1.Water-soluble constituents from Dioscorea zingiberensis
Deping XU ; Changying HU ; Shirong TANG ; Zijie PANG
Chinese Traditional and Herbal Drugs 1994;0(01):-
Objective To study water-soluble constituents of Dioscorea zingiberensis in order to seek active components. Methods Various chromatographic techniques were used to isolate the constituents. Structures of compounds were elucidated by spectroscopic analyses of 1H-NMR, 13C-NMR, 135DEPT, HMQC, HMBC, and TOCSY. Results One new steroidal saponin was isolated and identified as 26-O-(?-D-glucopyranosyl)-(25R)-furost-5-en-3?, 26-diol-22-OMe-3-O-{?-L-rhamnopyranosyl-(1→4)-[?-D-glucopyranosl-(1→3)-?-D-glucopyranosl-(1→2)]-?-D-glucopyranoside}. Conclusion The compound is a novel compound named as zingierenin E. The other two compounds are reported for the first time from D. zingiberensis.
2.Application of the detection of CG in clinical diagnosis
Yan WANG ; Mei CAI ; Deping YANG ; Mingrong TANG
Journal of Chinese Physician 2016;(z1):256-258
Serum Cholyglycine (CG)is a main component of human bile acid,one of the conjuga-ted bile acids formed by the combination of bile acid and glycine is synthesized in the liver cells.Glycochol-ic acid (CG)as a clinical indicator for detecting hepatobiliary disorders,and the traditional index of liver
function test compared has greater advantages,its detection for intrahepatic cholestasis,liver disease and biliary system diseases diagnosis,treatment and prognosis analysis of pregnancy to provide an important ba-sis.
3.Research progress on the development of the strategies for siRNAs delivery in vivo.
Journal of Biomedical Engineering 2012;29(4):775-779
RNA interference (RNAi) is a powerful endogenous process initiated by short double stranded RNAs, which results in sequence-specific posttranscriptional gene silencing. Because any protein that causes or contributes to a disease is susceptible to RNAi, the RNAi has high potential for therapeutic treatments. In a clinical setting, however, there are many obstacles to targeted delivery of small interfering RNA (siRNA) in vivo, specificity and stability of the RNAi reagents. In this review, we focus on recent progress in the development of efficient siRNA delivery vehicles to help the application of siRNA to in vivo therapy.
Drug Carriers
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Gene Transfer Techniques
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Humans
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Molecular Targeted Therapy
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methods
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trends
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RNA Interference
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RNA, Small Interfering
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administration & dosage
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genetics
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metabolism
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Transfection
4.Accuracy assessment of transthoracic echocardiography in diagnosing left cardiac valve calcification in elderly patients
Kang LI ; Chongqing YANG ; Ruisheng ZHANG ; Linfeng ZHANG ; Zheng WANG ; Xin QI ; Guodong TANG ; Deping LIU
Chinese Journal of Geriatrics 2017;36(4):395-399
Objective To investigate the accuracy of transthoracic echocardiography (TTE) for diagnosing left cardiac valve calcification in elderly patients.Methods In this retrospective study,410 elderly patients died in Beijing Hospital from 1982 to 2015 were collected.They all had full pathological cardiac valve examination data in the autopsy examination and full mortem TTE data.They were classified into three groups according to the year of death:group 1982-1995,group 1996-2005 and group 2006-2015,as well as into three groups according to the age of death:group 60-75,group76-90 and group 91-106.Results The sensitivity,specificity,diagnostic coincidence rate,positive likelihood ratio (PLR),negative likelihood ratio (NLR),positive predictive value (PPV) and negative predictive value(NPV) of TTE versus autopsy pathological findings as golden standard for diagnosing left cardiac valve calcification were 88.6%,28.2%,43.7%,1.234,0.405,0.298 and 0.878.Compared with group 1982-1995,the group 1996-2005 and group 2006-2015 showed the sensitivity and PPV of TTE versus golden standard autopsy findings for diagnosing left cardiac valve calcification in the elderly were increased while the specificity,PLR,NLR and NPV were decreased.Compared with group 60-75,the group 76-90 and group 91-106 showed that the sensitivity and PPV of TTE versus golden standard autopsy findings for diagnosing left cardiac valve calcification were increased,while the specificity and diagnostic coincidence rate were decreased.The sensitivity,specificity,diagnostic coincidence rate,PLR,NLR,PPV,NPV of TTE versus golden standard autopsy findings for the diagnosis of aortic valve calcification and mitral valve calcification in the elderly were 88.2% and 44.0%,30.8% and 75.3%,42.7% and 71.5%,1.275 and 1.780,0.382 and 0.744,0.250 and 0.198,0.909 and 0.906,respectively.Conclusions TTE provides high sensitivity for diagnosing left cardiac valve calcification in the elderly,especially for diagnosing the aortic valve calcification,but the specificity is not satisfactory.
5. Comparison of the efficacy of goals-activity-motor enrichment therapy and neurodevelopment therapy in early intervention of high-risk infants with cerebral palsy
De WU ; Zhenzhen CUI ; Jing ZHU ; Deping WU ; Li YANG ; Fuli LYU ; Enyao LI ; Jiulai TANG
Chinese Journal of Applied Clinical Pediatrics 2019;34(11):832-836
Objective:
To compare the efficacy of goal-activity-motor environment (GAME) therapy and neurodevelopmental therapy (NDT) in the early intervention of high-risk infants with cerebral palsy (IHRCP), and to provide scientific evidence-based medical basis for early intervention of IHRCP.
Methods:
A total of 62 cases of IHRCP were enrolled in the Children′s Neurological Rehabilitation Center of the First Affiliated Hospital of Anhui Medi-cal University from June 2017 to December 2018.They were divided into GAME group (32 cases) and NDT group (30 cases) according to the admission order.Gross Motor Function Scale (GMFM), Fine Motor Function Measure (FMFM) and Gesell Development Scale (GDS) were used for detection and comparison.The differences among the gross motor, the fine motor score and the developmental quotient (DQ) between two groups before treatment, 9 months after treatment and 12 months after treatment, and the normalization rate and the incidence of cerebral palsy between the two groups at 12 months of age were compared.
Results:
(1) Motor function was as follows: at 9 months[GAME: (32.63±15.83) scores, (30.03±15.88) scores], [NDT: (33.37±15.61) scores, (29.67±12.54) scores] and at 12 months[GAME: (40.56±15.79) scores, (36.31±14.98) scores], [NDT: (40.47±15.50) scores, (36.73±14.58) scores] after treatment, and GMFM and FMFM scores in GAME and NDT groups were significantly higher than those before treatment[GAME: (27.56±14.24) scores, (21.75±11.35) scores], [NDT: (26.93±14.96) scores, (21.30±10.67) scores], and the differences were significant (all