BACKGROUND:With the continuous development and progress of medical model, the treatment and rehabilitation recovery from peripheral nerve defects needs higher requirements; therefore, stem cel culture-based nerve tissue engineering technology provides a new strategy for nerve defect repair. OBJECTIVE:To explore the feasibility of combining bone marrow mesenchymal stem cels and new nerve conduits to repair peripheral nerve defects. METHODS:Fifty New Zealand white rabbits, clean grade, were randomly divided into experimental group and control group, with 25 rabbits in each group. Animal models of peripheral nerve defects were made about 15 mm distant to the middle segment of the radius of the rabbit foreleg. At 1 week after modeling, bone marrow mesenchymal stem cels/new nerve conduit composite material was implanted into the defect in the experimental group; and in the control group, only bone marrow mesenchymal stem cels were transplantation. At 4 weeks after transplantation, a 5-mm nerve fiber was taken from the defect site, stained with hematoxylin-eosin and observed under scanning electron microscope. Density and diameter of regenerated nerve fibers were compared between the two groups. RESULTS AND CONCLUSION:Compared with the control group, the density of nerve fibers was significantly higher in the experimental group, but the diameter of nerve fibers was significantly lower (P < 0.05). There were more cels with good growth, large size and many processes on the surface of regenerated nerve tissues in the experimental group than the control group. Moreover, in the experimental group, interconnected cels were woven into a mesh and the axons were longer and thicker, both of which were the performance of typical neuron-like cels. Taken together, the bone marrow mesenchymal stem cels/new nerve conduit composite material can be used for peripheral nerve repair and present exact achievements.

ObjectiveTo study the effect of different treatment on prolapse of lumbar intervertebral disc. Methods 169 patients with prolapse of lumbar intervertebral disc were divided into two groups, the contral group treated with traction and massage, and the comprehensive group treated with acupuncture, physical therapy, and phsiotherapy besides traction and massage. The assessment included index and rate in improvement and efficiency of rehabilitation. Results The index and rate in improvement and efficiency of rehabilitation is better in the comprehensive group than in the contral group. The course of treatment in the comprehensive group is shorter than that in the contral group.

Objective To study the change of Th1, Th2 cytokine levels in culture supernatants of PBMC in pregnancy induced hypertension (PIH). Methods 43 PIH patients, 15 normal pregnant women, and 15 healthy non-pregnant women were studied randomly. Levels of IL-2?IFN? and IL-4 in culture supernatants of PBMC were detected by ELISA. Results In culture supernatants of PBMC, IL-2 level of normal pregnant women was(140.3? 73.2 )ng/L, decreased significantly in comparation with that of healthy non-pregnant women (259.5?114.4)ng/L, P

OBJECTIVE:To provide reference for rational drug use in the clinic. METHODS:The calculation was carried out in type,amount and consumption sum of Chinese patent medicines containing Salvia miltiorrhiza in our hospital during 2011-2014. These data were analyzed,classified and sorted by DDDs sequence and the method of pharma-coeconomics with Excel statistically. RESULTS:The consumption sum of Chinese patent medicines containing S. miltiorrhiza took on little changes in our hospital dur-ing 2011-2014,decreasing by 10.52% in 2012 compared to 2011 but increasing by 8.49% in 2013 compared to 2012. The order of DDDs kept stable,indicating that Chinese patent medicines containing S. miltiorrhiza were used frequently in 4 year. The order of consumption sum also kept stable relatively. CONCLUSIONS:The utilization of Chinese patent medicines containing S. miltiorrhi-za in our hospital is rational. Those with suitable prices and proved efficacy take a great share in the clinical application.

Objective To Observe the effects of tumor necrosis factor ? (TNF ?) on cell proliferation and Intracellular free calcium concentraction ([Ca 2+ ]i) in endothelium of human umbilical vein endothelial cells (HUVEC) and investigate the pathogenesis of pregnancy induced hypertension syndrome (PIH). Methods Confluent monolayer of HUVEC was directly incubated with TNF ? at following final concentrations: 500, 1 000, 2 000 U/ml for 24 hours. The percentages of different cellcycles and [Ca 2+ ]i were measured by flow cytometry and fluorospectrophotometry. Results Incubated with TNF ?, the endothelial cells were elongated and transformed into fibroblast like cells. Border of nucleus was sharp, clarity, and cells were in regular shape. But there were abnormal granules in cytoplasma and some cells detached at the concentrotion of 2 000 U/ml of TNF ?. Stimulated by TNF ?, the percentage of cellcycles from phase G 0+G 1 to S and G 2+M decreased significantly and it was dose dependent. [Ca 2+ ]i increased significantly and dose dependent as well. Conclusion TNF ? may injure endothelium directly and inhibit its proliferation and repair through the changes of [Ca 2+ ]i level. It may play an important role in the pathogenesis of PIH.

Objective To prepare the Pt/CeO2/GAC catalyst used to catalyze oxidation of condensate wastewater and to investigate whether the doping of Ce can increase catalytic activity for oxidizing condensate wastewater.Methods Impregnation glycol reduction method was adopted to prepare Pt/CeO2/GAC catalyst,and it was characterized by BET,XPS and XRD.The catalytic activity was determined by batch experiment.Results The experimental results revealed that more than 93.4% of ethanol in the condensate wastewater was oxidized to aldehyde and acetic acid with 13.33 g?L-1 Pt/CeO2/GAC catalyst dose,for 2 h reaction time,at 40 ℃ and normal pressure.In contrast,the oxidation efficiency was only 85.0% with Pt/GAC catalyst without doping Ce in the same reaction condition.Conclusion It is proven that the doping of Ce in Pt/GAC improves the catalytic activity.The characterization of Pt/CeO2/GAC catalyst shows that Ce is presented as Ce4+ that not only increases the content of chemisorbed oxygen on the surface of the catalyst,but also increases the content of Pt greatly.

OBJECTIVE: To provide references Chinese medical institutions about the composing of formulary. METHODS: We reviewed online document and literatures, briefly introduced the concept, structure and contents of formulary as well as the management process of the formulary system in foreign countries. RESULTS & CONCLUSIONS: Formulary can provide effective information in the health-care settings, and the information related to formulary and formulary system abroad serves as a mirror for the composing and enforcing of formulary in China.

Objective To explore the clinical characteristics of the patients with schizophrenia to make troubles and accidents likely ,to provide scientific evidence for preventing and controlling these patients to make troubles and accidents effectively .Methods 100 patients with schizophrenia to make troubles and accidents likely were randomly extracted and assigned to the study group from psychiatric department of this hospital .100 ordinary patients were divided into control group .Their data on illness were sur-veyed and analyzed retrospectively .The difference of the general condition and illness data along with mental symptoms between the both group were compared .Results The proportion of patients to be man peasant ,in rural area ,no a spouse and medical insurance , to have a lower degree of culture and to be 20 - 39 years old in the study group was significantly higher than those in the control group(P< 0 .05) .The social support and economic conditions of family in the study group were significantly poorer than those in the control group(P< 0 .05) .The proportion of patients to be paranoid schizophrenia ,to have a complications and the positive score of the positive and negative syndrome scale (PANSS) in the study group were significant higher than those in the control group but the negative score of PANSS and the course of disease were significantly lower (P< 0 .05) .The proportion of patients to have posi-tive symptoms such as illusion and delusion in the study group were significantly higher than that in the control group (P< 0 .05) . But the proportion of patients to have negative symptoms such as avoid social and emotional dull in the study group were signifi -cantly lower than that in the control group(P< 0 .05) .Conclusion The positive symptoms contribute to making troubles and acci-dents in SP patients .The patients to be paranoid schizophrenia ,a man and peasant ,in prime of life ,in rural area ,no a spouse and to have a lower degree of culture maybe make troubles and accidents more likely .Having a poor social support and economic conditions of family and no medical insurance cause the probability of their making troubles and accidents increase .

Objective To investigate the effects of cell hypoxia on radio-inducible activity of early growth response gene-1 (Egr-1) promoter and its mechanisms. Methods The reporter vector of Egr-1 promoter was constructed by inserting the promoter sequence upstream the lucifearase gene in pGL3, which was then transfected into the lung adenocarcinoma A549 cell by liposome. The luciferase activity and H 2O 2 production were detected in the transfected cells exposed to doses of 2, 4, 6, 8, and 10 Gy radiation combined with the oxygen concentrations of 0.1%, 0.5%, 1%, 2.5%, and 5 %. The changes of luciferase activity in the transfected cells exposed to various concentrations of H 2O 2 were observed. Results The reporter vector of Egr-1 promoter was constructed successfully. The radio-inducible activity of Egr-1 promoter decreased significantly in the transfected cells exposed to oxygen concentration below 2.5% in a concentration-dependent manner, as compared with that in the normal control (P

Objective To develop a sensitive, simple, and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract (RAPE). Methods The separation was achieved by HPLC on a RP18 column (150 mm × 3.9 mm, 5μm) with a mobile phase composed of acetotitrile-0.05% phosphoric acid water (98: 2) at a flow rate of 1.0 mL/min. UV Detector was set at 200 nm and friedelin was chosen as an internal standard. Results The linear range of the standard curves was (0.3443-22.0) μg/mL with the correlation coefficient of 0.9968. The intra- and inter-day precisions were all below 10% and the relative error was -3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study. After ig administration of RAPE, T1/2(ka) is (33.09 ± 7.32) min and T1/2(ke) is (84.95 ± 22.34) min.