Humans ; Medical Waste Disposal ; methods ; standards ; Public Health Administration ; legislation & jurisprudence ; standards

Objective To investigate the early diagnostic value of serum Cystatin C in the diagnosis of acute kidney injury (AKI) in critically ill children.Methods Ninety-eight children of the severe case patients' rooms were divided into two groups:33 cases were AKI,65 cases were NAKI.Cystatin C and SCr in blood serum were detected by immunoturbidimetry and enzymic method every day.And compared serum Cystatin C,SCr concentrate and median diagnosis time between the two groups.Results Thirty-three cases in 98 cases of critically ill children occurred the AKI,65 patients without AKI.Comparing with the NAKI,the Cystatin C of AKI patients were significantly higher ((2.68 ±0.86) mg/L vs (0.76 ±0.15) mg/L) and SCr ((209.21 ± 100.53 ) μmol/L vs (77.46 ± 8.11) μmol/L),the differences were statistically significant (t =10.55,17.56,P ＜0.001) ; Cystatin C was in positive correlation with SCr (r =0.874,P ＜ 0.001) ; Median diagnosis time of AKI-Ⅰ,Ⅱ,and Ⅲ stage with Cystatin C were (3.0 ± 2.5) d,(5.0 ± 3.0) d and (9.0 ± 4.0) d,respectively,which was earlier than that of SCr ((5.0 ± 2.0) d,(7.0 ± 2.5) d and (10.0 ± 3.0) d,respectively; t =4.39,2.29,3.16,respectively ;P ＜ 0.01 or P ＜ 0.05).Conclusion In the process of AKI,the level of serum Cystatin C elevated significantly earlier than SCr,so it could as be one of the early dynamic criteria in the critically ill children.

Traditional Chinese Medicine ( TCM) is an important part of primary health care services with the advantages of simplicity, convenience, low costs, and effective examinations. Under the influence of Western Medi-cine, improving the enterprise advantages of TCM becomes an imminent issue to resolve. With the perspective of be-havioral and social sciences, the paper systematically reviews the factors which impact the consumer acceptance of TCM in primary healthcare services and constructs the corresponding structural equation model based on the social learning theory. Meanwhile, AMOS17. 0 and SPSS were used to process empirical data. The paper presents the fol-lowing important conclusions: service encounter quality, emotional well-being and the influence degree are signifi-cantly correlated with the consumer acceptance of TCM;there is no significant relationship between individual-cogni-tion and consumer acceptance of TCM;consumer trust is not related to consumer acceptance of TCM;TCM technolo-gy acceptance is the major source of consumer acceptance of TCM. Finally, this paper introduces the policy recom-mendations of improving and promoting the service quality of TCM.

Fasciolopsis buski speciemens were collected, fixed with neutral formalin fixative solution, stained with alum-carmine staining solution, discolored with 2% kalium alum. The fixed and stained specimen shows clear internal structure with bright color, and can be stored for long time.

Objective To compare the effects of Niaoduqing Tablet and Niaoduqing Granule in tre atingchronic renal failure(CRF)induced by adenine in rats,which is expected to provide some lab oratory data for the clinical applic ation and innovation of the dosage forms.Methods CRF rat models induced by adenine were administered with Niaoduqing Tabl et or Niaoduqing Granule and then the seru m levels of BUN and Scr,haematologic al and biochemical indexes and renal histolog-ical features were observed.Results Both of two forms reduced serum levels of BUN and Scr,ameliorate the electrolyte,increase the number of red blood cell,hemoglobin content and haematocrit and alleviate pathological changes of the kidney tissue.Conclusion Both Niaoduqing Tablet and Niaoduqi ng Granule is effective in treating CRF in rats and the effect of Niaoduqing Tablet in ameliorating a nemia is superior to that of Niaoduqi ng Granule.

Berberine is the major component of Coptidis Rhizoma and it has been used as anti-infection, anti-inflammation drug for gastrointestinal diseases. In recent years, evidence showed that it could regulate glucose and lipid metabolism. Moreover, its activity had been tested by clinical trials and animal researches. The mechanisms of berberine in diabetes include: improving the function of beta-cell; prompting insulin secretion and islets regeneration, lowing lipid level, regulating glucose and lipid metabolic by influence transcriptional factors expression such as PPARgamma, C/EBPalpha, SREBP-1c, LXR, having the activities of anti-oxidation and inhibiting reductase to repress oxidative stress state and regulate metabolic signal pathway. Although numbers of data supported that berberine could improving insulin resistance by clinical trials and animal studies, the large scale, multicenter clinical trials are needed to evaluate the effects of berberine for diabetes and its complications in the time of evidence-based medicine.
Animals ; Berberine ; adverse effects ; therapeutic use ; Diabetes Mellitus, Type 2 ; drug therapy ; genetics ; metabolism ; Glucose ; metabolism ; Humans ; Hypoglycemic Agents ; adverse effects ; therapeutic use ; Insulin ; metabolism ; Lipid Metabolism ; drug effects

Health Facilities ; Humans ; Medical Waste Disposal ; methods ; standards ; Public Health Practice ; standards

Objective To investigate non-psychiatric doctors' knowledge and attitude toward depression. Method Self-made Depressive Knowledge Questionaire (DKQ), which includes 24 items covering general knowledge, symptoms and other related questions of depression, were used in 346 non-psychiatric doctors who came from different grade general hospitals of Hunan. Results Average score of DKQ was 13.53±2.08,and corrective answering rates to different questions were 14.5％～95.4%.There were some associations between DKQ's score and grade of hospitals, subjects' education and attitude to depression. Conclusion It is necessary to re-educate non-psychiatric doctors with depressive knowledge.

Objective To study the effects of supra-physiologic glucose on the expression of PDX-1 and insulin secretion. Methods Islet cells isolated from SD rats were incubated in medium containing 5.6 mmol/L or 33 mmol/L glucose respectively for 3 days. Basic and glucose-induced insulin levels in supernate and in islet cells were detected. The PDX-1 mRNA and protein were detected. Results (1) Islet cells exposed to 33 mmol/L vs 5.6 mmol/L glucose for 3 days showed significant decrease in the basic and glucose-induced insulin release, and in cell insulin content and PDX-1 protein. (2) Prolonging incubation of islet cells in 33 mmol/L glucose aggravated the inhibitive effect on PDX-1 expression and insulin secretion. (3)Recovery to normal concentration of glucose in 3 days could partly improve PDX-1 expression and insulin secretion. Conclusions Inhibition of PDX-1 is one of the mechanism of glucotoxicity, and recovery to normal concentration of glucose in 3 days can partly improve PDX-1 expression and insulin secretion.

Objective To investigate the value of ~(99m)Tc-HL91 hypoxia imaging,~(99m)Tc-MIBI tumoraffin and computered tomography(CT) in the diagnosis of lung cancer.Methods The ~(99m)Tc-HL91 hypoxia imaging,~(99m)Tc-MIBI tumoraffin and CT were performed in 50 patients with lung neoplasm confirmed by pathology.Results There was not significant difference in the sensitivity of three imaging examination methods.The specificity of ~(99m)Tc-HL91 hypoxia imaging was obviously higher than that of ~(99m)Tc-MIBI tumoraffin and CT.The accuracy of ~(99m)Tc-HL91 hypoxia imaging was higher than that of ~(99m)Tc-MIBI tumoraffin,and had not significance difference with that of CT.The sensitivity,specificity and accuracy of the combination of three imaging examination methods in the diagnosis of lung cancer were 96.9%,100% and 98%,respectively.Conclusion The ~(99m)Tc-HL91 hypoxia imaging is valuable in diagnosing lung cancer and the diagnostic accuracy may be further improved with the combination of ~(99m)Tc-MIBI tumoraffin and CT.