1.Prevalence of antibodies to the phase I antigen of coxiella burnetii , the Q fever agent, among residents in Korea.
Sang Nae CHO ; Mi Kyeong LEE ; Jae Myun LEE ; Joo Deuk KIM ; Won Young LEE
Journal of the Korean Society for Microbiology 1992;27(3):283-288
No abstract available.
Antibodies*
;
Coxiella burnetii*
;
Coxiella*
;
Korea*
;
Prevalence*
;
Q Fever*
2.Immunoblotting analysis of antibodies against borrelia burgdorferi, the lyme disease agent, in sera from the Korean residents.
Sang Nae CHO ; Tae Yoon LEE ; Mi Kyeong LEE ; Dook Soon KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(3):263-272
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Immunoblotting*
;
Lyme Disease*
3.Comparison of Mycobacterial Growth in Dubos Medium, Hyaluronate Supplemented Medium and Umbilical Cord Extract Based Medium.
Young Nam LEE ; Joo Deuk KIM ; Joon LEW
Yonsei Medical Journal 1977;18(2):130-135
This is a report of attempts to compare the growth yields of various species of fastidious mycobacterium inch1ding human pathogens and non-pathogens in the conventional Dubos liquid medium and two simple media formulated recently; one is a medium containing 0.1% hyaluronic acid and 6.0% bovine serum albumin and the other is a semisyntheic medium made of umbilical cord extract supplemented with 10% sheep serum as a final concentration. All mycobacterial strains employed in experiments gave the heaviest growth yields in the hyaluronic acid-bovine serum albumin medium (HAS medium), among the three media. Dubos liquid medium seemed to be inferior to a medium made of umbilical cord extract (UCE medium) in supporting mycobacterial growth. There were three-to seven-fold increases in dry weight of the bacteria grown in the HAS medium as compared with those in the Dubos liquid medium. We also looked for the possible effect of bovine serum albumin (BSA)in the HAS medium on mycobacterial growth. As a result, we found that the amount of BSA in the HAS medium, ranging from zero to 6.0% in the medium, showed no substantial effect on the mycobacteria1 growth.
Comparative Study
;
Culture Media/standards*
;
Female
;
Human
;
Hyaluronic Acid/isolation & purification
;
Hyaluronic Acid/pharmacology*
;
Mycobacterium/growth & development*
;
Pregnancy
;
Tissue Extracts*
;
Umbilical Cord*
4.Expression of the 38 kDa Protein of Mycobacterium tuberculosis in M . bovis BCG and Use in the Serodiagnosis of Tuberculosis.
Sang Nae CHO ; Hee Jin KIM ; Hye Young LEE ; Seung Chul KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1999;34(6):555-559
The 38 kDa protein of Mycobacterium tuberculosis, which was known previously as antigen 5, has been extensively used in the serodiagnosis of tuberculosis. In an attempt to develop and evaluate a serodiagnostic test using the antigen, we expressed the 38 kDa protein in BCG and its seroreactivity was compared to that expressed in Escherichia coli. The coding region of the 38 kDa protein was amplified by PCR, and the gene was cloned into a Mycobacterium-E. coli shuttle expression vector pYMC-his and pQE30 expression vector and expressed in BCG and E. coli, respectively. Both recombinant 38 kDa proteins showed strong seroreactivity against pooled serum from tuberculosis patients. There was no significant difference in seroreactivity between the two recombinant antigens in sera from the far advanced tuberculosis patients. However, of 25 tuberculosis patients graded as ""minimal"" by chest X-ray, 5 (20.0%) were seropositive by r38 kDa expressed in E. coli, while 8 (32.0%) by that expressed in BCG. Likewise, higher seroreactivity by r38 kDa expressed in BCG was found in sera from the moderately advanced tuberculosis. This study thus indicates that the recombinant 38 kDa expressed in BCG is more effective than that expressed in E. coli in detecting antibodies to the native 38 kDa protein of M. tuberculosis in sera from minimally affected tuberculosis patients.
Antibodies
;
Clinical Coding
;
Clone Cells
;
Escherichia coli
;
Humans
;
Mycobacterium bovis*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymerase Chain Reaction
;
Serologic Tests*
;
Thorax
;
Tuberculosis*
5.The Normal Disk and its Anomalies.
Seong Deuk KIM ; Ouk CHOI ; Joo Hwa LEE
Journal of the Korean Ophthalmological Society 1976;17(2):177-180
The examination of the optic nerve is an important part of every examination of the fundus. In attempting to determine weather or not a pathologic process is present, it is essential that the normal disk and its varitions, as well as the congenital anomalies, be thoroughly understood. For this reason the major portion of this study will be devoted to the normal disk shape and its congenital variations.
Optic Nerve
;
Weather
6.Prevalence of antibodies to the coxiella burnetii phase II antigen among residents in korea.
Sang Nae CHO ; Sun Hee BAEK ; Yun Sop CHONG ; Joo Deuk KIM ; Won Young LEE
Journal of the Korean Society for Microbiology 1993;28(3):223-228
No abstract available.
Antibodies*
;
Coxiella burnetii*
;
Coxiella*
;
Korea*
;
Prevalence*
7.Construction of a Mycobacterium - Escherichia coli Shuttle Vector and Use in the Expression of Foreign Genes in Mycobacteria.
Sang Nae CHO ; Joo Deuk KIM ; Hye Young LEE ; Hee Jin KIM
Journal of the Korean Society for Microbiology 1997;32(3):293-300
The ability to introduce recombinant DNA molecules back into mycobacteria would greatly increase the potential of molecular genetic approaches for the study of mycobacteria as well as for the use in clinical purposes. We have initiated the construction of vectors that facilitates the introduction of recombinant DNA into mycobacteria. The vector was designed to contain replicons for multiplication in mycobacteria and Escherichia coli, a promoter for gene expression, a drug resistant gene for selecting transformants, and a few restriction enzyme sites for convenient cloning. Constructed Mycobacterium-E. coli shuttle vector named p YMC (hsp60) was shown to transform M. smegmatis at high efficiency and maintain plasmid at stable level. The ability of the vector to express cloned foreign gene was also monitored by measuring the expressed level of luciferase gene which was used as a reporter. High level of luciferase activity in M. smegmatis with pYMC (hsp60:luc) was detected confirming successful construction of Mycobacterium-E. coli shuttle vector.
Clone Cells
;
Cloning, Organism
;
DNA, Recombinant
;
Escherichia coli*
;
Escherichia*
;
Gene Expression
;
Genetic Vectors*
;
Luciferases
;
Molecular Biology
;
Mycobacterium*
;
Plasmids
;
Replicon
8.Comparison of enzyme-linked immunosorbent assay, indirect immunofluorescent antibody test and indirect immunoperoxidase antibody test in setecting antibodies to rickettsia tsutsugamushi.
Jeon Soo SHIN ; Sang Nae CHO ; Millina LEE ; Yunsop CHONG ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(1):79-85
No abstract available.
Antibodies*
;
Enzyme-Linked Immunosorbent Assay*
;
Orientia tsutsugamushi*
;
Rickettsia*
9.Detection of Mycobacterium leprae in Skin Biopsy Sepcimens From Leprosy patients by Polymerase Chain Reaction.
Kyeong Han YOON ; Sang Nae CHO ; Jung Bok LEE ; Joo Deuk KIM
Korean Journal of Dermatology 1994;32(3):409-415
BACKGROUND: Polymerase chain reaction(PCR) has brought an oppotunity for rapid detection of Mycobacterium leprae in clinical pecimens for the diagnosis of leprosy. Th DNA segment specific to M. leprae was detectable in a matteir of hours and DNA from one orgnisa appeared positive by PCR. However, the PCR tool has not been evaluated using elinical specimeriis from leprosy patients and controls. OBJECTIVE & METHODS: The primers amplifying 372bp segment of rebetitive sequence of M. leprae DNA were used in PCR. Skin biopsy specimens from 102 leprosy patient, and controls were examined for the presence of M. leprae by PCR and the results were aomared with microscopic and histopathologic findings. RESULTS: 1. As a result, of PCR after DNA preparation of M. leprae, six other mycobacteria, ten other bacteria, and skin from leprosy with five other skin biopsy tissues, 372bp DNA fragment was specifically amplified from M. leprae. 2. Dot blot, hybridization of PCR products showed that the 372bp DNA from skin biopsy specimens were derived from M. leprae. 3. As a result of PCR after DNA preparation of 10-fold diluted M. legrae from mouse footpad, PCR gave a positive result as low as one organism. 4. Of 87 specimens in which acid-fast bacilli were found under microcopic examinations 97% had positive PCR results. 5. Of 97 specimens which hadihistopathologic evidences of leprosy 95% had positive PCR results. 6. Of 15 specimens in which acid-fast bacilli were not found under n!icroscopic examinations 73% had positive PCR results. In three of five cases which had neither histopathologic nor microscopic evidences of leprosy had positive PCR results. CONCLUSION: PCR method amplifying 372bp fragment of repetitive seqi,ence was highly sensitive and specific in detecting M. leprae DNA in skin biopsy specimens, thus may be a useful tool as an additive diagnostic method, espcially for cases where microscopic antihystopathologic findings are not definite.
Animals
;
Bacteria
;
Biopsy*
;
Diagnosis
;
DNA
;
Humans
;
Leprosy*
;
Mice
;
Mycobacterium leprae*
;
Mycobacterium*
;
Polymerase Chain Reaction*
;
Skin*
10.A Case of Rickettsia Typhi.
Deuk Hwan JUN ; Sang Joo HAN ; Bae Young KIM ; Won Il PARK ; Kyung Ja LEE
Journal of the Korean Pediatric Society 1989;32(12):1736-1739
No abstract available.
Rickettsia typhi*
;
Rickettsia*