Immunochromatography is a rapid test for the detection of anti-Dengue IgM and IgG. The result can be read within 7 minutes. This method is highly sensitive (100%) and specific (87%).
Dengue ; diagnosis

A descriptive prospective study of hospitalized Dengue including Dengue Fever (OF) and Dengue Haemorrhagic Fever (DHF) was undertaken in An Giang General Hospital. An Giang Province from April 2002 to May 2003. A first blood sample (BS) will be taken on admission to detect dengue antibody by Elisa (IgM and IgG) and virus isolation. The second BS will be drawn on discharge with the same test. If the patient is discharged before day 10th after the onset of fever, a third BS will be planned, for IgM and IgG too. In the hospital, all patients will be follow-up adequate platelet count and haematocrit. - There is 1009 patient selected (less than 15 years old), 5 DHF deaths due to DHF and 4 deaths due to others. Classification of the Dengue hospitalized cases: DF (28. 5%), DHF (49%) and DSS (10%). - The highest positive IgM rate is belong to DSS patient group (89%) and then DHF, the lowest rate is DF (34%). The virus isolated rate of the DSS patient group is highest (16.8%), and then DF (14.6%). DEN2 is predominant (with 84% of all virus isolated), and then DEN4 (8.8%). - On the admission, there are the overload for Dengue diagnosis. 12% cases with DF/DHF on the admission but they are typhoid fever, measles and viral infection on the discharge. - Clinical and laboratory findings are the more severe (thrombocytopenia and haemoconcentration), the more positive IgM rate and virus isolated. - For the correspondences between DF, DHF diagnosis. Clinical and laboratory findings according to WHO criteria: there are only 22.2% of DHF satisfied 4/4 WHO criteria, 39.2% satisfied 3/4 criteria and ultrasound is the test supported for these DF, DHF cases. - 71% confirmed DHF cases and 26% confirmed DSS cases have not thrombocytopenia and haemoconcentration. - The haemorrhagic manifestation just appear in 46.6% of DHF cases, even if DSS cases, there are only 56.2% cases have got the haemorrhagic manifestations.
Dengue Hemorrhagic Fever ; Dengue ; Diagnosis

A study on the changes of red blood cell, white blood cells, platelets and formulation of leukocyte with positive Mac- ELISA in 30 patients with dengue haemorrhagic fever and ages of 3 -15 shown that red blood cells of 84% patients were normal, leukocyte of 47% patients were normal, leukocytopenia (50%) in which neutrophilic multinuclear leukocytopenia (67%); the increased rate of lymphatic leukocyte but normal amount of these. Thrombocytopenia (severe: 20%; quite severe (50%); moderate (10%); Leuko thrombopenia (43,3%).
Dengue ; Child ; Diagnosis

There are about 20 millions of people infected by dengue virus and 500 cases of haemorrhagic fever admitted to hospital every year in the world in which the mortality rate of the disease was 5%, even 15%. In Vietnam, the dengue fever and haemorrhagic dengue fever was an epidemic that its frequency of circulation was increasingly. During recent 5 years, the morbidity number and the mortality number of dengue fever/ haemorrhagic dengue fever were 10,8431 cases and 234 deaths, respectively. The diagnosis of the disease involved the viral isolation and serum diagnosis. The treatment involved the symptomatic management, close monitoring and timely management of shock
Dengue ; diagnosis ; therapeutics

Retrospectative study on 20 infants with dengue heamorrhagic fever was confirmed by Mac ELISA at Chau Doc Hospital in 2001, study group was 6 infants < 12 months age, control group was 14 infants > 12 months age. 100% high fever; 66,7% subcutaneous peteachie; 66,7% hepatomegaly; 50% gastro-intestinal bleeding; accompany with respiratory infection were the most common signs. 100% hemoconcentration; 80% glomerule under 100.000/mm3. The rate of dengue shock syndrome was rather high. IgG antibody which infants less than 1 year old transplacetally received from their mothers caused antibody dependent enhancement of infection, so they with primary-type infection were at risk of dengue shock syndrome as in older children with secondary infection
Dengue ; diagnosis ; Fever ; Hospitals ; epidemiology

99 patients over 15 year old were treated at Hue central Hospital from July 2002 to May 2003. 45.5% was in male and 54.5% in female. The most prevalence of age group was from 15 to 24 years old (72.7%). The symptom of abdominal pain in Dengue haemorrhagic fever was 61.8% and 46.2% in Dengue fever, alvine flux in Dengue haemorrhagic fever was 58.8% and 47.7% in Dengue fever. Liver intumescence in Dengue haemorrhagic fever was 29.4% and 7.7% in Dengue fever, pain in epigastric region in Dengue haemorrhagic fever was 32.4% and 9.2% in Dengue fever. The signs of headache, retroorbital pain, muscles and joints, supracondylar intumescence and along to sternocleidomastoid muscle had equal prevalence in Dengue haemorrhagic fever and Dengue fever. Based on Dengue haemorrhagic fever degree of WHO, most of cases were in degree II (82.4%), degree I (11.8%) and degree III (5.8%). All 99 cases, who were diagnosed with Dengue haemorrhagic fever (100%) by basic systems, only 34.3% of cases with Dengue haemorrhagic fever were re-diagnosed by hospitals, the rest 65.7% cases were Dengue fever
Diagnosis ; adult ; dengue ; viruses ; epidemiology

This retrospective study was aimed to assessing clinical, paraclinical symptoms of 340 patients between 16-45 years old, with Dengue haemorrhagic fever. Patients were divided into 2 groups with and without Dengue Shock Syndrome (DSS). The results showed that clinical symptoms including sudden high fever, headache, orbital pain, congested skin, petechie, and positive tourniquet test. Paraclinical symptoms included thrombocytopenia increased haematocrite, leukopenia, increased serum transaminases. This disease often occurred in October and November. DSS can occure at 4th to 7th day with symptoms vomiting, sudden subsided fever, cold clammy skin, pleural or pericardial effusion, ascites, rapid increased haematocrite, thrombocytopenia, leukopenia. Dengue haemorrhagic fever can be cured in short time with infusion of isotonic fluid and Ringer lactate fluid
Dengue ; Hemorrhagic Disease Virus, Rabbit ; Dengue Hemorrhagic Fever ; diagnosis

BACKGROUND: Recently, dengue fever has increased throughout tropical regions and emerged as the most important vector borne viral disease in human. 4 serotypes of viruses are circulating concurrently in these regions and thus it may be anticipated to increase risk of dengue hemorrhagic fever. Even though dengue fever is still not endemic in Korea, it is necessary to test antibodies against dengue viruses because the number of Koreans who have visited these regions is continuously increasing. MATERIALS AND METHODS: Serum specimens from persons with suspected dengue fever had been collected. Commercial kit, immunochromatographic test (ICA), and the IgM capture enzyme-linked immunosorbent assay (MAC-ELISA) were employed for dengue fever detection in these studies. For confirmation randomized 25 specimens among total of 99 specimens were selected and compared with those results from commercial kit and IFA. RESULTS: 33 (33.3%) among 99 specimens showed positive antibody against dengue virus by commercial kit. Positive rate of traveller who have visited Indonesia, Philippines, Malaysia, Thiland was 54.5%. To compare the efficiency of test methods, 25 randomly selected specimens were tested by the MAC-ELISA and IFA simultaneously. 9 specimens showed postive results with the MAC-ELISA method whereas 13 speciments were positive with the IFA methods. CONCLUSION: Confirming the diagnosis of dengue fever with antibody against dengue virus was attempted for the first time in Korea. The results from our study indicate that establishing a national surveillance and/or laboratory diagnostic system in Korea are necessary. In addtion, antibody test strategies for national surveillance system should be carefully considered.
Antibodies ; Dengue Virus* ; Dengue* ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin M ; Indonesia ; Korea ; Malaysia ; Philippines ; Severe Dengue ; Virus Diseases

BACKGROUND: Recently, dengue fever has increased throughout tropical regions and emerged as the most important vector borne viral disease in human. 4 serotypes of viruses are circulating concurrently in these regions and thus it may be anticipated to increase risk of dengue hemorrhagic fever. Even though dengue fever is still not endemic in Korea, it is necessary to test antibodies against dengue viruses because the number of Koreans who have visited these regions is continuously increasing. MATERIALS AND METHODS: Serum specimens from persons with suspected dengue fever had been collected. Commercial kit, immunochromatographic test (ICA), and the IgM capture enzyme-linked immunosorbent assay (MAC-ELISA) were employed for dengue fever detection in these studies. For confirmation randomized 25 specimens among total of 99 specimens were selected and compared with those results from commercial kit and IFA. RESULTS: 33 (33.3%) among 99 specimens showed positive antibody against dengue virus by commercial kit. Positive rate of traveller who have visited Indonesia, Philippines, Malaysia, Thiland was 54.5%. To compare the efficiency of test methods, 25 randomly selected specimens were tested by the MAC-ELISA and IFA simultaneously. 9 specimens showed postive results with the MAC-ELISA method whereas 13 speciments were positive with the IFA methods. CONCLUSION: Confirming the diagnosis of dengue fever with antibody against dengue virus was attempted for the first time in Korea. The results from our study indicate that establishing a national surveillance and/or laboratory diagnostic system in Korea are necessary. In addtion, antibody test strategies for national surveillance system should be carefully considered.
Antibodies ; Dengue Virus* ; Dengue* ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin M ; Indonesia ; Korea ; Malaysia ; Philippines ; Severe Dengue ; Virus Diseases

OBJECTIVE: To investigate the rapid and accurate method for the detection of dengue virus (DENV) by using nicking enzyme assisted strand-displacement amplification (SDA) combined with gold nanoparticles-based lateral flow strip. METHODS: Total RNA of the virus was extracted by using magnetic beads method and transcribed to cDNA for SDA detection system. Nicking enzyme-assisted method was used for detecting DENV, and agarose gel electrophoresis was used for analyzing the sensitivity of SDA amplification products. A gold nanoparticles-based lateral flow strip was developed based on the principle of nucleic acid base complementary pairing to design the test line and control line. The gold particles were prepared by using sodium citrate reduction method for gold nanoparticles-based lateral flow strip construction. RESULTS: The sensitivity of the SDA method was 10 fmol/L, and the sensitivity of gold nanoparticles-based lateral flow strip based on SDA method was also 10 fmol/L. In a linear range from 10 fmol/L to 10 fmol/L, the corresponding linear correlation coefficient () of DENV was 0.98. The specificity of nanoparticles-based lateral flow strip based on SDA for DENV detection was high, which was no crossing with other control groups. CONCLUSIONS A gold nanoparticles-based lateral flow strip based on SDA method for DENV detection has been established, which is convenient, fast, and the result is visible to naked eyes.
Dengue ; diagnosis ; Dengue Virus ; isolation & purification ; Gold ; Humans ; Metal Nanoparticles ; Reproducibility of Results ; Sensitivity and Specificity