1.Platelet-rich plasma ameliorates photo-aging phenotype of fibroblasts by adjusting the generation of reactive oxygen species
Chuanlong JIA ; QingJian YANG ; Bo BI ; Tianyi LIU ; Liang CHEN ; Yu GU ; YiQun ZHOU ; Ping YANG ; NingWen ZHU ; JingJing ZHU ; Dengke QING
Chinese Journal of Medical Aesthetics and Cosmetology 2018;24(1):54-57
Objective To explore the effect of platelet-rich plasma (PRP) on the generation of reactive oxygen species (ROS) and the phenotypes of photo-aging fibroblasts.Methods A photoaging cell model by repeating UVB irradiation was treated using appropriate concentration of PRP;Cell morphology and the rate of aging dying were observed under inverted microscope 24 hours later after establishment of the cell model;The expression of ROS between experimental and control group was detected using fluorescence microscope after single UVB irradiation.The relative intensity of fluorescence was analyzed using flow cytometry.Results PRP could ameliorate the large and sprawl appearance of photoaging fibroblasts obviously,reduce the generation of ROS as well as decrease the relative intensity of ROS.Conclusions PRP can decrease the level of intracellular oxidative stress caused by UVB irradiation,reduce the generation of ROS and ameliorate the senescence-like phenotypes of pho toaging fibroblasts.
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