1. Clinical characteristics and prognosis of 34 cases of acute myeloid leukemia with FLT3 internal tandem duplication and MLL gene rearrangement
Jiarui ZHOU ; Xian ZHANG ; Yanli ZHAO ; Junfang YANG ; Jianping ZHANG ; Xinyu CAO ; Yue LU ; Deyan LIU ; Fanyong LYU ; Jian OUYANG ; Peihua LU
Chinese Journal of Hematology 2018;39(9):751-756
Objective:
To analyze the clinical characteristics and prognosis of 34 cases of acute myeloid leukemia (AML) with FLT3 internal tandem duplication (FLT3-ITD) and MLL gene rearrangement.
Methods:
The clinical data of 34 AML patients with FLT3-ITD and MLL gene rearrangement was compared and analyzed for the therapeutic efficacy, prognostic factors when treated with chemotherapy, chemotherapy combined with targeted therapy or allogenic hematopoietic stem cell transplantation (allo-HSCT).
Results:
Of the thirty-four cases with median age 41 (4-71) years old, 63.6% presented with white blood cells (WBC) greater than 30×109/L, 39.4% greater than 50 × 109/L respectively on admission. M5 (35.3%) made up the highest proportion. The cytogenetic abnormality reached 61.8%, of which the complex cytogenetic abnormality accounted for 11.8%. Eleven patients (32.35%) had both FLT3-ITD and MLL gene abnormalities. In addition to FLT3 and MLL abnormalities, 23 patients (67.6%) had one or more other gene abnormalities (multiple gene abnormalities). Of the 34 cases, 29.4% patients went into complete remission (CR) after two courses of chemotherapy. 20.6% (7 patients) went into CR after 3 or more courses of chemotherapy. The rate of early relapse in the CR group was 52.9%. Patients with WBC>50×109/L or multiple gene abnormalities had a lower remission rate (7.7%, 5.4%) after two courses of chemotherapy. CR rate for the patients with more than three gene abnormalities was 0. The total 2-year overall survival (OS) in the 34 patients was 28.8% (95%
2. Effect of minimal residual disease monitoring by multiparameter flow cytometry pre-conditioning on prognosis of acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation
Yue LU ; Tong WU ; Hui WANG ; Yanli ZHAO ; Xingyu CAO ; Deyan LIU ; Jianping ZHANG ; Min XIONG ; Jiarui ZHOU ; Ruijuan SUN ; Zhijie WEI ; Shuquan JI ; Daopei LU
Chinese Journal of Hematology 2017;38(2):118-123
Objective:
To investigate the effect of minimal residual disease (MRD) monitoring by multiparameter flow cytometry (MFC) pre-conditioning on prognosis of acute myeloid leukemia in first complete remission (CR1-AML) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) , and to explore the value of MRD monitoring by MFC in the prognosis evaluation on allo-HSCT in CR1-AML.
Methods:
Between April 2012 and March 2015, consecutive 186 patients with CR1-AML who underwent allo-HSCT were analyzed retrospectively. MRD in BM before conditioning was detected by eight-color MFC. Any level of residual disease was considered to be MRD positive.
Results:
①Of 186 patients, MRD was negative in 151 patients, positive in 35 patients (<1% in 25 patients and 1% to 3% in 10 patients) . ② With the median follow up of 18 (5-41) months, two-year DFS was 80.0% (95%
3. Comparison of three different types of donor hematopoietic stem cell transplantation for intermediate and high-risk myelodysplastic syndrome
Yue LU ; Tong WU ; Yanli ZHAO ; Xingyu CAO ; Deyan LIU ; Min XIONG ; Jiarui ZHOU ; Jianping ZHANG ; Zhijie WEI ; Ruijuan SUN ; Daopei LU
Chinese Journal of Hematology 2017;38(4):301-306
Objective:
To investigate three different types of donor hematopoietic stem cell transplantation (HSCT) for intermediate and high-risk myelodysplastic syndrome (MDS) .
Methods:
Between August 2001 and May 2015, 167 consecutive patients with MDS in intermediate and high-risk who underwent allogeneic HSCT were analyzed retrospectively.
Results:
With the median follow up of 60 (12-177) months, The total 5-year DFS was 67.8% (95%
4.HLA- haploidentical donor hematopoietic transplantation for severe aplastic anemia achieved comparable outcomes with HLA- unrelated donor transplantation.
Yue LU ; Tong WU ; Xingyu CAO ; Yanli ZHAO ; Deyan LIU ; Ruijuan SUN ; Min XIONG ; Zhijie WEI ; Jianping ZHANG ; Jiarui ZHOU ; Daopei LU
Chinese Journal of Hematology 2016;37(1):35-38
OBJECTIVETo evaluate the efficacy of HLA- haploidentical donor hematopoietic transplantation (Haplo- HSCT)for severe aplastic anemia (SAA)by compared with the same period of unrelated donor transplantation (UD- HSCT).
METHODSOf a cohort of 50 SAA patients between September 2012 and July 2014, 26 patients underwent UD- HSCT and 24 patients Haplo- HSCT.
RESULTSOS rate was 91.3% with a median follow-up of 9 (2-26)months. According to transplant type, there was no significant difference between UD- and Haplo-HSCT (96.1%vs 86.0%,P=0.30). 3 of 50 (6%)patients had primary engraft failure. Haplo- HSCT developed higher significantly incidence of Ⅱ- Ⅳ aGVHD (37.5%vs 3.83%,P=0.003)and cGVHD (37.5%vs 15.3%,P=0.030)than UD-HSCT. Haplo-HSCT also had significantly higher incidences of CMV viremia (78.2%vs 46.1%,P=0.005)and EBV viremia (43.1%vs 16.0%,P=0.040), respectively than UD-HSCT. But the incidences of hemorrhagic cystitis were similar between two transplant types (39.1%vs 23.0%,P=0.120).
CONCLUSIONThis study showed favorable outcome of Haplo-HSCT for SAA, which was comparable with UD-HSCT.
Anemia, Aplastic ; therapy ; Hematopoietic Stem Cell Transplantation ; Humans ; Incidence ; Treatment Outcome ; Unrelated Donors
5. Effect of NCCN (2015) risk stratification on prognosis of patients with acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation
Yue LU ; Tong WU ; Yanli ZHAO ; Xingyu CAO ; Deyan LIU ; Jianping ZHANG ; Min XIONG ; Jiarui ZHOU ; Ruijuan SUN ; Zhijie WEI ; Hui WANG ; Hongxing LIU ; Tong WANG ; Chunrong TONG ; Shuquan JI ; Daopei LU
Chinese Journal of Hematology 2017;38(1):44-49
Objective:
To analyze the effect of NCCN (2015) risk stratification on prognosis of patients with acute myeloid leukemia (AML) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) .
Methods:
Retrospective analysis of 258 patients with AML in CR (186 cases in CR1, 72 cases in CR2) who underwent allogeneic HSCT in our hospital between April 2012 and March 2015 according to NCCN (2015) risk stratification. Of them, 63 cases were classified as low risk, 112 cases intermediate risk and 83 cases high risk.
Results:
①With the median follow up of 18 (5-41) months, two-year disease free surviva (DFS) in 258 patients was 78.0% (95%
6. Role definition in nursing handoff: a focus group study
Xiaoyun WANG ; Dianhui WANG ; Guomei YE ; Deyan CAO ; Xia CUI ; Feifei CHEN ; Xingfeng LIN
Chinese Journal of Practical Nursing 2019;35(33):2617-2620
Objective:
To identify the impeding and promoting factors of role definition in nursing handoff, in order to increase the quality of nursing handoff.
Methods:
Two nursing managers, 22 head nurses and four nurses were recruited by purpose sampling. Data were collected by using focus group study, and analyzed using content analysis.
Results:
According to the framework of role transition theory, the factors could be classified as individual factors (37 items, 23.72%), organizational factors (105 items, 67.31%), effectiveness of role definition (11 items, 7.05%), and problems of role definition (3 items, 1.92%).
Conclusions
Role definition in nursing handoff helping the coming nurse check the performance of the last shift and transfer the nursing responsibility, contributes to the improvement of nursing quality, but there are still many factors affecting the role definition in nursing handoff.
7. The validation of the Chinese version of Handoff Clinical Examination Exercise
Feifei CHEN ; Chunmei WANG ; Xiaomin WANG ; Deyan CAO ; Guomei YE ; Xiaoyun WANG ; Xingfeng LIN
Chinese Journal of Practical Nursing 2020;36(6):462-466
Objective:
To test the reliability and validity of the Chinese version of the Handoff Clinical Examination Exercise (Handoff CEX).
Methods:
The Chinese version of the Handoff CEX was composed of two parts, one to assess the nurse providing the handoff and another to assess the nurse receiving the handoff. A total of 80 handoffs was evaluated to test the reliability and validity of the Chinese version of the Handoff CEX.
Results:
A total of 320 evaluations of handoff was obtained. The Cronbach α coefficient was 0.85 for the handoff provider, and that was 0.80 for the handoff recipient. Weighted kappa scores for provider evaluations ranged from 0.31-0.52, and that for recipient evaluations ranged from 0.35-0.55. The correlation coefficients between each factor ranged from 0.23 to 0.62, and that between each factor and the total scale ranged from 0.33 to 0.64 (