1.Construction, expression and enzymatic activity analysis of AUR1 eukaryotic expression vector of Botrytis cinerea.
Yongchun QIU ; Xiaoping LIU ; Ping GOU
Chinese Journal of Biotechnology 2013;29(1):78-86
In order to study the expression and the activity of inositol phosphorylceramide synthase (BcAUR1 gene) in Botrytis cinerea, we amplified BcAUR1 by RT-PCR from Botrytis cinerea, using the special primers with FLAG and BamH I/Xho I restriction sites. Recombinant pYES2-BcAUR1 was constructed to transform into Saccharomyces cerevisae deltayorl by LiAC. The expression of inositol phosphorylceramide (IPC) synthase and its activity were detected by Western blotting and HPLC, respectively. The results show that pYES2-BcAUR1 could express in uracil mutant deltayorl of Saccharomyces cerevisae. IPC synthase enzyme activity of pYES2-BcAUR1 transformants significantly increased and was approximately double than no-load BcAUR1 transformants. The low concentration of Aureobasidin A could inhibit growth of no-load BcAUR1 transformants, but pYES2-BcAUR1 transformants could resist fungal growth inhibition which was induced by Aureobasidin A.
Botrytis
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enzymology
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genetics
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Depsipeptides
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pharmacology
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Fungal Proteins
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genetics
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metabolism
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Gene Expression
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Genetic Vectors
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Hexosyltransferases
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genetics
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metabolism
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Saccharomyces cerevisiae
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genetics
;
metabolism
2.Romidepsin (FK228) improves the survival of allogeneic skin grafts through downregulating the production of donor-specific antibody via suppressing the IRE1α-XBP1 pathway.
Yuliang GUO ; Siyu SONG ; Xiaoxiao DU ; Li TIAN ; Man ZHANG ; Hongmin ZHOU ; Zhonghua Klaus CHEN ; Sheng CHANG
Journal of Zhejiang University. Science. B 2022;23(5):392-406
Antibody-mediated rejection (AMR) is one of the major causes of graft loss after transplantation. Recently, the regulation of B cell differentiation and the prevention of donor-specific antibody (DSA) production have gained increased attention in transplant research. Herein, we established a secondary allogeneic in vivo skin transplant model to study the effects of romidepsin (FK228) on DSA. The survival of grafted skins was monitored daily. The serum levels of DSA and the number of relevant immunocytes in the recipient spleens were evaluated by flow cytometry. Then, we isolated and purified B cells from B6 mouse spleens in vitro by magnetic bead sorting. The B cells were cultured with interleukin-4 (IL-4) and anti-clusters of differentiation 40 (CD40) antibody with or without FK228 treatment. The immunoglobulin G1 (IgG1) and IgM levels in the supernatant were evaluated by enzyme-linked immunosorbent assay (ELISA). Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and western blotting were conducted to determine the corresponding levels of messenger RNA (mRNA) and protein expression in cultured cells and the recipient spleens. The results showed that FK228 significantly improved the survival of allogeneic skin grafts. Moreover, FK228 inhibited DSA production in the serum along with the suppression of histone deacetylase 1 (HADC1) and HDAC2 and the upregulation of the acetylation of histones H2A and H3. It also inhibited the differentiation of B cells to plasma cells, decreased the transcription of positive regulatory domain-containing 1 (Prdm1) and X-box-binding protein 1 (Xbp1), and decreased the expression of phosphorylated inositol-requiring enzyme 1 α (p-IRE1α), XBP1, and B lymphocyte-induced maturation protein-1 (Blimp-1). In conclusion, FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway. Thus, FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR.
Animals
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Depsipeptides
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Endoribonucleases
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Hematopoietic Stem Cell Transplantation
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Histone Deacetylase Inhibitors/pharmacology*
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Mice
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Protein Serine-Threonine Kinases
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Skin Transplantation
3.A Case of Emetic Toxin Producing Bacillus cereusStrains Isolated from Outbreak.
Jong Hyun KIM ; Eun Gyoung LIM ; Hyun Chul JANG ; Ju Young PARK ; Sun Jin LEE ; Mi Sun PARK ; Gil Bae CHOI ; Bok Kwon LEE
Korean Journal of Clinical Microbiology 2009;12(1):48-52
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction (PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
Bacillus
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Bacillus cereus
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Coloring Agents
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Depsipeptides
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Diarrhea
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Eating
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Electrophoresis, Gel, Pulsed-Field
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Enterotoxins
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Enzyme-Linked Immunosorbent Assay
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Humans
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Polymerase Chain Reaction
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Republic of Korea
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Vacuoles
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Vomiting
4.Differential proteomics on synthetic antimicrobial decapeptide against Streptococcus mutans.
Yi LIU ; Wei FEI ; Yanjun WANG ; Yandong MU ; Hongkun WU
West China Journal of Stomatology 2015;33(2):187-191
OBJECTIVETo compare the protein profiles between decapeptide-treated and untreated planktonic cells of Streptococcus mutans (S. mutans) by differential proteomic analysis to determine and identify the key proteins.
METHODSIn our previous study, we investigated decapeptide (KKVVFKVKFK-NH2), which was a novel adenosine monophosphate. Compared with other oral pathogens tested, decapeptide had a preferential antibacterial activity against S. mutans. It also inhibited S. mutans biofilm formation and reduced the one-day developed biofilm. In the present study, we first synthesized decapeptide, and then compared the protein profiles between decapeptide-treated and untreated planktonic cells of S. mutans by two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We also verified different expressions of key protein enolase in the protein level.
RESULTSThe results showed that decapeptide altered the protein expression of planktonic S. mutans. These proteins were functionally involved in carbohydrate degradation by glycolysis, protein folding, conjunction, transport, translation, adenosine triphosphate binding, protein binding, sequence-specific DNA binding, transcription factor activity, and two-component response regulator activity. Western blot results showed that enolase protein expression decreased obviously in decapeptide-treated cells of S. mutans.
CONCLUSIONThe protein expression of S. mutans significantly changed after synthetic antimicrobial decapeptide treatment, suggesting that decapeptide may present a preferential effect on oral caries by changing the expression of certain key proteins, such as enolase protein.
Anti-Bacterial Agents ; Anti-Infective Agents ; Biofilms ; Dental Caries ; Depsipeptides ; genetics ; Electrophoresis, Gel, Two-Dimensional ; Oligopeptides ; genetics ; Proteomics ; Streptococcus mutans ; metabolism
5.Romipeptides A and B, two new romidepsin derivatives isolated from Chromobacterium violaceum No.968 and their antitumor activities in vitro.
Lei XIONG ; Chang-Fa CHEN ; Tao-Ling MIN ; Hai-Feng HU
Chinese Journal of Natural Medicines (English Ed.) 2019;17(2):155-160
Romipeptides A and B (1 and 2), two new romidepsin derivatives, and three known compounds, chromopeptide A (3), romidepsin (4) and valine-leucine dipeptide (5) were isolated from the fermentation broth of Chromobacterium violaceum No. 968. Their structures were elucidated by interpretation of their UV, HR-ESI-MS and NMR spectra. The absolute configuration of compound 1 and 2 were established by single crystal X-ray diffraction analysis. Compounds 1-5 were evaluated for their anti-proliferative activities against three human cancer cell lines, SW620, HL60, and A549. The results showed most of these compounds exhibited antitumor activities in vitro, in which compound 2 displayed potent cytotoxicity to SW620, HL60 and A549 cell lines, with IC of 12.5, 6.7 and 5.7 nmol·L, respectively.
Antineoplastic Agents
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chemistry
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pharmacology
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Cell Survival
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drug effects
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Chemistry Techniques, Analytical
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Chromobacterium
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metabolism
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Depsipeptides
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chemistry
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pharmacology
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Dipeptides
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chemistry
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Drug Screening Assays, Antitumor
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Fermentation
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Humans
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Molecular Structure
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Peptides, Cyclic
;
chemistry
6.Effect of fluorouracil combined with FK228 on the proliferation, apoptosis and Fas mRNA level in HepG2 hepatoma cell lines.
Journal of Central South University(Medical Sciences) 2009;34(2):124-129
OBJECTIVE:
To explore the effect of fluorouracil (FU) combined with epigenetic drug FK228 (depsipeptide FR901228) on the proliferation, apoptosis and Fas mRNA level in HepG2 hepatoma cell lines.
METHODS:
There were 4 groups in this experiment: an untreated group, an FK228 treated group, a FU treated group,and a FU combined with FK228 group.Tetrazolium salt colorimetry (MTT) assay was used to assess the cell inhibition rate. Q value of Kingos formula and multi-factor analysis of variance (ANOVA ) were used to judge the combination treatment effect,and flow cytometry was used to detect the apoptosis rate. Fas mRNA level was analyzed by RT-PCR.
RESULTS:
FK228 or FU would inhibit the growth of HepG2 cells in a concentration-dependent and time-dependent manner. Cell inhibition rates of HepG2 were significantly enhanced in the FU combined with FK228 group, compared with that in the FU treated group alone (P<0.05). Both Q values were more than 1, the 2 drug combinations showed interaction,and FU combined with FK228 had synergistic effect.Compared with the FK228 treated group and the FU treated group, apoptosis rate of HepG2 cells was significantly increased (P<0.05), and the Fas mRNA level was up-regulated in HepG2 cells in FU combined FK228 group (P<0.05).
CONCLUSION
Combination of FK228 and FU can enhance the proliferation inhibition and apoptosis induction of FU in hepatoma cell lines, up-regulate the Fas mRNA level, and increase the sensitivity of hepatoma cell lines to FU.
Antibiotics, Antineoplastic
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pharmacology
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Antimetabolites, Antineoplastic
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pharmacology
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Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Depsipeptides
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pharmacology
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Drug Synergism
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Fluorouracil
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pharmacology
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Hep G2 Cells
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Humans
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RNA, Messenger
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genetics
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metabolism
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fas Receptor
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genetics
;
metabolism
7.Progress in cancer treatment with histone deacetylase inhibitor.
Mei DONG ; Xing-sheng HU ; Shan-shan CHEN ; Pu-yuan XING ; Feng-yi FENG ; Yuan-kai SHI
Chinese Journal of Oncology 2013;35(7):481-485
Aminopyridines
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therapeutic use
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Antineoplastic Agents
;
therapeutic use
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Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
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Benzamides
;
therapeutic use
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Clinical Trials as Topic
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Depsipeptides
;
therapeutic use
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Histone Deacetylase Inhibitors
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therapeutic use
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Histone Deacetylases
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metabolism
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Humans
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Hydroxamic Acids
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therapeutic use
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Indoles
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therapeutic use
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Neoplasms
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drug therapy
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enzymology
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Sulfonamides
;
therapeutic use
8.Progress in the study of some important natural bioactive cyclopeptides.
Wen-Yan XU ; Si-Meng ZHAO ; Guang-Zhi ZENG ; Wen-Jun HE ; Hui-Min XU ; Ning-Hu TAN
Acta Pharmaceutica Sinica 2012;47(3):271-279
Natural cyclopeptides are hot spots in chemical and pharmaceutical fields because of the wide spreading bio-resources, complex molecular structures and various bioactivities. Bio-producers of cyclopeptides distribute over almost every kingdom from bacteria to plants and animals. Many cyclopeptides contain non-coded amino acids and non-pepditic bonds. Most exciting characteristic of cyclopeptides is a range of interesting bioactivities such as antibiotics gramicidin-S (2), vancomycin (3) and daptomycin (4), immunosuppressive cyclosporin-A (1) and astin-C (8), and anti-tumor aplidine (5), RA-V (6) and RA-VII (7). Compounds 1-4 are being used in clinics; compounds 5-8 are in the stages of clinical trial or as a candidate for drug research. In this review, the progress in chemical and bioactive studies on these important natural bioactive cyclopeptides 1-8 are introduced, mainly including discovery, bioactivity, mechanism, QSAR and synthesis.
Animals
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Anti-Bacterial Agents
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chemical synthesis
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chemistry
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pharmacology
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Antineoplastic Agents
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chemical synthesis
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chemistry
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therapeutic use
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Cyclosporine
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chemistry
;
pharmacology
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Daptomycin
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chemical synthesis
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chemistry
;
pharmacology
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Depsipeptides
;
chemical synthesis
;
chemistry
;
therapeutic use
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Gramicidin
;
chemical synthesis
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chemistry
;
pharmacology
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Humans
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Immunosuppression
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Immunosuppressive Agents
;
chemical synthesis
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chemistry
;
pharmacology
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Molecular Structure
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Neoplasms
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drug therapy
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Peptides, Cyclic
;
chemical synthesis
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chemistry
;
pharmacology
;
therapeutic use
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Quantitative Structure-Activity Relationship
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Vancomycin
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chemical synthesis
;
chemistry
;
pharmacology
9.Advance of several types of important marine antitumor drugs.
Acta Pharmaceutica Sinica 2008;43(5):435-442
Marine antitumor drugs have been the research focus in the world. Recently, advancement has been made in the investigation of six types of compounds including bryostatin-1, ecteinascidin-743, dolastatin, didemnin B, psammaplin and halichondrin B. In this review, we summarized the recent research progress of the above mentioned marine antitumor drugs and their derivatives. Also, the development tendency of marine antitumor drugs was discussed.
Animals
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Antineoplastic Agents
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pharmacology
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therapeutic use
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Apoptosis
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drug effects
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Biological Products
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isolation & purification
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pharmacology
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therapeutic use
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Bryostatins
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isolation & purification
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pharmacology
;
therapeutic use
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Cell Line, Tumor
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drug effects
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Depsipeptides
;
isolation & purification
;
pharmacology
;
therapeutic use
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Dioxoles
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isolation & purification
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pharmacology
;
therapeutic use
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Disulfides
;
isolation & purification
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pharmacology
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Ethers, Cyclic
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isolation & purification
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pharmacology
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Humans
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Macrolides
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Marine Biology
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Neoplasms
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drug therapy
;
pathology
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Tetrahydroisoquinolines
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isolation & purification
;
pharmacology
;
therapeutic use
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Tyrosine
;
analogs & derivatives
;
isolation & purification
;
pharmacology
10.Inhibitory effect of a novel histone deacetylases inhibitor FK228 on human colon cancer HCT-116 cells in vitro and in vivo.
Dong-bo XU ; Yun-ling WANG ; Yuan YUE ; Shuang-chan WU ; Hong DING
Chinese Journal of Oncology 2013;35(11):814-818
OBJECTIVETo investigate the inhibitory effects of a novel histone deacetylases inhibitor FK228 on human colon cancer HCT-116 cells in vitro and in vivo, and evaluate its toxicity and side effects.
METHODSThe in vitro growth inhibitions of HCT-116 cells by different concentrations of FK228 and 5-Fu for 24, 48 and 72 h were assessed by CCK-8 assay. BALB/c nude mouse models of tumor xenografts were prepared by subcutaneous implantation of tumor tissue, and 4 mg/kg FK228 and 50 mg/kg 5-Fu were i.p. injected, respectively. The inhibitory effects on tumor growth, hematology, and liver and kidney function were evaluated.
RESULTSCCK-8 assay indicated that FK228 had an obvious growth inhibitory effect on HCT-116 cells in a dose- and time-dependent manner. The IC50 of FK228 in HCT-116 cells was 12.05 ng/ml for 48 h, while the IC50 of 5-Fu was 18.92 µg/ml. At 20 days after FK228 and 5-Fu treatment, the tumor volume of the FK228 group was (139.71 ± 44.54)mm(3), significantly lower than that of the 5-Fu group [(282.28 ± 58.81)mm(3)] and that of the model group [(520.65 ± 39.73)mm(3), P < 0.01 for both]. The average tumor weight was (0.07 ± 0.02)g in the FK228 group, significantly lower than that of the 5-Fu group [(0.20 ± 0.08)g, P < 0.01]. The tumor growth inhibition rate of the FK228 group was 73.2%, significantly higher than that of the 5-Fu group (45.8%, P < 0.01). The ALT levels of the FK228 and 5-Fu groups were significantly higher than that of the model group (P < 0.01). The BUN of 5-Fu group was significantly higher than that of the model group (P < 0.01), but the BUN of FK228 group was not significantly different from that of the blank and control groups (P > 0.05 for both). Routine blood test showed that WBC, RBC, Hb and PLT of the 5-Fu group were significantly lower than those of the model group (P < 0.05 for all), but only WBC of the FK228 group was significantly lower than that of the model group (P < 0.05). The pathological examination using HE staining revealed that in the FK228 group, there were fibrosis and inflammatory cell infiltration in the liver tissue, and mild edema of the renal tubules in the kidney. However, in the 5-Fu group there were extensive hepatocyte edema and necrosis in the liver, and evident deformation and necrosis of glomeruli and tubules, and tubular wall thinning in the kidney.
CONCLUSIONSThe results of this study indicate that FK228 can more effectively than 5-Fu inhibit the growth of HCT-116 cells in vitro and vivo, and without obvious toxic effect on the kidney and hematology. Its clinical value in colon cancer treatment deserves further investigation.
Alanine Transaminase ; blood ; Animals ; Antibiotics, Antineoplastic ; administration & dosage ; adverse effects ; pharmacology ; Antimetabolites, Antineoplastic ; pharmacology ; Blood Urea Nitrogen ; Cell Proliferation ; drug effects ; Depsipeptides ; administration & dosage ; adverse effects ; pharmacology ; Dose-Response Relationship, Drug ; Fluorouracil ; pharmacology ; HCT116 Cells ; Hematologic Tests ; Histone Deacetylase Inhibitors ; administration & dosage ; adverse effects ; pharmacology ; Humans ; Inhibitory Concentration 50 ; Kidney ; pathology ; Liver ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Random Allocation ; Tumor Burden ; drug effects ; Xenograft Model Antitumor Assays