Objective Chitosan has been widely studied in the fields of biomedical materials and tissue engineering,due to its good water solubility,biocompatibility,biodegradability,film-forming property,hemostasis,wound healing and anti-inflammatory effects.Flies maggot shell contains much chitosan,which is one of the excellent raw materials.The aim of this study is to provide integral biological assessment on chitosan trauma dressing.Methods Chitosan samples were subjected to physicochemical analysis (relative molecular mass,degree of deacetylation,and content of heavy metal),bacteriostatic test,skin sensitization,cytotoxicity tests as well as hemolysis test according to national standard of China.Results The relative molecular mass of chitosan was 266 ku,the degree of deacetylation of chitosan was 75％,and the content of heavy metal was less than 10-5.The cytotoxicity level was evalnated at the first level which indicating the low cell toxicity.Skin sensitization test showed that no sensitization reaction was practically detected.The hemolysis rate was 0.769％,which was less than 5％,indicating zero hemolysis.Conclusions Chitosan has good character,which may meet the requirements of biological and medical application.