The concentrations of serum lipids, lipoproteins and apoprotein AI, B100, CⅡ and CⅢ are determined in 32 obese children and age- and sex-matched healthy children. This study aims: 1. to know whether obesity can affect the levels of serum lipids and apoproteins. 2. to understand the correlation between degree of obesity and serum lipids and apoproteins. 3. to study the mechanism of change of serum lipids. In obese subjects, the levels of HDL-C, APO-AI, HDL-C/TC and APO-CⅡ/CⅢ decreased significantly and are negatively correlated to degree of obesity. The levels of TG, VLDL-C, APO-B100, APO-CⅡ and atherosclerosis index increased significantly and are positively correlated to degree of obesity. The levels of cholesterol, LDL-C and APO-CⅡ revealed no change. Multiple correlation analysis showed that TSFT was closely related to HDL-C, STR was closely related to VLDL and APO-C Ⅱ/CⅢ. The mechanism of change of serum lipids and apoprotein is disscused. These results suggest: 1) Obesity can affect the levels of serum lipids and apoproteins. 2) The change of apoprotein and regional fat distribution is a major cause of change of serum lipids. 3) Obese children are at risk to atherosclerosis.

Objective To study the effect of Thrombolytic Compound (TC) injected into internal carotid at superacute stage.Methods 66 Wistar rats were divided into group A,B and C randomly. Focal cerebral ischemic model was established by improved microthromboembolus method. The rats were intubated conversely into internal carotid 3h after cerebral ischemia for treatment with normal saline (group A),Urokinase(group B) and TC(group C) respectively. The neural functions of these rats were evaluated at 2 h after cerebral ischemia,2.5 and 23.5h after treatment and pathological changes were observed under optic and electron microscopes 24 h following thrombolysis.Results There was no significant difference of neural function scores among the three groups before administration.The scores of group C were lower than those of group A after treatment ( P

Purpose　The aim is to find out an effective preventing influenza immune preparation. Methods　Egg yolk immune solution of anti-influenza virus(FM1 stem) was prepared from egg of the hens that had been successfully immunized with influenza virus (FM1 stem). Its effects of anti-virus were observed through animal experiment.Results　When the mice of control group began to die the average daily drink quantity of the mice of normal saline control group, egg yolk immune solution control group, immediate preventive group was (3.06±0.86), (2.93±1.47) and (3.99±0.21)ml(P＜0.05)respectively. The average body weights of the mice of these three groups were (15.85±2.70),(14.58±1.92) and (18.27±1.71)g(P＜0.05)respectively. Their mortality was separately 53.84%,69.23% and 3.84%(P＜0.01). The antibody positive rate of survived mice′s serum (1∶10 diluted) was separately 100%, 100% and 0%(P＜0.01). Conclusion　The anti-virus effects of egg yolk immune solution of anti-influenza virus was powerful. The result of preventing mice′s influenza was remarkable.

Objective To study the survival status of very elderly patients with intertrochanteric fracture admitted in hospital and its risk factors.Methods Clinical data of 54 patients with intertrochanteric fracture aged 90 years and over admitted in hospital from Jan.2006 to Dec.2013 were retrospectively analyzed.5 patients were lost to follow-up.The survival status of patients was analyzed.Results Patients were followed up until January 2015.The period of follow-up ranged from 85 months to 0.2 months,with an average follow-up of 33.3 months.17 cases were survived.Harris scores were(61.2 ± 10.6).The overall 1-,3-,5-year survival rates were(0.88 ± 0.05),(0.35 ±0.08),(0.18 ± 0.08),respectively.The mean and median survival times were (33.3 ± 4.6) months and(25.0±5.4) months.Cox proportional hazard regression model showed that American Society of Anaesthesiologists(ASA) score,operation time and the number of comorbidities were the risk factors for the survival time of very elderly patients with intertrochanteric fracture (all P ＜0.05).Conclusions Compared with conservative treatment,surgical treatment can increase the survival time of the elderly patients with intertrochanteric fracture.ASA score,operation time and the number of comorbidities may be the risk factors for the survival time of very elderly patients with intertrochanteric fracture.

Thymopentin (TP5) and bursopentin (BP5) are both immunopotentiators. To explore whether the TP5-BP5 fusion peptide (TBP5) has adjuvant activity or not, we cloned the TBP5 gene and confirmed that the TBP5 gene in a recombinant prokaryotic expression plasmid was successfully expressed in Escherichia coli BL21. TBP5 significantly promoted the proliferation of thymic and splenic lymphocytes of mice. The potential adjuvant activity of the TBP5 was examined in mice by coinjecting TBP5 and H9N2 avian influenza virus (AIV) inactivated vaccine. HI antibody titers, HA antibodies and cytokines levels (IL-4 and IFN-γ) were determined. We found that TBP5 markedly elevated serum HI titers and HA antibody levels, induced the secretion of both IL-4 and IFN-γ cytokines. Furthermore, virus challenge experiments confirmed that TBP5 contributed to inhibition replication of the virus [H9N2 AIV (A/chicken/Jiangsu/NJ07/05)] from mouse lungs. Altogether, these findings suggest that TBP5 may be an effective adjuvant for avian vaccine and that this study provides a reference for further research on new vaccine adjuvants.
Adjuvants, Immunologic ; pharmacology ; Animals ; Antibodies, Viral ; blood ; Cell Proliferation ; drug effects ; Influenza A Virus, H9N2 Subtype ; drug effects ; physiology ; Influenza Vaccines ; immunology ; Interferon-gamma ; immunology ; Interleukin-4 ; immunology ; Lymphocytes ; drug effects ; Mice ; Oligopeptides ; immunology ; Orthomyxoviridae Infections ; drug therapy ; Recombinant Fusion Proteins ; immunology ; Spleen ; cytology ; Thymopentin ; immunology ; Thymus Gland ; cytology ; Vaccines, Inactivated ; immunology ; Virus Replication

Objective To investigate the effect of infiltrated mast cells on the biological characteristics of uterine carcinoma.Methods Twelve leiomyomas,ten uncertain malignant uterine leiomyomas,and seven uterine leiomyosarcomas were studied with light microscopy for the morphometry about their histological feature,mast cell particle and factor Ⅷ-related antigen(FⅧRAg);Cell apoptosis was measured by flow cytometry.The correlative analysis was carried out between the mast cell and others factors.Results Comparing with smooth muscle tumors of uncertain malignant potential(4209.9?273.0),the count of tumor cell was the highest in leiomyosarcoma(3557.6?346.3)(P

Objective The purpose of this paper was to use a new biphasic poroelastic tibia model to develop a two-dimensional numerical method for simulating impact responses of human tibia in car-pedestrian accidents. Methods The geometry of tibia model was reconstructed from CT scans of the left tibia of a living human volunteer. A poroelastic approach was utilized to establish the governing equations of the model and the finite element method was applied to solve these governing equations. Both cortical and cancellous components of tibia were represented using a poroelastic material model consisting of solid phase (matrix) and fluid phase (marrow). A lateral-medial impact direction was selected in the simulation analysis and the impact responses of the pedestrian tibia during 0-200 ms were analyzed. Results The bending deformation of the tibia predicted by the computer simulation was primarily concentrated on the impact zones. The displacement response of Node 107 in the impact zone indicated a peak displacement of -6 mm at around 75 ms, and the significant time delay between the impact force and the displacement response of the skeleton. The axial stress response at the center of element E77 in the impact zone indicated a peak stress of 140 MPa at around 30 ms,and the significant time delay was observed between the impact force and the axial stress response of the skeleton, too. Conclusion This research developed a two-dimensional numerical method for simulating impact responses of human tibia in car-pedestrian accidents. It was able to approximately simulate the bending deformation, lateral displacement response and axial stress response of pedestrian tibia in the impact zones,and the effects of the fluid phase on the solid phase. More in-depth investigation is helpful to further the biofidelity of tibia dynamics model.

A biphasic porous medium model based on the mixture theory in continuum mechanics frame was used to depict the distributions of osseous stress field, distortion field and pore pressure when the bone tissue was subject to various dynamic loads. In the model, the bone tissue was considered as a transversely isotropic, liquid saturated porous material. The coupling relationship among the distortion, fluid flow and the streaming potential is studied. The Galerkin weighted residual method was used to derive the finite element formulation for dynamic response and the streaming potential calculating formulation of bone tissue, the penalty finite element formulation was obtained via introducing the ratio term of pressure p and penalty parameter beta in the continuity equation and, in turn, eliminated the pressure term in governing equation sets. The computational results show that the viscoelastic behavior and the energy dissipation property in the bone tissue, especially in the cancellous bone, is caused to a great extent by the pore liquid flow and diffusion. Meanwhile, because of the existence of electrical double layer between the solid phase and liquid phase, when the liquid constituents in the pore diffuse, the streaming potential appears, and the growth and absorption of bone tissue is expedited.
Biomechanical Phenomena ; Biophysical Phenomena ; Biophysics ; Bone and Bones ; physiology ; Elasticity ; Electrophysiology ; Finite Element Analysis ; Humans ; Models, Biological ; Porosity ; Stress, Mechanical ; Viscosity

Breast cancer is the most common cancer among women worldwide, and it seriously threatens women′s life and health. circular RNA (circRNA) play a key role in the development and drug resistance of various cancers, including breast cancer. circRNA is an endogenous RNA molecule with a single-stranded closed structure, which has unique biological characteristics and function, and is a current research hotspot. This article will review circRNA as potential biomarkers to predict breast cancer drug resistance and therapeutic targets.