Two isolates of Plasmodium jalcipanm from Hainan Province were cloned by limiting dilution method and eight clones were established. Some characteristics including drug sensitivity and antigenicity of these clones were observed. The results showed that the established clones were different from each other in chloroquine sensitivity and antigenicity. The ID50 of chloroquin3e against 6 clones from isolate Fcc-7801 varied between 60.60 and 13.08 nmol/L The ID50 of chloroquine against 2 clones from isolate Fcc-1 were 93.63 and 49.64 nmol/L, respectively. According to the indirect fluorescent antibody (IFA) Teactivity of these clones with a panal of murine anti-gpl95 McAbs, 8 clones could be divided into 5-serotypes, 3 of which were consistent with the Ⅴ , Ⅵ, Ⅶ serotypes devided by J. S. McBride (1985).

BACKGROUND:How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus.
OBJECTIVE:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells.
METHODS:The cells were divided into two groups:the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione.
RESULTS AND CONCLUSION:At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P<0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P<0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.

Objective To investigate the correlation between drug resistance and β-actamase genes of multi-drug resistance Acinetobacter baumannii (MDR-AB) in neonatal intensive care unit to provide evidence for rational antibiotics administration and nosocomial infection control.Methods Twenty-six MDR-AB strains were separated and collected from clinical specimens.The minimum inhibitory concentrations of 13 antimicrobial agents were determined by agar dilution method.Genotypes of β-lactamase were detected by polymerase chain reaction.Results The resistant rates of the 26 strains to Ceftazidime,Cefoxitin,Piperacillin-tazobactam and Ciprofloxacin were 100.0%.About 80.8% to 96.2% of these strains were resistant to the other antimicrobial drugs.Among the 26 MDR-AB strains,100% (26/26) strains possessed oxa-51,77% (20/26) possessed oxa-23 gene,54% (14/26) carried arnpC gene,both oxa-23 and ampC were identified in 42% (11/26) strains,while oxa-24,oxa-58,imp-1,imp-4 and vim-2 gene were not identified.Conclusions The drug resistance of Acinetobacter baumannii is serious,oxa-23 and ampC are the major plactamase genes carried by MDR-AB in neonatal intensive care unit.

Objective:To study the effect of IL-15 eukaryotic expression plasmid on the humoral immune responses to HBV surface antigen protein vaccine.Methods:Had constructed two of IL-15 eukaryotic expression plasmides.One is the eukaryotic expression plasmid of the IL-15 whole sequence,the other is the chimeric eukaryotic expression plasmid of the IL-2 signal peptide and IL-15 mature peptide sequence(IL-2s-15).The bioactivities of the expression products of the two plasmids were identified by CTLL-2 proliferation assay.Results:After IL-15 plasmid co-immunized with HBsAg,the titer of anti-HBsIgG was much higher than that of control( P 0.05),however,the anti-HBsIgG2a/IgG1 ratio was higher than that of control.Conclusion:IL-15 expression plasmids effect differently on the immune responses induced by protein vaccine.

myocytes.In aging mice induced by D-galactose experiment.TP has anti-apoptosis and anti-oxidation effect in a dose-dependant manner.

OBJECTIVE To re-evaluate Bifidobacterium from microecological point of view. METHODS With the development of microecology,it is paid more and more attention to the medical field.The genesis and development of diseases were closely related with microbe imbalance.As a kind of common microbial population in intestinal tract,Bifidobacterium are one of the most important sources for the exploitation of microecological preparation.Bifidobacterium were re-evaluated by the reference review method. RESULTS This review is focused on the current situation,bioactivity,clinical application,and prospective of Bifidobacterium. CONCLUSIONS Bifidobacterium have good application prospects.

Objective To examine whether hantavirus Z37 envelope glycoprotein genes G1 and G2 recombinants could elicit humoral immune responses in mice. Methods We vaccinated BALB/C mice with plasmid pcDNA3.1 G1 and pcDNA3.1 G2 respectively by intramuscular and gene gun inoculation. Serum anti hantavirus antibodies were detected by IFA and neutralizing antibodies (NAb) were detected by plaque reduction neutralization test with immunoperoxidase staining. The mice given intramuscular inoculation were divided into 3 groups, each group containing 5 mice: groupⅠas control inoculated with 100 ?g pcDNA3.1 ; groupⅡ with 100 ?g pcDNA3.1 G1; group Ⅲ with 100 ?g pcDNA3.1 G2. Each mouse was immunized three times. 4 weeks after primary vaccination, 2 boosts were given at 3 week intervals. The mice given gene gun inoculation were divided into 3 groups, each group containing 3 mice: groupⅠ as control with 1.5 ?g pcDNA3.1; group Ⅱ with 1.5 ?g pcDNA3.1 G1; group Ⅲ with 1.5 ?g pcDNA3.1 G2.Each mouse was immunized two times. 4 weeks after primary vaccination, 1 boost was given. Results 1. In mice given intramuscular inoculation, serum anti hantavirus antibodies were detected 3 of 5 in group Ⅱ, 2 of 5 in group Ⅲ.IFA titers 1∶20～1∶80. NAb were detected 1 of 5 in group Ⅱ, 2 of 5 in group Ⅲ. NAb titers 1∶10～1∶20. 2.In mice given gene gun inoculation, serum anti hantavirus antibodies and NAb were detected in all experimental group mice, IFA titers 1∶20～1∶320, NAb titers≥1∶10. Conclusions Genetic immunization with HV Z37envelope glycoprotein genes G1 and G2 recombinants could elicit effective humoral immune response in mice.

The immune system is a complex system involving multiple organs,and it is vulnerable to age,gender,environment and other factors.For a variation normal physiological range,it is a great challenge to evaluate drug-induced immunotoxicity in preclinical safety study.Histomorphologic assessment of the immune system is a recognized cornerstone in the identification of immunotoxicity at present.In this paper,the principles of pathological evaluation for immune system,and pathological evaluation for important immune organs including thymus,spleen,lymph nodes are discussed briefly,so that it is intended to assist toxicity pathologists in the accurate and consistent characterization of intended and unintended drug-induced alterations of the immune system.

Objective To investigate the immunologic classification in the patients with acute leukemia (AL) in Xinjiang of China. Methods A panel of monoclonal antibodies (MOAb) and indirect immunofluorescence assay by fluoromicroscope was used to determine the pretherapy immunophenotype of 450 AL. Results 106 cases of acute lymphoblastic leukemia (ALL), 334 cases of acute myelogenous leukemia (AML), and 10 cases belonged to FAB unclassified acute leukemia (UAL) were unalysed. The expression of myeloid antigens in of ALL was seen in 15 % of 106 cases, and lymphoid-associated antigens were expressed in 25 % of 334 AML cases. The most frequently expressed antigen was CD7. The expression of myeloperoxidase (MPO) gene in 295 cases of AL were studied. The expression of MPO gene was observed in positive one of 81 ALL cases, and myeloid cells had different expression for MPO gene. Of the 9 cases of UAL, 6 cases were positive for MPO gene. There were no statistic differences of the expressions of the ALL stages between Han and Wei nationality. The order of myeloid markers expression in AML was as follows: CD_(33)>CD_(13)>CD_(15) inthe Han nationality, and the order of myeloid markers expression in AML was displayed CD(15)>CD(33)>CD_(14) in Wei nationality. Conclusion Analysis of immunophenotype assured accurate lineage diagnosis of AL. Combinatively analyzing the characteristics of AL on morphology, cytochemistry, immunology and MPO mRNA expressions were significant to the diagnosis and therapy of AL.

Objective To study the recovery of the peripheral lymphocyte subsets in patients underwent allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and guide the prevention and treatment of infection. Methods Indirect immunofluorescence assay was used to detect the lymphocyte subsets, such as T cell subsets (CD3, CD4, CD8). B cell (CD19) and natural killer cell(CD56) at 1, 3, 6, 12, 18months post transplantation, in the meantime, lymphocyte subsets of 32 samples from healthy blood donors were tested as normal control values. Results CD+3, CD+4 and CD+8 ceils significantly decreased than that of normal control at 1 month post transplantation, the recovery of CD+3 T cells was within 3-12 months, CD+4 and CD+8 T cells recovered to normal at 6 months and 3 months post transplantation respectively, CD+4/CD+8 ratio were not significantly lower than that of normal control at different stages, CD+4/CD+8 ratio reversed only at 6 months post transplantation. CD+19 and CD +56 T cells recovered quickly and they were more than normal proportion at 3 months post transplantation. The CD+3, CD+8 T cells and CD+4/CD+8 ratio were statistically higher in HLA haploidentical allo-PBSCT patients than that in HLA identical allo-PBSCT at 3 months post transplantation. There were no difference between the two groups at 1, 6, 12, 18 months post transplantation.The patients with cGVHD had significantly higher CD+4 cells than those without cGVHD at 1 month after transplantation. There was no significant difference in all of the lymphocyte subsets at 3, 6, 12, 18 months after transplantation between them. Conclusion Allo-PBSCT has a hastened immune reconstitution, which was not delayed by the incompatibility of HLA and the development of cGVHD.