Objective:To investigate the methylation status of O6-methylguanine-DNA methyltransferase (MGMT) in meningioma tissue and its effect on the growth and metastasis of human meningioma cell line IOMM-Lee cells.Methods:The specimens of 34 patients with meningioma were collected. Methylation-specific polymerase chain reaction (MSP) method was used to detect MGMT methylation in meningioma tissue and normal brain tissue; immunohistochemical staining was used to detect the expression of MGMT in meningioma tissue and normal brain tissue. The cultured human glioma cell line IOMM-Lee cells were divided into blank control group (normal cultured IOMM-Lee cells), negative control group (empty vector virus transfected IOMM-Lee cells) and RNAi lentivirus transfection group (transfected with RNAi lentivirus vector to down-regulate the expression of MGMT). The expression of MGMT in IOMM-Lee cells was silenced by RNAi technology. The expression levels of MGMT mRNA and protein in cells were detected by real-time fluorescence quantitative PCR and Western blot. The proliferation activity of cells was detected by cell counting kit-8 (CCK-8) test, the colony forming ability was detected by cell clone formation test, and the invasion and migration ability of cells in each group were detected by Transwell test and cell scratch test.Results:The methylation of MGMT in meningioma tissue reached 88.23% (30/34). MGMT methylation was not detected in normal brain tissue; the staining intensity of MGMT in meningioma tissue was significantly higher than that in normal brain tissue. Compared with the blank control group and the negative control group, the relative expression of MGMT mRNA and protein in IOMM-Lee cells of the RNAi lentiviral transfection group were significantly decreased (all P<0.05). After 24, 48 and 72 hours of transfection, the proliferation activity of IOMM-Lee cells decreased significantly (all P<0.05), with reduced number of cell clone formation and cell invasion (all P<0.05). The rate of scar healing decreased significantly ( P<0.05). Conclusions:MGMT is mostly hypermethylated in human meningiomas. Silencing the expression of MGMT in meningiomas can inhibit the growth and metastasis of meningiomas.

Objective To analysis the electroencephalographical (EEG)specificity of psychological adjustment of Imaginary Working (IW)by comparing the EEG power of trained subjects and untrained subjects.Methods University students were recruited as the subjects,randomly and equally divided into experimental group and control group.The students of experimental group were trained Imaginary Working,with no intervention on those of control group.The changes of powers during the process of Imaginary Working were synchronously detected by EEG,furthermore,the delta(δ)band,theta(θ) band,alpha(α)band,and beta (β)band were compared and analyzed from three aspects of general points,time interval,and different leads.Results There were very significant differences in δ,θ,αand βbands between the two groups at both Imaginary Working (IW)and resting state (RS)(P <0.001):the absolute power of δband in experimental group was lower than that of control group,and the absolute powers of θ,αand βbands were higher than those of the control group.There were significant differences of absolute power of δband at RS0 ,IW1 ,IW2 and RS3 (P <0.05),and there were significant differences of θband at RS 1 ,RS2 and IW3 (P <0.01 ).The changes of absolute power of δand θbands in experimental group were close to cosine curves.Although there were statistical differences of αband and βband at each state,from IW1 period the amplitude of αband began to keep stable,and from IW2 period the amplitude of βband did.There were no significant differences of δband and θband appeared different leads between the two group(P >0.05).The absolute power of αband showed statis-tical differences in lead of F7,C3,C4,P3,P4,T3,T4,T5,T6,PZ,O1 and O2(P <0.05),while that of βband showed significant difference in lead of FP2,F7,F8,P3,P4,T5,T6,C4,PZ,O1 and O2 (P <0.05).The highest power of αand βband both appeared in the lead of O1 and O2.Conclusion Imag-inary Working induced the rhythmic response of δ,θ,αand βband,especially the rhythm alternated fast waves and slow waves.It is suggested that Imaginary Working keep the brain in rest state,and relieve tension,and adjust negative emotions.

Objective To analyze the effects of different kinds of Image Thinking on brain functional area by measuring alpha band electroencephalograph (EEG) power.Methods 48 college students were enrolled and divided into two groups .Subjects in the experiment group received three kinds of Image Thinking intervention , while those in the control group did not receive any intervention .Alpha band ab-solute power of two groups was measured to compare and analyze .Results The alpha band absolute power value of the experiment group was higher than that in the control group at occipital lobe , parietal lobe , and temporal lobe in both active and resting state ( P<0 .05 ) .The power in αfrequency range was significantly different in three kinds of Image Thinking status.When imagining“I am rambling in the rose bushes”, there was statistically significant difference in contralateral lead in ( FP1-FP2 ) , ( F7-F8 ) , (C3-C4), (P3-P4), (T5-T6), and (O1-O2) (P<0.05).Moreover, power in the right hemisphere was higher than left hemisphere , and after active stage the power of occipital lobe reduced .When imagi-ning “the spring sunshine is warm on my body”“My every pore is breathing freely”, there was statistically significant difference in contralateral lead in (F7-F8) and (P3-P4) (P<0.05).The alpha band abso-lute power increased at occipital lobe after active stage , then the power increased to peak at final resting state.Conclusion Image thinking exhibits a significant tranquilizing effect according to the reflection of the high power of alpha band at occipital lobe and parietal lobe .Different kinds of feeling effect caused by Image Thinking maybe related to the function of every brain area .

Objective:To investigate the role of miR-145 in inflammatory response and immune regulation after traumatic brain injury(TBI).Methods:Male C57BL/6 mice were randomly divided into sham operation group(Sham),model group(TBI),TBI+NC agomir group and TBI+miR-145 agomir group.Modified Nerve Injury Severity Score(mNSS)was used to evaluate neurological function after trauma.MWM test was used toevaluates neurocognitive function of mice after TBI.Flow cytometry was used to detect the number of Tregs in tbrain tissue of each group of mice.ELISA was used to detect expressions of inflammatory cytokines in hippocampus of each group of mice.Immunohistochemistry was used to detect expression of activated microglia/macrophage Iba-1 in hippocampus of each group of mice.RT-qPCR was used to detect expressions of M1/M2 microglia/macrophage marker genes iNOS,CD11b,CD206 and Arg1.TUNEL staining and neuronal nuclear immunity double staining with fluorescent label(NeuN)were used to detect neuronal apoptosis.Results:Compared with Sham group,expression of miR-145 in hippocampus of mice in TBI group was significantly decreased,the neurological damage was increased,and percentage of Tregs in CD4+T cell population in brain tissue was decreased.Expression levels of IL-1β,IL-6,TNF-α,IL-4,IL-10 and TGF-β in hippocampus were significantly increased,the number of activated microglia/macrophage IBA-1 was increased,expression levels of iNOS CD11b,CD206 and Arg1 were significantly increased,and the neuronal apoptosis was increased.Notch1,p21 and Hes1 mRNA and protein levels were significantly increased(all P<0.05).Compared with TBI+NC agomir group,expression of miR-145 in hippocampus of mice in TBI+miR-145 agomir group was significantly increased,and neurological damage was reduced.Percentage of Tregs in CD4+T cell population in brain tissue was significantly increased,expressions of pro-inflammatory cytokines IL-1β,IL-6,and TNF-α were decreased,while anti-inflammatory cytokines IL-4,IL-10 and TGF-β were significantly increased in hippocampus.The number of activated microglia/macrophage IBA-1 was significantly decreased,expression levels of iNOS and CD11b were decreased,while expression levels of CD206 and Arg1 were significantly increased.mRNA and protein levels of Notch1,p21 and Hes1 were significantly reduced(all P<0.05).Conclusion:Overexpression of miR-145 promotes M2 polarization of microglia to regulate post-traumatic neuroinflammatory response and improve behavioral dysfunction by increasing Treg level,which may be mediated by Notch signaling pathway.

Objective To explore the tranquilizing effects of Imaginary Working by investigating the timing characteristics of the alpha band electroencephalogram ( EEG ) power. Methods College students as volunteer subjects were recruited and randomly divided into experimental group and control group at 1∶1 ra-tio. The experimental group received Imaginary Working intervention while the control group did not re-ceive any intervention. After Imaginary Working intervention training, the alpha band absolute power of two groups was measured to compare and analyze. Results Alpha band EEG absolute power in the two groups were statistically different at all time periods (P<0. 05). The absolute power value of the experi-mental group was higher than the control group, appeared more steady than the control group at each time point. Conclusion Imaginary Working can induce the alpha band rhythm response and maintain the effects with time as a subsequent effect. Imaginary Working presents with an apparent tranquilizing effect.

From September to October 2018, a questionnaire survey was conducted on the status of diabetes management, basic equipment allocation, complication screening and follow-up, basic drug supply and health education among 136 grassroots institutions in Nanjing. Doctors in the 136 grassroots institutions who had received diabetes specialist training accounted for 18.2% (358/1 968) of total general practitioners. The management rate of diabetes patients was 98.67% (196 352/199 000) , and the standard management rate was 88.63%(174 024/196 352).The screening and follow-up rates of diabetic complications were 89.0%(121/136) and 61.2%(74/121) for glycosylated hemoglobin; 64.7%(88/136), 58.0%(51/88) for urine microalbumin;51.5% (70/136), 62.9%(44/70) for diabetic foot; 44.9%(61/136), 42.6%(26/61) carotid ultrasonography; 32.4%(44/136) and 59.1%(26/44) for fundus examination; 17.6%(24/136) and 33.3%(8/24) for ankle brachial index. The rates of screening for glycosylated hemoglobin, urinary microalbumin, diabetic foot, carotid B ultrasound, fundus and ankle brachial index in urban areas were significantly higher than those in the suburbs (both P<0.05). The diabetes health education was regularly conducted in 92.0%(126/136) of grassroots institutions. At present, the grassroots institutions in Nanjing have established health archives for most diabetic patients and conducted health education regularly, but the professional training and the screening of diabetic complications need to be strengthened.