1.Apoptotic Effects of 6-Gingerol in LNCaP Human Prostate Cancer Cells.
Hyun Woo KIM ; Deuk Hee OH ; Chaeyong JUNG ; Dong Deuk KWON ; Young Chai LIM
Soonchunhyang Medical Science 2011;17(2):75-79
OBJECTIVE: 6-Gingerol, one component of ginger (Zingiber officinale) compound, has been known to possess anti-inflammatory, analgesic, anti-emetic, and anti-cancer effects. In this study, the apoptotic ability of 6-gingerol was investigated in human prostate cancer cells. METHODS: 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) assay, flow cytometry, and western blot analysis were done in LNCaP human prostate cancer cell lines treated with the various doses of 6-gingerol for the different durations of drug exposure. RESULTS: 6-Gingerol in doses ranging from 100 to 300 microM induced dose- and time-dependent inhibition of cell viability in prostate cancer cells by using MTT assay. Maximal inhibition of cell viability was observed at 300 microM of 6-gingerol for 48 hours treatment in LNCaP cells. 6-Gingerol at the dose of 100 microM did not produce any significant change in apoptotic cells in flow cytometry analysis. However, significant increase in sub-G0/G1 phase was observed in cells treated with 200 and 300 microM of 6-gingerol. Any significant cell cycle arrest was not induced by 6-gingerol. In western blotting analysis, expression of caspase-3 was not evident in cells treated with 6-gingerol for 24 hours. However, 48 hours treatment with 6-gingerol altered the expression of caspase-3 in LNCaP cells. Expression of cleaved poly showed the dose-dependent fashion in both 24 hours and 48 hours treatment of 6-gingerol. CONCLUSION: These observations suggest that 6-gingerol may induce apoptosis in LNCaP human prostate cancer cells.
Apoptosis
;
Blotting, Western
;
Caspase 3
;
Catechols
;
Cell Cycle Checkpoints
;
Cell Line
;
Cell Survival
;
Fatty Alcohols
;
Flow Cytometry
;
Ginger
;
Humans
;
Poly(ADP-ribose) Polymerases
;
Prostate
;
Prostatic Neoplasms
2.Double Pylorus: A Case report.
Myeong Seong OH ; Chang Hwan LEE ; Jin Hee LEE ; Taik LEE ; Dae Ghon KIM ; Deuk Soo AHN
Korean Journal of Gastrointestinal Endoscopy 1993;13(2):367-369
A Case of double pylorus, in 56 year old man, was diagnosed by fiberoptic gastroscopy and upper gastrointestinal series. Two ovoid large openings of pyloric canal divided by smooth thickened septum were observed endoscopically And the relevant literatures on tihe subject were reviewed.
Gastroscopy
;
Humans
;
Middle Aged
;
Pylorus*
3.Right Angled Z-osteotomy in Total Cranial Vault Remodeling in Sagittal Synostosis.
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2002;29(4):348-351
Sagittal synostosis is the most common isolated craniosynostosis and usually recognized in early infancy. Numerous surgical techniques including strip craniectomy, the pi procedure, subtotal calvariectomy have been used, and they are usually successful if carried out in early infancy. Unfortunately, some children with sagittal synostosis are not considered for surgical correction by the age of 2 years. From March 2001 to February 2002, we have experienced two children older than 2 years of age with sagittal synostosis, and in all cases, performed total cranial vault remodeling with minimal bony defect. Reconstruction consisted of shortening of the sagittal midline strut, barrel stave osteotomy of temporal region, and right angled Z-osteotomy of parieto-occipital region. Using right angled Z-osteotomy, we can easily reshape and fix parieto-occipital bone flap with good stability and esthetic results. We report our technique for the late correction of scaphocephaly in two children with satisfactory results
Child
;
Craniosynostoses*
;
Humans
;
Osteotomy
4.Production of extracellular enzymes and histamine release from rat peritoneal mast cells by aeromonas hydrophila.
Seok Don PARK ; Jung Woon KO ; Byung Deuk JEON ; Seon Hee OH ; Chang Ho SONG ; Myoung Ok KOH
Korean Journal of Dermatology 1991;29(6):745-758
No abstract available.
Aeromonas hydrophila*
;
Aeromonas*
;
Animals
;
Histamine Release*
;
Histamine*
;
Mast Cells*
;
Rats*
5.Expression and characterization of a mutant recombinant blood coagulation factor VIII (rFVIII (m))..
Sang Hwan OH ; Sun Young CHO ; Hyun Sun JIN ; Deuk Lim JEONG ; Sung Gil JUNG ; Keun Hee OH
Experimental & Molecular Medicine 2002;34(3):233-238
In an earlier study, a site directed mutant rFVIII (rFVIII(m), Arg(336) -> Gln(336)) expressed in baculovirus-insect cell (Sf9) system was found to sustain high level activity during incubation at 37 for 24 h while the cofactor activity of normal plasma was declined steadily. In this study, a mutant B-domain deleted rFVIII(m), Arg(336) -> Gln(336) expressed in baculovirus-insect cell (Sf9) system was characterized for its enzymatic and chemical properties. The expressed rFVIII(m) and plasma FVIII (pFVIII) were purified by immunoaffinity column chromatography and identified by Western blot analysis. The partially purified rFVIII(m) exhibited cofactor specific activity of 2.01 X 10(3)units/mg protein. The molecular weight of rFVIII(m) ranged between 40 to 150 kDa with a major band at 150 kDa. Treatment of both rFVIII(m) and pFVIII with thrombin increased their cofactor activity in a similar pattern. Treatment of both the activated rFVIII(m) and native FVIII with APC decreased their cofactor activities, however, the former exhibited a slower decrease than the latter, although no significant difference was present. rFVIII(m) formed a complex with vWF, resulting in a stabilized form, and the lag period of thrombin-mediated activating was extended by vWF association. These results implicated that rFVIII(m) expressed in baculovirus-insect cell system had a comparable capacity as FVIII cofactor activity and might be a good candidate for the FVIII replacement therapy for hemophilia A patients.
Animals
;
Baculoviridae/genetics
;
Cell Line
;
Factor VIII/biosynthesis/*genetics/isolation & purification/*metabolism
;
Insects
;
Macromolecular Systems
;
Mutation/*genetics
;
Protein C/pharmacology
;
Recombinant Proteins/biosynthesis/*genetics/isolation & purification/*metabolism
;
Thrombin/pharmacology
;
von Willebrand Factor/metabolism
6.Breast Cancer Cutaneous Metastasis at Core Needle Biopsy Site.
Eujin CHO ; Min Ho KIM ; Sang Hee CHA ; Sang Hyun CHO ; Se Jeong OH ; Jeong Deuk LEE
Annals of Dermatology 2010;22(2):238-240
Cutaneous metastasis from breast cancer can occur by direct invasion, lymphatic and vascular spread as well as iatrogenic implantation. Metastasis that occurs by iatrogenic implantation after needle biopsy is very rare but the potential risk must be considered. In this report, we describe a case of breast cancer cutaneous metastasis that occurred by iatrogenic implantation following core needle biopsy. A 53-year-old woman presented with a 1x1 cm sized erythematous nodule at the biopsy site after breast conserving surgery for primary cancer. Histopathological findings confirmed cutaneous metastasis. The possibility of this consequence must be considered when performing needle biopsies.
Biopsy
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Biopsy, Large-Core Needle
;
Biopsy, Needle
;
Breast
;
Breast Neoplasms
;
Female
;
Humans
;
Mastectomy, Segmental
;
Middle Aged
;
Neoplasm Metastasis
7.Apoptotic Effects of 6-Gingerol in Human Breast Cancer Cells.
Hyun Woo KIM ; Deuk Hee OH ; Jeong Tae KOH ; Young Chai LIM
International Journal of Oral Biology 2015;40(4):223-228
6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.
Apoptosis
;
Breast Neoplasms*
;
Breast*
;
Caspase 3
;
Cell Line
;
Epithelial Cells
;
Flow Cytometry
;
Humans*
;
MCF-7 Cells
8.A Case of Cutaneous Cholesterol Embolism.
Seok Hun OH ; Hyun Ho PARK ; Jeong Deuk LEE ; Nack In KIM
Korean Journal of Dermatology 2003;41(8):1121-1123
Cutaneous cholesterol embolism is a disease due to the embolism of cholesterol crystals from ulcerated atheromatous plaques to peripheral vessels of the skin. It has been associated with major vessel surgery, angiography, angioplasty, intra-aortic balloon pump placement, anticoagulation, thrombolytic therapy and cardiopulmonary resuscitation. In addition, it may occur spontaneously. In paraffin-fixed sections, the cholesterol crystals are dissolved and leave needle-like clefts within the lumina of arterioles. We report a case of cutaneous cholesterol embolism in a 72-year-old woman who had purplish gangrenous lesions on both her feet after coronary angiography and percutaneous transluminal coronary angioplasty.
Aged
;
Angiography
;
Angioplasty
;
Angioplasty, Balloon, Coronary
;
Arterioles
;
Cardiopulmonary Resuscitation
;
Cholesterol*
;
Coronary Angiography
;
Embolism
;
Embolism, Cholesterol*
;
Female
;
Foot
;
Humans
;
Plaque, Atherosclerotic
;
Skin
;
Thrombolytic Therapy
;
Ulcer
9.A case of Unilateral Incomplete Quadruplicated Ureter associated with Ipsilateral Single Ectopic Ureteral Orifice and Renal Dysplasia.
Hee Jong JEUNG ; Dong Deuk KWON ; Bong Ryoul OH ; Soo Bang RYU ; Yang Il PARK
Korean Journal of Urology 1998;39(5):507-509
Among developmental abnormalities of the upper urinary tracts, duplication of the upper urinary tract is a relatively common finding, while triplication of the pelvis and ureter is a rare finding. Furthermore, quadruplication of the upper urinary tract is exceedingly unusual. We present a case of unilateral incomplete quadruplicated ureter that was associated with ipsilateral ectopic ureteral orifice and renal dysplasia.
Pelvis
;
Ureter*
;
Urinary Tract
10.Protective effect of lycopene against cytokine-induced β-cell apoptosis in INS-1 cells.
Kyong KIM ; Se Eun JANG ; Gong Deuk BAE ; Hee Sook JUN ; Yoon Sin OH
Journal of Nutrition and Health 2018;51(6):498-506
PURPOSE: Lycopene, a carotenoid with anti-oxidant properties, occurs naturally in tomatoes and pink grapefruit. Although the beneficial effects of lycopene on various disorders have been established, little attention has been paid to the possible anti-diabetic effects of lycopene focusing on β-cells. Therefore, this study investigated the potential of lycopene to protect β-cells against apoptosis induced by a cytokine mixture. METHODS: For toxicity experiments, the cells were treated with 0.1 ~ 10 nM of lycopene, and the cell viability in INS-1 cells (a rat β-cell line) was measured using a MTT assay. To induce cytokine toxicity, the cells were treated with a cytokine mixture (20 ng/mL of TNFα+20 ng/mL of IL-1β) for 24 h, and the effects of lycopene (0.1 nM) on the cytokine toxicity were measured using the MTT assay. The expression levels of the apoptotic proteins were analyzed by Western blotting, and the level of intracellular reactive oxidative stress (ROS) was monitored using a DCFDA fluorescent probe. The intracellular ATP levels were determined using a luminescence kit, and mRNA expression of the genes coding for anti-oxidative stress response and mitochondrial function were analyzed by quantitative reverse-transcriptase PCR. RESULTS: Exposure of INS-1 cells to 0.1 nM of lycopene increased the cell viability significantly, and protected the cells from cytokine-induced death. Lycopene upregulated the mRNA and protein expression of B-cell lymphoma-2 (Bcl-2) and reduced the expression of the Bcl-2 associated X (Bax) protein. Lycopene inhibited apoptotic signaling via a reduction of the ROS, and this effect correlated with the upregulation of anti-oxidative stress response genes, such as GCLC, NQO1, and HO-1. Lycopene increased the mRNA expression of mitochondrial function-related genes and increased the cellular ATP level. CONCLUSION: These results suggest that lycopene reduces the level of oxidative stress and improves the mitochondrial function, contributing to the prevention of cytokine-induced β-cell apoptosis. Therefore, lycopene could potentially serve as a preventive and therapeutic agent for the treatment of type 2 diabetes.
Adenosine Triphosphate
;
Animals
;
Apoptosis*
;
B-Lymphocytes
;
Blotting, Western
;
Cell Survival
;
Citrus paradisi
;
Clinical Coding
;
Luminescence
;
Lycopersicon esculentum
;
Oxidative Stress
;
Polymerase Chain Reaction
;
Rats
;
RNA, Messenger
;
Up-Regulation