2.Sperm DNA fragmentation index and the success rate of IVF/ICSI.
Di XI ; Yun CHEN ; Yu-tian DAI
National Journal of Andrology 2016;22(1):77-81
Sperm DNA fragmentation index (DFI) refers to the percentage of DNA strand breaks in the total sperm. Many studies suggest that elevated DFI can lead to male infertility and early spontaneous abortion. High-DFI patients are more likely to fail in assisted reproduction and preliminary treatment or prevention methods have been developed for this population. This review focuses on the impact of DFI on clinical pregnancy outcomes and progress in the studies of its treatment.
Abortion, Spontaneous
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Chromatin
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DNA Fragmentation
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Female
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Humans
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Infertility, Male
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Male
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Pregnancy
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Pregnancy Outcome
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Sperm Injections, Intracytoplasmic
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Spermatozoa
3.Determination of Camphor in Compound Menthol Nose Drop by RP-HPLC
Jing TIAN ; Yalin XU ; Di YANG
China Pharmacy 2001;0(12):-
OBJECTIVE:To determine camphor in compound menthol nose drop by RP-HPLC.METHODS:Waters No-va-pak C 18 was taken as the chromatographic column,the mobile phase was composed of chloroform-methanol-water(14∶52∶30)with a flow speed at1.0ml/min and detection wavelength at289nm,the column temperature was35℃.RESULTS:The linear range for camphor was0.8~5.6?g/ml(r=0.9998)and the average recovery rate was99.4%(RSD=0.7%).CONCLUSION:The method is specific,convenient and accurate,which can be used for the quality control of the preparation.
4.Molecular dynamics simulation and analysis of BAFF which is incorporated with p-nitro-L-phenylalanine
Di CAI ; Hong TIAN ; Wenbing YAO
Journal of China Pharmaceutical University 2017;48(2):227-232
In order to study the structure-function relationship in the protein which is incorporated with p-nitro-L-phenylalanine,the method of MD(Molecular Dynamics) simulation was established and successfully used in the analysis of protein which contains p-nitro-L-phenylalanine.The force field of CHARMM can only stimulate protein with natural amino acid in NAMD.Compared with phenylalanine,p-nitro-L-phenylalanine just has one more group of nitro.If the parameter of group of nitro was defined,the protein containing p-nitro-L-phenylalanine can be simulated.CGenFF-paramchem was used to calculate the energy and topological structure of p-nitro-L-phenylalanine' s new bonds (r),angles (θ),dihendrals (φ) and improper angle (ψ).And then the new defined parameter and topology information was input into the related parameter files and topology files in CHARMM.On the basis of correct parameter,NAMD can successfully simulate the modified BAFF(B lymphocyte stimulator) which contains p-nitro-L-phenylalanine.The changes in structure indicated that there might be new B cell epitopes.The temperature distribution of each frame in the process of dynamics stimulation was in accord with normal distribution,which proved the defined force field parameters was feasible.The RMSD of whole protein solution systemis 2.5.Calculate each resides' RMSF in BAFF,the RMSF of p-nitro-L-phenylalanine's residue is 3.7,which is obviously higher than that of the other residues in β-pleated sheet,and close to the loop rings,indicate that there might be variation in the area of p-nitro-L-phenylalanine residue and might produce new comformational epitopes.The results of MD stimulation will guide the immunogenicity experiments of p-nitro-L-phenylalanine modified proteins.
5.Investigation of health knowledge and behaviors on clonorchiasis sinensis of urban residents in Yanqing District, Beijing City
Shuguang ZHANG ; Lili TIAN ; Di WAN
Chinese Journal of Schistosomiasis Control 2017;29(4):490-493
Objective To understand the status of health knowledge and behaviors on clonorchiasis sinensis of urban resi-dents in Yanqing District of Beijing City,China,so as to provide the targeted suggestions for further health education. Meth-ods The communities/streets were selected as the investigation sites by using the stratified randomly sampling method. A total of 283 residents were randomly selected and investigated with the structural questionnaire designed by Beijing Center for Dis-ease Control and Prevention. The fecal samples were collected and the eggs of Clonorchis sinensis were detected with Kato-Katz technique. Results All of the questionnaires were valid. The awareness rate about clonorchiasis was 25.09%. The rates of know-ing clonorchiasis infection route,harm and prevention measures were 10.60%,9.89%and 10.60%respectively. Totally 7.07%of the residents had the habits of eating raw or undercooked fresh water fish,and 2.47%of the residents had the behavior of eat-ing sashimi. C. sinensis eggs were not found among the research objects. Conclusions The awareness rate about clonorchiasis is low among urban residents in Yanqing District. The focus of the future work is to extensively carry out the health education about clonorchiasis prevention and control and take relevant interventions.
6.Quality of Life in Elderly with Cardiovascular Diseases in A Community in Beijing
Ling TIAN ; Qing LIN ; Di CHEN
Chinese Journal of Rehabilitation Theory and Practice 2010;16(5):423-424
ObjectiveTo investigate the quality of life (QOL) of old patients with cardiovascular diseases in community. Methods100 old patients with cardiovascular diseases in Xiluoyuan, Beijing were investigated with WHOQOL-BREF. ResultsThe QOL-BREF scores in the dimensions of physical health, psychology, social relationships, and environment were (53.21±11.50), (53.27±15.99), (54.29±14.41) and (57.65±15.30) respectively. There was not significant difference between self-rating scores and scores in four dimensions of QOL-BREF (P>0.05). There was not significant difference of QOL-BREF scores in four dimensions between male and female, nor the ones whether complicated with diabetes (P>0.05). The incidence of satisfaction was 31% for QOL, 23% for health, and 62% for the healthy system as self-report. ConclusionThe QOL of the patients is related to diseases.
7.Correlation between heel vessel and human balance function.
Hui TIAN ; Di BIAN ; Yuejiao SUI ; Lu REN ; Tieming MA
Chinese Acupuncture & Moxibustion 2015;35(4):352-354
The heel vessel belongs to the eight extra meridians in human meridian system, which is closely related to the human motion function. Balance function plays an essential role in successful completion of activities in daily life, so the physiological function and pathology of heel vessel as well as human balance function are analyzed, and from the aspect of running course of heel vessel and syndrome, the correlation between heel vessel and human balance function is explored, and the application status of acupoints related to heel vessel for balance dysfunction is introduced. It is believed that heel vessel is closely related to human balance function, especially the motion regulation mechanisms of balance function, and it is hoped that new ideas and methods can be provided for acupuncture treatment for the balance function disorders.
Acupuncture Points
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Acupuncture Therapy
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Humans
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Meridians
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Postural Balance
8.Effect of cyclooxygenase-2 on bone loss in ovariectomized rats
Ying GUO ; Chenyan ZHANG ; Ying TIAN ; Jianmin DI ; Shan QIN
Chinese Journal of Obstetrics and Gynecology 2012;47(6):458-462
Objective To investigate mechanism of cyclooxygenase-2 ( COX-2) in bone loss in a postmenopausal osteoporosis (PMOP) rat mode with ovarietomy (OVX).Methods Forty female Sprague Dawley adult rats at age of 3 months were randomly divided into 4 groups,10 in each group,including shamoperated (sham) group,OVX group,OVX treated with nilesteriol (OVX + E) group and OVX treated with aspirin ( OVX + P) group.All rats in OVX,OVX + E and OVX + P groups underwent ovarietomy under abdominal anesthesia with 10% chloral hydrate.Rats in sham group were only taken with fat tissue with same weight under bilateral ovary.After surgery,penicillin was administered to prevent infection.At day 7 after surgery,agents were given by intragastric administration for 12 weeks.Nilestriol at 1.0 mg/kg was used in OVX + E group once a week,aspirin at 45 mg · kg - 1 · d- 1 was used in OVX + P group once a day.Saline with same volume was used in rats in sham and OVX groups.All agents were administered one time per day.Dose of agents were adjusted by weight per week.At end of study,bone mineral density (BMD) of right femurs and lumbar vertebrae 3 -5 (L3-5) were measured.Morphology of bone was detected by hematoxylineosin,and expression of COX-2 was determined by immunohistochemistry staining.Results ( 1 ) BMD:BMD of right femur and L3-5 was (0.209 ±0.010) g/cm2 and (0.230 ±0.012) g/cm2 in sham group and (0.181 ±0.008) g/cm2 and (0.201 ± 0.016) g/cm2 in OVX group,which reached statistical difference (P<0.01).BMD of right femur and L3-5 was (0.203 ±0.009) g/cm2 and (0.224 ±0.028) g/cm2 in OVX + E group and (0.200 ± 0.011 ) g/cm2 and (0.204 ± 0.003 ) g/cm2 in OVX + P group,which were all higher than those in OVX group (P <0.01,P <0.05).However,there was no statistical difference in BMD between OVX + E and OVX + P group ( P > 0.05).(2) Morphology of bone:bone trabeculae became fewer and degenerated in OVX group.However,bone trabeculae were regular and dense in OVX + P group and OVX + E group,which were similar to those in sham group.(3) Expression of COX-2:cells with COX-2 positive and expression of COX-2 around bone trabeculae in OVX group were more than those in sham,OVX + E and OVX + P group.Conclusion COX-2 plays an important role in PMOP.Aspirin could prevent bone loss by decreasing COX-2 expression in OVX rats.
9.Research progress of sorbent hemoperfusion blood compatibility
Di WU ; Tao LI ; Shuaishuai TIAN ; Yichen LI
International Journal of Biomedical Engineering 2014;37(3):179-182
Hemoperfusion is a kind of medical device which contacts directly with blood.In the application,fully considering the interaction between the blood and the sorbent material,so as to improve the blood compatibility is particularly important.With the development of medical science,chemistry and biomedical engineering,a variety of new materials of hemoperfusion have been studied and the blood compatibility of hemoperfusion sorbent has also been greatly improved,making the range of hemoperfusion clinical applications increasingly wide.This paper,combining related research results in recent years,reviews the research progress of the hemoperfusion adsorbent's blood compatibility in recent years.
10.Influence of effective ingredients of Chinese kidney-tonifying drugs on rat bone healing and hemorheology
Di ZHANG ; Zhijie JIA ; Yongli TIAN ; Zhiyu XU
Chinese Journal of Tissue Engineering Research 2011;15(24):4545-4548
BACKGROUND: Chinese kidney-tonifying herb is commonly used in orthopedics department. However, individual effects of different ingredients remain poorly understood. OBJECTIVE: To observe influence of effective ingredients of Chinese kidney-tonifying drugs on bone healing and hemorheology of rats with bone injury. METHODS: Rat model of fracture of shaft of the femur was established, and treated by intragastric administration separately with extractive rhizoma drynariae total flavonoids, epimedium total flavonoids, dodder total flavonoids, naringin, quercetin and hesperidin. Blood and bone samples were harvested after 21 days to detect the extent of bone healing and hemorheology indexes. RESULTS AND CONCLUSION: The effective constituents of different Chinese kidney-tonifying drugs were beneficial to bone healing, and the effect of rhizoma drynariae total flavonoids and epimedium total flavonoids was better than the other drugs. Rhizoma drynariae total flavonoids and epimedium total flavonoids effectively inhibited blood viscosity under low shear, and reduced erythrocyte aggregation index and the aggregation and adhesion of platelet (P < 0.05), but it had no evident effects on erythrocyte deformation. This shows that the effective ingredient of the Chinese kidney-tonifying drugs can promote bone healing and exert certain effects on hemorheology. In particular, rhizoma drynariae total flavonoids exhibits the best effect.