Aims: Dental caries is a chronic infectious disease caused by Streptococcus mutans due to its ability to form biofilm. This study aims to assess the antimicrobial efficacy of Melastoma malabathricum leaf extract against S. mutans on the surface of tooth samples as a potential therapy for dental caries. Methodology and results: Extraction of M. malabathricum leaves was done using acetone as the solvent and antibacterial activity of the extracts was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antibiofilm activity of M. malabathricum extract against S. mutans was determined by comparing the colony count, biofilm formation assay and morphology observation by scanning electron microscope (SEM). The MIC value of extracts was 6.25 mg/mL and MBC value was >25 mg/mL. A decrease in colony count was noted when tooth samples were incubated with M. malabathricum extract for 8 h compared to 4 h incubation. At pH 5, the formation of the colony was the least, medium at pH 8 and maximum at pH 7. A decrease in biofilm formation was observed when tooth samples were incubated with the extract for 8 h. SEM observations showed treatment with the extract caused S. mutans cell membrane to leak leading to cell morphology changes. Conclusion, significance and impact of study Acetone extract of M. malabathricum leaves showed excellent antibacterial activity against S. mutans. It has bactericidal activity with the ability to inhibit biofilm in dose-dependent manner against S. mutans. The morphological analyses suggested that the extract disrupted the cell membrane of the bacteria.
Dental Caries--therapy

To explore the effects of plasma jet (PJ) and plasma activated water (PAW) on the sterilization of Streptococcus mutans ( S. mutans) and compare the advantages and disadvantages of the two methods, so as to provide a basis for plasma treatment of dental caries and to enrich the treatment means of dental caries, an atmospheric pressure plasma excitation system was built, and the effects of PJ and PAW on the sterilization rate of S. mutans and the changes of temperature and pH during treatment were studied under different excitation voltage ( U _e ) and different excitation time ( t _e ). The results showed that in the PJ treatment, the difference in the survival rate of S. mutans between the treatment group and the control group was statistically significant ( P = 0.007, d=2.66) when U _e = 7 kV and t _e = 60 s, and complete sterilization was achieved at U _e = 8 kV and t _e = 120 s in the PJ treatment. In contrast, in the PAW treatment, the difference in the survival rate of S. mutans between the treatment group and the control group was statistically significant ( P = 0.029, d = 1.71) when U _e = 7 kV and t _e = 30 s, and complete sterilization was achieved with PAW treatment when U _e = 9 kV and t _e = 60 s. Results of the monitoring of temperature and pH showed that the maximum temperature rise during PJ and PAW treatment did not exceed 4.3 °C, while the pH value after PAW treatment would drop to a minimum of 3.02. In summary, the optimal sterilization parameters for PJ were U _e =8 kV and 90 s < t _e ≤ 120 s, while the optimal sterilization parameters for PAW were U _e = 9 kV and 30 s< t _e ≤ 60 s. Both treatment methods achieved non-thermal sterilization of S. mutans, where PJ required only a smaller U _e to achieve complete sterilization, while at pH < 4.7, PAW only required a shorter t _e to achieve complete sterilization, but its acidic environment could cause some chemical damage to the teeth. This study can provide some reference value for plasma treatment of dental caries.
Humans ; Streptococcus mutans ; Dental Caries/therapy* ; Sterilization ; Temperature ; Water

ABSTRACT The first dental examination is a major step that is affected by many factors and determines the dental treatment process. The main objective of this study was to evaluate the association of child temperament with child dental anxiety, parental dental anxiety, dental behaviour and dental caries. The study consisted of 100 children aged 3 to 6 who were attending their first dental examination and their accompanying parents. The Facial Image Scale (FIS), Frankl’s Behaviour Scale (FBS), Corah’s Dental Anxiety Scale (CDAS) and the Short Temperament Scale for Children (STSC) were used for assessment. The DMFT (decayed, missing and filled teeth) score of each child was recorded. Children who exhibited negative behaviour on the FBS had the highest rhythmicity scores (p = 0.008). The mean DMFT score of children in the rhythmicity temperamental dimension was relatively high (p = 0.008). The parents of children defined as negative on the FBS had high dental anxiety levels on CDAS (p < 0.001). Children whose parents had higher dental anxiety levels showed higher dental anxiety (p = 0.007). The success rate of dental treatment procedures may be increased by improving dentists’ knowledge of dental anxiety related to the child’s temperament and integrating parental support to reduce dental anxiety.
Dental Care for Children ; Dental Anxiety ; Cognitive Behavioral Therapy ; Temperament ; Dental Caries

Fused teeth were a phenomena of teeth anomalies in shape, which can affect the dental teeth both in primary and permanent dentition. Fused teeth do not only cause problems on crowding of dentition, abnormal occlusion and aesthetic, but also increase risks of dental caries, endodontics diseases, periapical diseases and periodontal diseases. Fusion of deciduous teeth may lead to abnormality of subsequent permanent teeth. Treatment of fused teeth may require multidisciplinary approach in endodontics, periodontics, oral and maxillofacial surgery, prosthodontics and orthodontics. The aim of the present article is to review the etiology, classification, clinical manifestations and treatment of fused teeth in order to provide dental clinicians with a reference of clinical management for fused teeth.
Humans ; Fused Teeth/therapy* ; Anodontia ; Tooth, Deciduous ; Dental Caries/therapy* ; Esthetics, Dental

OBJECTIVETo evaluate the difference between chemomechanical and traditional mechanical caries removal in indirect pulp capping of the symmetric primary molars.

METHODSA total of 192 children with deep caries in the two symmetric primary molars were studied. The two symmetric primary molars were divided into two groups for treatment with chemomechanical method with Carisolv(TM) system or traditional mechanical method. After caries removal, calcium hydroxide agent was placed as the protective base, and glass ionomer cement was used to restore the teeth. The patients were followed up every 1 month within one year after the operation.

RESULTSThe percentage of healthy primary molar pulp was 95.3% with chemomechanical method and 87.0% with traditional method, showing a significant difference between the two methods.

CONCLUSIONChemomechanical caries removal is more effective in preserving the healthy pulp than the traditional method.

Child ; Dental Caries ; therapy ; Dental Pulp Capping ; methods ; Follow-Up Studies ; Humans ; Molar ; Tooth, Deciduous

Deciduous caries and abnormal tooth development are common children's oral diseases which seriously harm the health of deciduous dentition, hinder the absorption of nutrients and affect the general growth and development. Caries of deciduous teeth progresses rapidly and can develop into rampant caries and other hard tissue diseases in a short time. The metal prefabricated crown is a stainless steel restoration for the treatment and repair of severe dental tissue damage in children. Its repair technique has certain operating procedures, requires specific equipments and materials, needs qualified dental clinicians to complete. The Society of Pediatric Dentistry, Chinese Stomatological Association organized experts to formulate the guideline of the clinical operation stainless steel crown restoration, so as to standardize the application of stainless steel crowns in the molar repair treatment of deciduous teeth such as caries in children and to further promote the application of the technique.
Child ; Crowns ; Dental Caries ; therapy ; Dental Restoration, Permanent ; Humans ; Stainless Steel ; Tooth, Deciduous

Vital pulp therapy(VPT)is an important pathway to preserve and maintain pulp tissue in a healthy state. VPT has been improved recently as the new progress achieved in pathobiology, bioactive materials and clinical research. The present review summarizes the clinical outcomes and prognostic factors of VPT, including direct pulp capping, partial pulpotomy and full pulpotomy in mature permanent teeth with carious pulp exposure, and briefly introduces the new progress in this field.
Calcium Compounds ; Dental Caries/therapy* ; Dental Pulp Capping ; Dentition, Permanent ; Humans ; Pulpotomy ; Silicates ; Treatment Outcome

Crown Lengthening ; methods ; Crowns ; Dental Caries ; therapy ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Fractures ; therapy

OBJECTIVETo determine the economic effects on the prevention of dental fear (DF) by pre-operation-education or local anesthesia method during the process of tooth filling.

METHODSOne hundred and fifty school children of age 7 to 12, participating in this study, suffered from occlusal caries on their mandibular first molars. They were divided into 3 groups, with 25 boys and 25 girls each. Group A (pre-operation-education) was showed video tape on caries to, familiarized with clinic environment, including dental chairs and instruments; Group B (local anesthesia) received injection of 1.8 ml of 2% lidocaine for local anesthesia about 15 minutes before treatment; Group C (blank) received no intervention for DF. Thereafter, all subjects received same filling treatment. During that process, each DF case was evaluated blindly based on Venham's clinical ratings of anxiety and cooperation. Cost-minimization analysis was then studied.

RESULTSSignificant difference was found between groups, but not between group A and B in DF rate (5/50, 9/50 and 21/50, chi(2) = 15.503 1, P = 0.000 4). The costs of each case in 3 groups were 83.99 Yuan, 87.09 Yuan and 87.76 Yuan respectively.

CONCLUSIONBetween pre-operation-education and local anesthesia methods, the former was worth promoted for it's better clinical effect in DF prevention with lower cost.

Child ; Dental Anxiety ; economics ; epidemiology ; prevention & control ; Dental Caries ; therapy ; Dental Cavity Preparation ; economics ; methods ; Female ; Humans ; Incidence ; Male

The purpose of this study is to evaluate salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva of the head and neck cancer patients. Twenty three cancer patients (19 males, 4 females) who had undergone chemotherapy and radiation therapy and twenty four healthy volunteers (14 males, 10 females) as a control were included. Salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva were examined. Compared to saliva of the control group, salivary flow rate (p<0.001) and salivary pH (p<0.001) were significantly lower in head and neck cancer patients. The colony counts of Lactobacilli was higher in head and neck cancer patients (p<0.05) than in control group. These salivary factors and cariogenic activity can increase the prevalence of dental caries in head and neck cancer patients.
Dental Caries ; Drug Therapy ; Head and Neck Neoplasms* ; Healthy Volunteers ; Humans ; Hydrogen-Ion Concentration ; Male ; Prevalence ; Saliva*