental contamination through cat exposure to T.gondii and T.gondii oocysts,requires further investigation.
6.Endemic plants for medicine use in China.
Gang-Qiang SU ; Hai-Tao LI ; Hui SUN ; Xiao-Bo ZHANG ; Li-Xia ZHANG ; Ya-Jing LI ; Lu-Qi HUANG ; Xiao-Jun MA
China Journal of Chinese Materia Medica 2017;42(22):4329-4335
Based on plant species databases, species lists and literature records, general situation of the Chinese medicinal endemic plant (vascular plant) has been systematically summarized, and its quantity and distribution characteristics of Chinese medicinal endemic plants are presented in this paper. The results showed that 3 150 endemic species are Chinese medicinal plants belonging to 785 genera in 153 families, which includes 38 species of 22 genera in 12 families of pteridophyta, 42 species of 14 genera in7 families of gymnosperms, and 3 070 species of 749 genara in 134 families of angiosperms. The top four families involving medicinal endemic species are Asteraceae (218 species), Ranunculaceae (182 species), Labiatae (151 species), and Liliaceae (133 species). The top four provincial administration distributed medicinal endemic species are Sichuan (1 568 species), Yunnan (1 533 species), Guizhou (955 species) and Hubei (930 species).On the regional scale, the most abundant one is the southwest region (2 465 species), followed by the central region (1 226 species) and the northwest region (949 species). Localization characteristics for domestication and artificial cultivation of medicinal endemic species are more prominent due to their narrower and limited distribution areas, indicating it is possible for these species acting as local potential resource for reasonable economic development.
7.Study on the catalytic mechanism of triterpene C-29 carboxylases from Tripterygium wilfordii based on directed evolution
Pan-ting LIU ; Yi-feng ZHANG ; Yuan LIU ; Jie GAO ; Lin MA ; Xiao-yi WU ; Ya-ting HU ; Ping SU ; Shi-jun YUAN ; Xia-nan ZHANG ; Wei GAO
Acta Pharmaceutica Sinica 2024;59(6):1883-1893
Celastrol and wilforlide A are the main active triterpenoids of the traditional Chinese medicine Lei Gong Teng, which have anti-tumour, anti-inflammatory and immunosuppressive activities, and are the material basis for the clinical efficacy of Lei Gong Teng-related Chinese medicinal preparations. By analysing the biosynthetic pathway of active ingredients, optimizing genetic elements and utilizing "cell factory" to produce triterpenoids heterologously will be an effective way to obtain from
8.Analysis of Preoperative Factors Influencing Hypoglossal-facial 'Side'-to-side Neurorrhaphy for Facial Paralysis after Excision of Acoustic Neuroma.
Di Ya SU ; Hong WAN ; De Zhi LI ; Hui QIAO ; Michael SCHUMACHER ; Song LIU
Biomedical and Environmental Sciences 2020;33(1):30-36
Objective:
Hypoglossal nerve-facial nerve 'side'-to-side neurorrhaphy is a new method for the treatment of potential incomplete facial paralysis after acoustic neuroma. However, there are differences in postoperative outcomes among patients. This study analysed preoperative factors that may influence the treatment outcomes of neurorrhaphy.
Methods:
We performed a retrospective study of 53 patients who were treated by neurorrhaphy for facial paralysis after acoustic neuroma resection. After a one-year follow-up period, the patients were divided into two groups according to facial functional outcome: better recovery or ordinary recovery. We analysed the following factors: gender, age, tumour size, and characteristics, tumour adhesion to the facial nerve, the duration of facial paralysis (DFP) and F wave appearance prior to neurorrhaphy (F wave).
Results:
Univariate analysis showed significant differences between the two groups in DFP ( = 0.0002), tumour adhesion to the facial nerve ( = 0.0079) and F waves ( = 0.0048). Logistic regression analysis of these factors also showed statistical significance with values of 0.042 for the DFP, 0.043 for F waves, and 0.031 for tumour adhesion to the facial nerve.
Conclusions
Tumour adhesion to the facial nerve, F waves appearance and DFP prior to neurorrhaphy are the predominant factors that influence treatment outcomes.
9.Clinical, molecular and cytogenetic characteristics of newly diagnosed adult acute myeloid patients with TP53 gene mutation.
Wen Min CHEN ; Hong LIU ; Ling Di LI ; Ling Yu LONG ; Yue Yun LAI ; Hong Xia SHI ; Xiao Su ZHAO ; Hao JIANG ; Qian JIANG ; Yan Rong LIU ; Ya Zhen QIN
Chinese Journal of Hematology 2019;40(6):528-531
10.Liquid Chromatography-Tandem Mass Spectrometry-Based Characterization of Steroid Hormone Profiles in Healthy 6 to 14-Year-Old Male Children.
Bing-Yan CAO ; Chun-Xiu GONG ; Di WU ; Xue-Jun LIANG ; Wen-Jing LI ; Min LIU ; Chang SU ; Miao QIN ; Xi MENG ; Jia-Jia CHEN ; Li-Ya WEI
Chinese Medical Journal 2018;131(7):862-866