?-elemene that is an element of the Essential oil of the Cuicu-ma Wenyujin showed appreciable anticancer ability. The experimental results showed that the RNA polymerase activity was inhibited by ?-elemene at concentrations of 0.0074 mol/L~0.0172 mol/L. The study of thermal denaturation indicated that the Tm of DNA was decreased after adding ?-elemene. The fluorescent analysis of ?-elemene bound to DNA showed that the intensity of fluorescence was increased. The peak of absorption of DNA was shifted from 260 nm to 240 nm after treatment with ?-elemene.

Forty-two hyperthyroid patients with mild hepatic dysfunction were treated with 131I once orally and followed up for 6 months. The changes of hepatic functions (ALT, AST, ALP, A/G) were determined before and after treatment, hepatic function was ameliorated in these patients. The results indicate that 131I-iodine is safe and effective in treating hyperthyroidism with mild hepatic dysfunction.

Fibroblast growth factor(FGF)-21 is a novel insulin-independent glucose regulator,and can be a potential therapeutics for treatment of type Ⅱ diabetes.Although FGF-21 was discovered recently,the insight into its biology and therapeutic utility is rapidly evolving.A number of key metabolically-linked molecules and pathways have been suggested to be involved in the mechanism of action of FGF-21,which enables us to renew the understanding of the FGF-21.The aim of this review is to report the update research outcomes.

Objective To investigate the nerve recanalization and the motor function of hind legs after transplantation of BDNF genetically modified neural stem cells(NSCs) at spinal cord injury site in rat. Methods After L4 spinal cord transection of rat, BDNF genetically modified NSCs were transplanted immediately. Retrograde HRP tracing through sciatic nerve were practiced at 1 week, 1 month, 2 month, 3 month after transplantation of BDNF genetically modified NSCs. The morphological changes were observed at section of spinal cord and the motor functions of both hind legs of rat were detected. Results The morphology of the injured spinal cord sections turned better. Retrograde HRP tracing through sciatic nerve showed some HRP positive neurons and nerve fibers at the site of near rostral end of the nearly injured part at one month after transplantation and increased with the time going by. Motor function of hind legs of rats recovered significantly in all transplantation groups. Conclusion BDNF genetically modified NSCs have repairing effect on spinal cord injury in rat.

Objective To observe the changes of the gene expressions at spinal cord injury site of rat after transplantation of BDNF genetically modified neural stem cells(NSCs) so as to provide basic data for the repair of spinal cord injury. Methods The Wistar rats were randomly divided into 4 groups: control group, operation group, NSCs transplantation group, BDNF NSCs transplantation group. Four time points(7 day, 1 month, 2 month, 3 month) were divided for each group. The expressions of ? galactosidase and BDNF, GFAP, NF 200 at the site of spinal cord injury were observed by cell transplantation, X gal histochemistry, immunocytochemistry, in situ hybridization, etc. Results After transplantation of BDNF genetically modified NSCs, some X gal positive cells were found at the sections of spinal cord injury. The expressions of BDNF were strong, especially at 1 week and 1 month post transplantation in transplantation group. The GFAP and NF 200 positive cells were also found at each time point in each group. Conclusion BDNF genetically modified NSCs can survive at the site of spinal cord injury and can strongly express BDNF, suggesting that BDNF genetically modified NSCs can be used as the material for the repair of spinal cord injury.

Objective To clone the full length staphylococcal protein A(SPA) gene from Staphylococcus aureus (ATCC6538), and subsequently study the gene structure and antibody binding ability.Methods The full length and the functional region of the SPA gene were cloned into pHisSUMO vector respectively, and expressed in E. coli. The full length and the functional fragment of the SPA protein were detected for antibody binding ability and stability. The functional fragment of the SPA protein fused with SUMO was coupled to the CNBr-activated agarose for antibody purification from rabbit serum. Results A variant of the full length SPA gene was cloned, which has been submitted to GenBank (the accession number is EU695225). Two fusion proteins had the same antibody binding ability as the untagged SPA protein. However, the formers was more stable than the latter at the tested conditions. SUMO-SPA conjugated-agarose kept high efficiency for antibody binding. Conclusion To our knowledge, the full length SPA gene of S.aureus(ATCC6538) is a novel variant. The SUMO tag can improve the stability of the functional region of the SPA protein without damaging the antibody binding ability. This fusion protein has been used for antibody purification successfully.

In this study gloxylic acid-induced fluorescent histochemical method was usedto reveal the innervation of adrenergic nerves,acetycholinesterase (AChE) methodto show the cholinergic nerves,and the immunohistochemical method to display thevasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) immunoreactivenerves.This observation on the vas deferens showed that the VIP-immunoreactivenerves had the same distribution as the cholinergic nerves,and mainly existed inlamina propria,while NPY-immunoreactive nerves had the same distribution asadrenergic nerves and mainly existed in the muscular layer.One group of animalswas treated by intraperitoneal injections of 6-OHDA on the lst,2nd,6th and 7thday,and killed on the 8th day.Following treatment with 6-OHDA,significantevanescence of most adrenergic nerves was observed.NPY-immunoreactive nervesreduced remarkably and the extent of the decrease was similiar to that of adrenergicnerves,whereas no change was observed on the VIP and cholinergic nerves.Theimmunoelectronic microscopy (PAP pre-embedding method) demonstrated that VIPimmunoreactive complex deposited in the varicosity containing small clear vesicles(diameter of 40-55nm).Some varicosities also contained small number of largegranular vesicles with the diameter of 100-144mm.NPY immunoreactive complexdeposited in the large granular vesicles,and occasionally can be observed in thesmall granular vesicles,40-55nm in diameter.The results from this study suggestthat VIP probably co-exist with ACh and NPY with noradrenalin in rat vasdeferens.

By using the histochemical method, the electron microscopy and biochemical measurement, this study deals with the changes of the innervation of the uterus in tats induced by pregnancy. The results revealed that the adrenergic and cholinergic innervation of the uterus were reduced in advanced pregnancy. The content of noradrenaline(NE), expressed as ng/per gram wet weight was 194.79?2.85 in the control group and 78.56?0.48 in the late pregnancy group (P