OBJECTIVETo analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells.

METHODSWe used 3'RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database.

RESULTSWe found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs.

CONCLUSIONSThere are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.

Cell Line, Tumor ; Cell Transformation, Neoplastic ; Genes, Neoplasm ; Humans ; Polyadenylation ; Stomach Neoplasms ; genetics

Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells. Methods We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90αand SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.

Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells. Methods We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90αand SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.

Objective To explore the effects of inhospital emergency process ~engineering for patients with acute poisoning.Methods Emergency Department of the Affiliated Hospital of Hangzhou Normal University implemented inhospital poisoning emergency process reengineering in March 2016.This implementation optimized original emergency process and applied it in patients with acute poisoning beginning with 6 aspects including refining precheck patients,assessment of poisoning emergency response group,fast gastrolavage,transportation,gastrolavage combined with blood purification group rapid preparation,emergency intensive care unit preparation.We compared the rescue efficiency of patients with acute poisoning before (from March 2015 to February 2016) and after (from March 2016 to February 2017) process reengineering.Results After process reengineering,the time from being admitted to hospital to beginning gastrolavage and the duration of gastrolavage was (8.91 ± 5.29)min and (31.86 ± 8.42)min respectively shorter than those before process reengineering with significant differences (t=3.397,4.028;P ＜ 0.01).After process reengineering,the time from being admitted to hospital to opening blood purification tubes (176.59 ± 88.73)min and from being admitted to hospital to starting blood perfusion (229.35 ± 108.79)min were significantly sooner than those before process reengineering (t=3.600,3.550;P ＜ 0.01).Conclusions The inhospital emergency process reengineering is scientific and convenient.It is propitious to improve rescue efficiency of patients with acute poisoning.

Objective? To explore the knowledge demand on disaster nursing of nurses so as to provide the countermeasure and suggestions for improving the knowledge on disaster nursing of nurses. Methods? From August to October 2016, a total of 510 nurses at a ClassⅢ Grade A hospital in Hangzhou City were investigated with the Disaster Nursing Training Needs Questionnaire for the nurses. The SPSS 21.0 was used to data analysis. A total of 510 questionnaires were sent out and 479 valid questionnaires were collected with 93.92% for the recovery rate. Results? A total of 479 nurses were with the high demand for the professional knowledge and management knowledge of disaster nursing along with 4 and above for the scores of all terms. In the demand of professional knowledge, the highest demand term was the self-protection knowledge (4.70±0.54). Among 9 training modes with multiple choices by 479 nurses, the highest demand for the training mode was theoretical teaching. Among different training modes, 36.7% to 54.9% of nurses selected theoretical teaching. There were statistical differences in the training contents demands among nurses with different departments (P＜ 0.05). Conclusions? Hospitals should strengthen training for the disaster nursing events capability of nurses. Different disaster nursing knowledge and skills should adopt suitable training modes. Nurses with no rescue experience need to attend more effective organizational management training in the disaster.

OBJECTIVETo investigate the effects of different concentrations of paraquat (PQ) poisoning on the expression of voltage-dependent anion channel (VDAC) and caspase family in the mitochondria of rat lung tissue, and to explore possible mechanisms of acute lung injury induced by acute PQ poisoning.

METHODSTwo hundred healthy adult Wister rats with equal numbers of male and female ones were randomly and equally divided into control group and poisoned group. The control group received one-time gastric lavage with 1 ml of normal saline, and the poisoned group with PQ (50 mg/kg) diluted in 1 ml of normal saline. Twenty rats were collected at 1, 24, 72, 120, and 168 h after lavage with normal saline or PQ and dissected after anesthesia. Mitochondria were separated from rat lung tissue, and the content of VDAC and caspase-3, -8, and -9 were determined.

RESULTSThe expression of VDAC and caspase-3, -8, and -9 in the poisoned rats were significantly higher than that in the control group (P < 0.001). At 1, 24, 72, 120, and 168 h after exposure, acute diffuse damages were found in alveolar capillary endothelial cells, alveolar epithelial cells, and pulmonary interstitial cells. Inflammatory cell infiltration in the pulmonary interstitium, alveolar structural disorder, and substantially increased fibroblasts were also found in rat lung tissue.

CONCLUSIONPQ poisoning can up-regulate the expression of VDAC and caspase-3, -8, and -9 in mitochondria of rat lung tissue to induce acute lung injury.

Acute Lung Injury ; chemically induced ; pathology ; Animals ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Caspase 9 ; metabolism ; Caspases ; metabolism ; Female ; Lung ; drug effects ; pathology ; Male ; Mitochondria ; drug effects ; metabolism ; Paraquat ; poisoning ; Rats ; Rats, Sprague-Dawley ; Voltage-Dependent Anion Channels ; metabolism