1.Relationship between serum specific IgE and allergen skin test in allergic patients of Wuhan area.
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(2):223-4
In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70% (P < 0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.
Allergens
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China
;
Dermatophagoides farinae/immunology
;
Immunoglobulin E/*blood
;
Rhinitis, Allergic, Perennial/*immunology
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Skin Tests
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Urticaria/*immunology
2.Endotoxin and House Dust Mite Allergen Levels on Synthetic and Buckwheat Pillows.
Hae Seon NAM ; Choon Sik PARK ; Julian CRANE ; Rob SIEBERS
Journal of Korean Medical Science 2004;19(4):505-508
Pillows are known to contain significant levels of indoor allergens and endotoxin, that are of importance to house dust mite sensitized asthmatics. Buckwheat pillows are commonly used in Korea. We studied the levels of the house dust mite allergen, Der f 1, and endotoxin on new synthetic and buckwheat pillows and their accumulation over three months. Endotoxin levels were significantly higher on new buckwheat pillows compared to synthetic pillows; geometric mean levels (95% CI) were 60,950 EU/g (30,270-122,700) and 4,887 EU/g (2,570-9,311) respectively (p<0.001). No Der f 1 was detected on the new pillows. After three months Der f 1 levels were similar on buckwheat and synthetic pillows, geometric mean levels (95% CI) were 1.16 microgram/g (0.02-8.13) and 1.08 microgram/g (0.19-1.68) respectively. These results indicate that buckwheat pillows are a source of very high endotoxin levels that may be of relevance to asthma severity of atopic asthmatics.
Allergens/*immunology
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Animals
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Antigens, Dermatophagoides/*immunology
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Asthma/immunology
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*Bedding and Linens
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*Buckwheat
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Dermatophagoides farinae/immunology
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Endotoxins/*immunology
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Humans
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Korea
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Research Support, Non-U.S. Gov't
3.An analysis of skin prick test reactivity to dust mite in overweight and normal weight children with allergic asthma before and after specific immunotherapy.
Jian WANG ; Ying HUANG ; Xue-Li ZHANG ; Xia HUANG ; Xiao-Wen XU ; Fan-Mei LIANG
Chinese Journal of Contemporary Pediatrics 2016;18(4):329-334
OBJECTIVETo study the skin prick test (SPT) reactivity to house dust mite allergens in overweight and normal weight children with allergic asthma before and after standard subcutaneous specific immunotherapy.
METHODSTwo hundred and fifteen children with allergic asthma who had positive SPT responses to Dermatophagoides pteronyssinus (DP) and Dermatophagoides farinae (DF) were enrolled. According to the weight index, they were classified into overweight (n=63) and normal weight groups (n=152). Skin indices (SI) to DP and DF were compared between the two groups at 6 months and 1 year after standard subcutaneous specific immunotherapy.
RESULTSThe overweight group had a significantly larger histamine wheal diameter than the normal weight group after controlling the variation in testing time (P<0.05). After controlling the variation in weights, there were significant differences in the SIs to DP and DF before specific immunotherapy and at 6 months and 1 year after specific immunotherapy. At 6 months and 1 year after specific immunotherapy, the SIs to DP and DF were significantly reduced in both groups (P<0.05), and the overweight group had greater decreases in the SIs to DP and DF than the normal weight group.
CONCLUSIONSThe overweight children with allergic asthma have stronger responses to histamine than the normal weight patients. Specific immunotherapy can reduce the reactivity to dust mite allergens in children with allergic asthma. Within one year after specific immunotherapy, the overweight children with allergic asthma have a significantly greater decrease in the reactivity to dust mite allergens than the normal weight patients.
Adolescent ; Animals ; Asthma ; immunology ; therapy ; Child ; Child, Preschool ; Dermatophagoides farinae ; immunology ; Dermatophagoides pteronyssinus ; immunology ; Female ; Humans ; Immunotherapy ; Male ; Overweight ; immunology ; Pyroglyphidae ; immunology ; Skin Tests
4.Results of skin prick test in 908 children with asthma.
Yue WANG ; Xuan ZHANG ; Chao WANG ; Meng-Rong LI ; Chang-Chong LI ; Ying-Chun LI
Chinese Journal of Contemporary Pediatrics 2009;11(7):559-561
OBJECTIVETo report the results of skin prick test in 908 children with asthma in order to provide references for treatment of asthma.
METHODSSkin prick test was performed using ALK-Abell's inhaled prick reagents and the German Merck company's food prick reagents. Histamine was used for positive control, and normal saline, for negative control.
RESULTSSkin prick test showed positive in 703 cases (77.4%). The positive rates of inhaled and food allergens were 76.9% and 37.1%, respectively. Dermatophagoides culinae and house dust mite were two common inhaled allergens (72.4% and 74.7% respectively). Shrimp was the most common food allergen (22.9%), followed by tuna (7.3%) and mussels (6.7%). The strongest response of skin prick test was usually caused by dermatophagoides culinae (64.0%) and house dust mite (66.4%), followed by mould 1 (7.1%). The positive rate of inhaled and food allergens increased with increasing age (p<0.05).
CONCLUSIONSThe positive rate of skin prick test in the 908 children with asthma was higher. These results of this study may be useful in an epidemiological survey and specific immunotherapy of asthma.
Adolescent ; Age Factors ; Allergens ; immunology ; Animals ; Asthma ; immunology ; therapy ; Child ; Child, Preschool ; Dermatophagoides farinae ; immunology ; Humans ; Infant ; Skin Tests
5.The effect research of specific immunotherapy of allergic rhinitis and allergic rhinitis combined bronchial asthma.
Bangyu DENG ; Yunxia ZHAO ; Yiwen YOU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(12):1090-1093
OBJECTIVE:
To study the therapeutic effects of the specific immunotherapy (SIT) on allergic rhinitis and allergic rhinitis combined bronchial asthma. METHED: All patients were classified into allergic rhinitis group (AR group) with 32 patients and allergic rhinitis combined bronchial asthma group (AR+BA group) with 32 patients. Another health control group with 32 cases was designed as well. The allergens,symptom scores and therapeutic effects of the former two-group patients were analysis, and the serums of all three-group cases were extracted to evaluate the specific Immunoglobin E(sIgE), Interleukin-4 (IL-4). The SPSS13. 0 package was applied to conduct t-test and chi-square test, and the difference of P<0. 05 was regarded as statistical significance.
RESULT:
The main allergens of 64 patients were dermatophagoides farinae and dermatophagoides pteronyssinus. The improvement of symptom scores before and after SIT was statistical significant with P<0. 05. Although total effective rate reached 100% , AR group was superior than AR+BA group in term of the efficacy comparison, and P<0. 05 indicated the statistical significance. The serum sIgE, IL-4 values of three groups were brought into comparison, and P<0. 05 indicated the statistical significance of the difference.
CONCLUSION
The SIT on the AR, AR+BA is a safe and effective treatment, but different disease responds diversely. The long-term treatment course is recommended.
Allergens
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immunology
;
Animals
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Asthma
;
therapy
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Dermatophagoides farinae
;
immunology
;
Dermatophagoides pteronyssinus
;
immunology
;
Humans
;
Immunoglobulin E
;
blood
;
Immunotherapy
;
Interleukin-4
;
blood
;
Rhinitis, Allergic
;
therapy
;
Treatment Outcome
6.Detection of serum specific IgE in 437 children with allergic disease.
Ying-Hong ZENG ; Dan ZHANG ; Ye SHU ; Lei SUN ; Zhu WEI ; Bin ZHOU ; Jian-Ping TANG
Chinese Journal of Contemporary Pediatrics 2009;11(7):543-545
OBJECTIVETo investigate the distribution of allergens in children with allergic disease in Changsha.
METHODSTotal IgE and specific IgE against some allergens were examined by the AllergyScreen system in the sera of 437 children from Changsha with allergic disease.
RESULTSThe positive rates of total IgE and special IgE were 68.9% and 69.1% respectively. Dermatophagoides farina, beef, mutton, milk and fur of cats and dogs were found as common allergens. The IgE positive rate against dermatophagoides farina was the highest in children with allergic rhinitis (86.0%), followed by in those with atopic dermatitis (41.2%), eczema (27.6%) and urticaria (20.0%). Serum IgE was positive against the fur of cats and dogs in 23.5% of children with atopic dermatitis and in 18.1% of children with eczema. The IgE positive rate was lower in children with urticaria and allergic rhinitis (10.0% and 8.7% respectively). IgE positive rates against milk, beef and mutton were high in the above four allergic diseases (range: 14.0%-24.0%), but no significant differences were observed for the IgE positive rate among the four allergic diseases. The IgE positive rate to inhalant allergens in children at ages of less than 3 years was markedly higher than that in children at ages of more than 3 years (p<0.01).
CONCLUSIONSAllergic diseases are mainly mediated by allergens. Allergens, especially dermatophagoides farina, fur of cats and dogs, beef, mutton and milk, play an important role in childhood allergic diseases in Changsha.
Adolescent ; Age Factors ; Animals ; Child ; Child, Preschool ; Dermatophagoides farinae ; immunology ; Female ; Humans ; Hypersensitivity ; etiology ; immunology ; Immunoglobulin E ; blood ; Infant ; Male
7.Clinical efficacy and safety of sublingual immunotherapy using standardized Dermatophagoides farinae extract for children with combined allergic rhinitis and asthma syndrome.
Chi-jun WEN ; Ming-feng ZHU ; Wen-mei REN ; Xiao-ying LIU ; Hong QIAN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(5):393-396
OBJECTIVETo study the clinical efficacy of sublingual immunotherapy using standardized Dermatophagoides farinae extract for children with combined allergic rhinitis and asthma syndrome.
METHODSFifty-two children, from 4 to 14 years of age, with mite-sensitive combined allergic rhinitis and asthma syndrome were treated sublingually with standardized Dermatophagoides farinae extract. The clinical efficacy was evaluated by monthly follow-up visits. After treatment for 1 or 2 years using the standardized Dermatophagoides farinae extract, the asthma and rhinitis symptom scores, medication scores and adverse reactions before and after treatment were evaluated. SPSS 17.0 software was used to analyze the data.
RESULTSThe allergic asthma symptom scores before treatment during the day were 3.22 ± 0.66 and at night 2.05 ± 0.57. After 1 year of treatment, the day and night scores (1.68 ± 0.61, 0.94 ± 0.32) respectively, were decreased significantly (q values were 15.25 and 13.78 respectively, all P < 0.01). After 2 years of treatment, the scores (0.61 ± 0.28, 0.43 ± 0.13) were also decreased significantly (q values were 10.29 and 6.07 respectively, all P < 0.01). The allergic rhinitis symptom scores and medication scores were 2.34 ± 0.59 and 3.09 ± 1.01 respectively before treatment and 1.21 ± 0.46 and 1.89 ± 0.64 after 1 year of treatment. The differences were significant (q values were 15.48 and 18.61 respectively, all P < 0.01). The allergic rhinitis symptom scores and medication scores were 1.02 ± 0.37 and 1.49 ± 0.38 after 2 years of treatment. There was no significant difference between 2 years of treatment and 1 year of treatment (q values were 2.53 and 2.78 respectively, all P > 0.05). There were no severe adverse events during the treatment, except for mild mouth cavity discomfort.
CONCLUSIONSSublingual immunotherapy using standardized Dermatophagoides farinae extract is safe and effective in the treatment of children with combined allergic rhinitis and asthma syndrome.
Administration, Sublingual ; Adolescent ; Animals ; Antigens, Dermatophagoides ; administration & dosage ; immunology ; Asthma ; therapy ; Child ; Child, Preschool ; Dermatophagoides farinae ; immunology ; Female ; Humans ; Immunotherapy ; Male ; Rhinitis, Allergic, Perennial ; therapy ; Treatment Outcome
8.Efficacy and safety of cluster immunotherapy for 154 patients with allergic rhinitis.
Luo ZHANG ; Cheng-Shuo WANG ; Xiang-Dong WANG ; Yan ZHAO ; Jian-Hui LIU ; Wei ZHANG ; Bing ZHOU ; De-Min HAN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(3):187-191
OBJECTIVETo evaluate the efficacy and safety of cluster immunotherapy with Dermatophagoides pteronyssinus for allergic rhinitis.
METHODSOne hundred and fifty-four patients with allergic rhinitis to Dermatophagoides pteronyssinus were allocated to receive specific immunotherapy in a 6-week cluster schedule during the incremental-dose phase. Thereafter, these patients received maintenance-dose injection at 6-week intervals until the end of 1 year of treatment. Symptom scores and medication scores were used to evaluate the clinical efficacy and adverse reactions were recorded. A rhinoconjunctivitis quality of life questionnaire (RQLQ) was completed in the baseline and after one year treatment.
RESULTSCluster immunotherapy significantly reduced the symptom scores and total medication score of patients enrolled (P < 0.01). The immunotherapy group also had a significant improvement in the Rhinoconjunctivitis Quality of Life Questionnaire. During the one-year of treatment, there were 26 systemic adverse reactions (0.75% of all injection) in 9 patients (5.9%) and no fatal systemic reactions occurred.
CONCLUSIONSThe cluster immunotherapy is efficacious and safe to treat allergic rhinitis.
Adolescent ; Adult ; Allergens ; Animals ; Antigens, Dermatophagoides ; immunology ; Child ; Dermatophagoides farinae ; immunology ; Female ; Humans ; Immunotherapy ; adverse effects ; Male ; Middle Aged ; Rhinitis ; parasitology ; therapy ; Treatment Outcome ; Young Adult
9.Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis.
Joon Soo PARK ; Hae Seon NAM ; Yong Bae KIM ; Young Jin CHOI ; Sang Han LEE ; Sung Ho KIM
The Korean Journal of Parasitology 2007;45(3):239-243
Many allergists are currently focusing on the development of new diagnostic tools, and are attempting to improve both the sensitivity and specificity. A multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is one of the most popular diagnostic tools used in the Republic of Korea. However, there remains controversy among allergists with regard to the cut-off point for a positive result. The present study was conducted in order to determine the validity of MAST-CLA as compared with that of the skin prick test, with particular emphasis on arthropod allergens, on the basis of percentage agreement rates and k-values, and also to suggest the optimal positive cutoff points using receiver operating characteristic (ROC) curves. The study was conducted with 97 subjects (54 men, 43 women). Optimal individual cut-off points were calculated as follows; class II for Dermatophagoides farinae, class I for Dermatophagoides pteronyssinus, and trace for a cockroach mix. These findings suggest that attempting to apply optimal individual cut-off points will be a good way of improving diagnostic tests, particularly MAST-CLA.
Adult
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Allergens/*immunology
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Animals
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Antigens, Dermatophagoides/*immunology
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Chemiluminescent Measurements/*methods/standards
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Cockroaches/chemistry
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Dermatophagoides farinae/chemistry
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Dermatophagoides pteronyssinus/chemistry
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Female
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Humans
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Hypersensitivity/*diagnosis/immunology
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Insect Proteins/*immunology
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Male
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*ROC Curve
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Skin Tests/methods
10.Study on the efficacy and safety of sublingual immunotherapy with standardized dermatophagoides farinae drops for allergic rhinitis.
Shaopeng HUANG ; Xiufang XIE ; Yong CHEN ; Lihua WU ; Ruiyu LI ; Fan SHEN ; Hong CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(7):618-621
OBJECTIVE:
To evaluate the efficacy and safety of the sublingual immunotherapy with dermatophagoides farinae drops on patients with allergic rhinitis.
METHOD:
One hundred and twelve cases were collected from adult patients with dust-mite allergic rhinitis of our hospital who could adhere to treatment and regular follow-up. These patients were randomly allocated to receive either sublingual immunotherapy (SLIT group, n = 56) or medical treatment (Control group, n = 56). To evaluate the clinical efficacy by side effects which were registered, symptom and medication scores which were assessed and rhinoconjunctivitis quality of life questionnaire (RQLQ) which was completed in the baseline and two years after treatment.
RESULT:
Dropouts after the 2 years' treatment were 5 of SLIT group and 4 of Control group respectively. SLIT group induced the significant reductions on both the symptom scores (7.81 ± 3.14 to 3.89 ± 2.01, P < 0.0 1) and the medication scores (2.86 ± 0.75 to 0.44 ± 0.06, P < 0.01). Meanwhile, Control group induced the reductions on both the symptom scores (8.01 ± 3.32 to 5.20 ± 2.43) and the medication scores (2.95 ± 0.80 to 1.75 ± 0.40). There were significant differences (P< 0. 01) in symptom and medication scores between the two groups after 2-year treatment. The patients in SLIT group had fewer symptoms and lower intake of medication. There were statistically significant differences in RQLQ between SLIT group [19 (15,22)] and Control group [36 (26,47)] after two years treatment (Z = -5. 21, P < 0.01). SLIT group also had significant improvement in RQLQ (Z = -6.10, P < 0.01) between before and after the treatment. There were 4 patients who showed adverse reactions in SLIT group (3 occurred in increment period, and 1 occurred in the maintenance period). The incidence of adverse reactions was 7.14%. No severe systemic side effects were registered.
CONCLUSION
SLIT with standardized dermatophagoides farinae drops in China is safe and effective to patients with allergic rhinitis.
Administration, Sublingual
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Adult
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Animals
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Antigens, Dermatophagoides
;
immunology
;
China
;
Dermatophagoides farinae
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Humans
;
Quality of Life
;
Rhinitis, Allergic
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drug therapy
;
Sublingual Immunotherapy
;
Treatment Outcome