Adult ; Hemoperfusion ; Humans ; Male ; Paraquat ; blood ; poisoning

Objective To study the acute effects of PM2.5 on the heart rhythm of spontaneously hypertensive rats(SHR)and the mechanism.Methods Twenty-eight male spontaneously hypertensive rats(SHR)were randomly divided into four groups.PM2.5 was administered by intratracheal instillation at the doses of 0 mg/kg,7.5 mg/kg,15 mg/kg and 30 mg/kg respectively.ECGs were monitored at 30 min,1 h and 24 h later.Results The numbers of the rats with arrhythmia in all groups increased at 30 min after treatment.At 1 h after treatment,in control group the rats recovered,but in PM2.5 groups abnormal ECGs was still showed.However,ECGs of all groups became normal 24 h later.As shown by laser scanning confocal microscope(LSCM),the expression of Cx43 in the heart tissue of rats in 15 mg/kg and 30 mg/kg groups significantly decreased compared with the control group.There was no significant change in content of MDA and SOD in the heart tissue of PM2.5 treated rats.Conclusion PM2.5 exposure through inhalation may induce arrhythmia in SHR rats and the downregulated expression of Cx43 may play an important role in the pathogenesis.

Objective To study the effect of anisodamine (654-2) combined with exogenous pulmonary surfactant (PS) on tumor necrosis (TNF- ?) ,structure of lung tissue changes during pig's lung ischemic-reperfusion injury. Methods 24 pigs ,approximately 45-day-old, were randomly divided into four groups: a control group,a 654-2 group,a PS group and a 654-2 plus PS group. The TNF-? concentration of every lung was analysed with ELISA methods at the point of pre-clamping right pulmonary aortery,post-clamping 60',90'(opening),post-unclamping 60',90',120'.Finally ,we examined the pathologic changes of the lungs. Results After clamping right pulmonary artery , TNF-? concentrations in every experimental groups were significantly lower than that of the control group (all P

Objective To evaluate the application value of Microendoscopic Discectomy (MED) in the treatment of lumbar disc herniation and lumbar spinal stenosis. Methods 188 cases of lumbar disc herniation and lumbar spinal stenosis were treated by MED, 38 cases of them performed lateral recess decompression. Results The mean follow-up period was 6.9 months. excellent and good therapeutic result rate was 97.4% (Macnab's standard). Conclusions MED in the treatment of lumbar disc herniation and lumbar spinal stenosis has short-term satisfactory therapeutic efficacy.

Objective To investigate the relationship between different genotype infection and multiple infection of human papil‐lomavirus(HPV) with cervical lesions among the women in Northeast region of Sichuan province .Methods The cervical exfoliated cells in 213 women with HPV infection were performed the HPV genetype detection by the gene chip technique and the cervical le‐sion degree was also detected .Results 213 cases of cervial lesion with positive HPV infection were divided into five groups accord‐ing to pathological examination results :chronic inflammation(110 cases) ,cervical intraepithelial neoplasia(CIN) Ⅰ (21 cases) ,CINⅡ (26 cases ) ,CIN Ⅲ (28 cases ) and cervical cancer (28 cases) .The high risk HPV infection was dominated by the genotype HPV16 ,58 ,33 ,18 and the low risk HPV infection was dominated by the HPV genotype 11 ,6 .The HPV genotype HPV11/6/16 , HPV16/33 ,HPV16/6 ,HPV16/58 and HPV16/18 infection were most common in the chronic cervial inflammation ,CIN Ⅰ ,CINⅡ ,CIN Ⅲ and cervical cancer groups .The constituent ratio of different cervical lesions had statistical difference between the simple high risk HPV infection group and the low risk HPV infection group (χ2 = 41 .01 ,P< 0 .01) .The simple genotyps and the mixed genotype HPV infection had no influence on the cervical lesion degree(χ2 = 5 .74 ,P> 0 .05) .Conclusion HPV 16 ,58 ,33 ,18 are the main high risk HPV genotypes among women in the northeast region of Sichuan province ,HPV 16 is significantly related with the cervial lesion degree ;multiple HPV genotype infection does not promote the progress of cervical lesion .

Adult ; Heavy Metal Poisoning ; Hemoperfusion ; Humans ; Male ; Poisoning ; therapy ; Thallium ; poisoning

Objective To analyze the features of normal high-frequency ultrasonography of tarsal tunnel. Methods Forty volunteers (20 males and 20 females) were examined with high-frequency ultrasound (12 MHz). The tendons,nerve and blood vessels in tarsal tunnel were observed from short-axis and long-axis views dynamically. The areas of tarsal tunnel and posterior tibial nerve were measured and com-pared between the males and females. Results High-frequency ultrasonography depicted the anatomical structure of tarsal tunnel,and the tendons,nerve and blood vessels presented different sonographic features that were easy to differentiate. The area of tarsal tunnel: male (7.61 ±1.00) cm2,female (6. 61 ± 1. 07) cm2 (P <0. 01). The area of posterior tibial nerve: male (9. 59 ± 0. 75) mm2,female (8.91 ±0.74) mm2(P<0.01). Conclusion High-frequency ultrasonography can clearly show and accurately measure the tarsal tunnel structure. To be familiar with the normal ultrasonographic anatomy of tarsal tunnel will help to improve the efficacy of ultrasound diagnosis of tarsal tunnel abnormalities.

Matrix metalloproteinase-2 (MMP-2) level and the ERK1/2 signal pathway are dependent factors for the growth and metastasis of cancer. However, the impact of MMP-2 in combination with ERK1/2 in tumor patients with drug resistance is unknown. To determine the relationship between MMP-2 and the ERK1/2 signal pathway, we established an adriamycin (ADM)-induced MG-63 (ADM-MG-63) cell line. With the increase of the ERK1/2 pathway blocker PD98059, we detected the expression levels of MMP-2 and p-ERK1/2 by Western blot in ADM-MG-63 cells. In ADM-MG-63 cells transfected with MMP-2-siRNA, the expression of ERK1/2 was detected for understanding the function of the ERK1/2 signal pathway. Three siRNAs for MMP-2 (MMP-2-siRNA) were designed, and the optimal one was selected and tested at different time points of 24, 48 and 72 h. Under an ADM-induced condition, ADM-MG-63 cells were finally stable living in the medium of ADM (200 ng/mL). PD98059 could effectively suppress the expression levels of p-ERK1/2 and MMP-2. When the MMP-2 was silenced by using MMP-2-siRNA, the expression of p-ERK1/2 was enhanced. It is concluded that MMP-2 may be involved in ADM resistance dependent on ERK1/2 signal pathway, suggesting interference in ERK1/2 may be a new method of targeted therapy for tumor resistance.

Objective To analyze the treatment effect and factors affecting the prognosis in young people of papillary thyroid carcinoma (PTC).Methods A total of 69 PTC patients were reviewed retrospectively in Sun Yatsen University Cancer Center from Dec 1996 to Oct 2005.The prognostic status were compared using the Kaplan-Meier methods,the factors were analyzed by Log-rank test and the multiple factors were estimated by the Cox regression models.Results There were 26 males and 43 females.The median age was 24 years (mean:23.1 years).The average follow-up time was 113 months ranging from 73 to 174 months.20.3％ patients (14/69) experienced recurrence during follow-up time and 1.4％ patient (1/69) died.All the patients received levothyroxine after operation.The 10 year disease-free survival (DFS) rate of patients with or without extra thyroidal extension (ETE) of PTC was 29.6％ and 80.1％,respectively (P ＜ 0.01).Multivariate analysis showed that young PTC people with ETE had a worse prognosis (P =0.004).Conclusions Young papillary thyroid carcinoma patients had a favorable outcome.Surgical procedure was the first choice for this kind of patients.ETE was an independent factor for the recurrence of young PTC patients.

24 patients suffering from tertian malaria were treated with 600 mg of artesunate tablet as a total dose in a 5-day course. The result showed that all patients were clinically cured with symptoms and signs subsided quickly. Defervescence and parasite clearance times were 19. 9?15.8 and 56. 8?17. 4 hours respectively. The recrudescent rate was 54.2％ within 28 days.