1.Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan.
Ji Hyung KIM ; Dennis K GOMEZ ; Toshihiro NAKAI ; Se Chang PARK
Journal of Veterinary Science 2010;11(1):85-87
In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.
Animals
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DNA, Bacterial/genetics
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Disease Outbreaks/*veterinary
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Electrophoresis, Agar Gel/veterinary
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Fish Diseases/genetics/*microbiology
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Flavobacteriaceae Infections/microbiology/*veterinary
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Flavobacterium/genetics/*isolation & purification
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Genetic Variation/*genetics
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Japan
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*Osmeriformes
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Plasmids/genetics