Objective To find a sensitive index which can reveal the mild cognitive impairment in non-demented patients with Parkinson’s disease (PD).Methods Thirty PD patients without clinical dementia and 34 age-matched normal controls performed a matching-to-sample task while event-related potential (ERP) was recorded from their scalp. There were two kinds of stimulus pairs in this study: condition 1, the second stimulus (S2) in a pair was identical to the first one (S1) (match condition); condition 2, S2 conflicted with S1 in their color attribute (conflict condition). Subjects were required to press a button in the match condition and another button in the conflict condition.Results In the match condition, P100, N150, P180, N200, and P300 were elicited by S2. In the conflict condition, potentials following N200 went continuously to negativity and formed another component,N270,before P300. The N270 component was evoked by S2 of conflict condition in both controls and patients. The patient group showed a delayed and smaller N270 than the control group[peak latency: P3,patients:(317?37) ms,controls:(298?31) ms;P4, patients: (313?35) ms,controls: (293?34) ms;amplitude: P3,patients: (-3.06?2.51) ?V,controls: (-4.55?2.86) ?V]. The peak amplitude of P300 was decreased in the patient group [P4,patients: (6.94?3.95) ?V,controls: (9.58?3.80) ?V], but the peak latency of P300 did not differ significantly between the two subject groups. There was no difference between the two groups in peak latency and amplitude of N150.Conclusion PD patients have cognitive impairment even in the absence of a clinical dementia. N270 is subtler than P300 in revealing the mild cognitive impairment.

Objective To assess the value of muhichannel epidural somatosensory evoked potential (SEP) in cortical mapping of primary sensorimotor areas for electrode implantation of motor cortex electrical stimulation.Methods Intraoperative multichannel epidural SEP was recorded in 13 patients with intractable neuropathic pain.SEP mapping was used for determining the location of sensorimotor areas.Results The typical phase reversals of N20 and P20 were recorded and the location of the central sulcus and the primary sensorimotor area were accurately determined in 11 patients.The location of the primary sensorimotor failed to be determined in the other 2 patients because of the atypical SEP.Conclusion The intraoperative muhichannel epidural SEP is reliable in the cortical mapping.

By tracing of ~(75)Se-Na_2SeO_3 incubating and irrigating of the isolated myocardial slices of rats, the numeric relationship between the free selenics in the serum and the serum sdenics, the sodium sdenite doses injected were observed; the absorption of the frce selenics and the serum selenics was compared; the setenics metabolic rate and quantities of the myocardial tissue in different duration after injecting of selcnite, the effect of selenics concentration to the participating were calculated, It is suggested that there is a very close relationship between the free selenics and the selenium metabolism in the myocardium; the ratio betwen the values of the free selenics and the serum selenics may become a stable indicator of the selenium metabolism; the free selenics in the serum may be where the important mediates of selenium metabolism are.

se—Met. In the experimental range of concentration, the activities of GSH—px were higher than that of control.

The cytogenotoxic effect of Na_2SeO_3 on lymphocytes in human whole blood culture was studied. This was done by means of analysis of sisterchromatid exchange (SCE) and determination of unscheduled DNA synthesis (UDS) with the use of scintillometric procedure and autoradiographic observation. The experiments showed that Na_2SeO_3induced a significant increase of SCE value and higher levels of UDS. These results indicate that Na_2SeO_3 has DNA-damaging potential and cytogenotocity, its threshold concentration was 6.48?10~(-7)mol/L.

Objective To evaluate the surgical method and results of the treatment of chronic rupture of the Achilles tendon using V Y tendinous flap. Methods Nine cases with old rupture of the Achilles tendon, with a mean course of illness of 112 days, were treated with V Y tendinous flap and the tendon gap was 3.2～ 6cm,averaging 4.6cm. Results The mean period of follow up was 2.7 years. The Arner Lindholm method was used to assess the results of surgical treatment, 7 cases of the 9 had excellent results (77.8% ) and 2 cases had good results (22.8% ), with the excellent and good rate being 100% . Conclusion The V Y tendinous flap is a simple and good method for treatment of old rupture of the Achilles tendon.

Objective:To examine influences of attention on the conflict monitoring system. Methods:Thirty normal adults participated in the matching-to-sample task. They were divided into two subgroups. One subgroup was required to attend to the color while ignoring the value attribute of a number pair. The other subgroup was required to attend to the value while ignoring the color attribute of a number pair. Subjects were asked to press one of the two buttons according to whether the two digits were identical in the attended attribute and event-related potentials were recorded on their scalps. Results: A N270 component of event-related potential was recorded to the conflicting stimulus pairs but not to the matching pairs. The amplitude of N270 increased and its duration prolonged under attended condition. However, its onset latency showed no significant changes. Conclusion: The conflict monitoring process is automatically initiated and then regulated and enhanced by the attention control system.

Objective To investigate the relationship between EphA7 protein and carcinogenesis and development of pancreatic cancer by detecting the expression of EphA7 protein in pancreatic cancer tissues. Methods The expression of EphA7 in 10 cases of normal pancreatic tissue, 51 cases of pancreatic cancer and its adjacent tissues were detected with immunohistechemieal methods and the relationship between EphA7 and pathologic features of pancreatic cancer were analyzed. Results The rate of expression of EphA7 protein in normal pancreatic tissue was 10% (1/10), 47.1% (24/51) in adjacent pancreatic cancer tissues, 94.1% (48/51) in pancreatic cancer tissues. There were significant difference among the three groups (P <0.05). There was no significant correlation between the expression of EphA7 protein and the age, sex, tumor location, tumor size in patients with pancreatic cancer (P > 0.05). However there was significant correlation between the expression of EphA7 protein and the degree of differentiation, clinical staging, lymph node metastasis, or distant metastasis (P < 0.05). Conclusions The abnormally high expression of EphA7 may be relevant with the occurrence and development of the pancreatic cancer.

Objective To study the inhibitory mechanism of soliquiritigenin on tyrosinase.Methods The inhibition effect and inhibitory kinetics of tyrosinase induced by oliquiritigenin were investigated.The effect of isoliquiritigenin on A375 melanoma cell were preliminarily indicated.Results The inhibitory effect of isoliquiritigenin on monophenolase and diphenolase of tyrosinase was good, IC50 was (11.22 ±0.92) μM and (48.53 ±4.75) μM, isoliquiritigenin was a competitive inhibitor, the value of inhibition constant ( Ki ) was ( 12.14 ±0.54 ) μM.Isoliquiritigenin could inhibit the prolifevation of A375 cell significantly, and the tyrosinase activity and melanin synthesis decreased.Conclusion Isoliquiritigenin as a tyrosinase inhibitor, plays an important role in the regulation of melanin, which provides the theoretical basis for the clinical anti skin cancer.

Objective To study the incidence and clinical significance of abnormal neuroendocrine hormone in patients with the syndrome of brain injury.Methods 67 cases with the syndrome of brain injury were included in the study group,and 95 cases without the post traumatic syndrome after brain injury were included in the control group.The level of FT3,FT4,TSH,growth hormone(GH),andrenocortico hormone(ACTH),cortisol (Cor),prolactin(PRL),testosterone (T),estradiol (E2),follicular stimulating hormone (FSH),luteotropic hormone (LH),and progesterone (P) in peripheral blood were measured by radioimmunoassay.The incidence of the abnormal neuroendocrine hormone after brain injury was statistically analyzed.Patients with abnormal hormone were given hormone replacement therapy and the curative effects were observed.Results The incidence of neuroendocrine hormone abnormalities was 38.8％ in patients with the syndrome of brain injury,while it was 10.5％ in the control group.There was significant difference between the 2 groups(P＜0.05).The symptom remission rate was 88.5％ after hormone treatment.Conclusions There was a high incidence of abnormal neuroendocrine hormone secretion in patients with the syndrome of brain injury.The hormone level may be used as an important index to guide clinical therapy.