Chronic myelomonocytic leukemia (CMML) is a rare malignant disorder of hematopoietic system.It is classified by WHO as MDS/MPN and many gene mutations are invovled such as RAS,TET2 and CBL.Hypomethylating drugs are effective in the management of CMML and may alter the prognosis.Allogeneic stem cell transplantation is still the only way to cure the disorder.

Objective To investigate the influence of ulinastatin combined with Xue Bi Jing injection therapy on serum interleukin interleukin-8 (IL)-8 and γ-interferon (γ-IFN) of patients with severe acute pancreatitis(SAP).Methods Seventy patients with SAP were randomly divided into the control group(n=35) and research group (n =35).Patients in the control group were given ulinastatin on the foundation of traditional treatment,and in research group were given Xue Bi Jing injection on the foundation of treatment of the control group.The rehef time of abdominal distension and abdominal pain,time of serum amylase (sAMY) and white blood cell count(WBC) returned to normal were recorded.The IL-8 and γ-IFN of before and after treatment of the two groups were measured.Results The relief time of abdominal distension,abdominal pain,times of serum amylase,WBC returned to normal in treatment group were (4.1 ± 1.5) d,(4.2 ± 1.9) d,(5.2 ± 1.4) d,(6.5 ± 1.9) d respectively,significantly shorter than the control group((6.2±2.4) d,(6.5±2.2) d,(6.8±2.5) d,(8.9±2.5) d;t=5.468,5.747,4.354,4.647;P＜0.05).The IL-8 and IFN-γ at the 3rd.day and 7th day after treatment of the two groups decreased obviously,and those in the observation group were significantly lower than the control group (P ＜ 0.05).Conclusion Ulinastatin combined with Xue Bi Jing injection therapy can conspicuously reduce the IL-8 and γ-IFN levels in patients with severe acute pancreatitis and alleviate the clinical symptom.

Objective To evaluate the effect of remote ischemic perconditioning on systemic inflammatory response in the patients undergoing cardiac valve replacement with cardiopulmonary bypass (CPB).Methods Forty adult patients undergoing cardiac valve replacement under CPB were randomly divided into 2 groups (n =20 each):control group (group C) and remote ischemic preconditioning group (group R).Anesthesia was induced with iv injection of midazolam,fentanyl,vecuronium.The patients were mechanically ventilated after endotracheal intubation.Anesthesia was maintained with iv injection of midazolam,fentanyl,vecuronium and inhalation of sevoflurane.Three cycles of 5-min ischemia and 5-rmin reperfusion were performed on the fight lower extremity immediately after aortic occlusion by means of a tourniquet in group R.A tourniquet was only placed under the right lower extremity in group C.Before CPB and at 0,6 and 24 h after termination of CPB (T1-4),blood samples were obtained from the right internal jugular vein for determination of levels of serum intercellular adhesion molecule-1 (ICAM-1),IL-6 and TNF-α.Results The levels of serum ICAM-1,IL-6 and TNF-α were significantly lower in group R than in group C (P ＜ 0.05).Compared with the baseline value at T1,the levels of serum ICAM-1,IL-6 and TNF-α were significantly increased at T2 and T3 in both groups,serum ICAM-1 and IL-6 levels were increased at T4 in group C,while serum IL-6 level was increased at T4 in group R (P ＜ 0.05).The levels of serum ICAM-1,IL-6 and TNF-α were significantly decreased at T3 and T4 than at T2 in both group (P ＜ 0.05).The levels of serum ICAM-1,IL-6 and TNF-α were significantly lower at T4 than at T3 in both groups (P ＜ 0.05).Conclusion Remote ischemic perconditioning can alleviate the systemic inflammatory response in patients undergoing cardiac valve replacement with CPB.

OBJECTIVE:To study in vitro effects of tanshinoneⅡA to reverse multiple drug resistance. METHODS:MCF-7/ADM cells and A549/DDP cells were cultured with 0 [20 mg/L doxorubicin(ADM)or cisplatin(DDP),negative control],5 mg/ml tanshinone ⅡA(combined with 20 mg/L ADM or DDP)for 24 and 48 h. Then MTT method was used to determine cell viability, and polymerase chain reaction(PCR)was adopted to detect mRNA expressions of cell cycle control protein CDC25A and cell cy-clin dependent kinase (CKD2). RESULTS:Compared to the negative control,after MCF-7/ADM cells and A549/DDP cells were cultured with tanshinone ⅡA for 24 and 48 h,cell viability was weaker,also were mRNA expressions of CDC25A and CKD2. There were statistical differences(P<0.01). CONCLUSIONS:Tanshinone ⅡA combined with ADM or DDP can inhibit the viabili-ty of cell line MCF-7/ADM and cell line A549/DDP,decrease expression of CDC25A,CKD2 mRNA in cells and reverse multiple drug resistance in malignant tumors.

Objective To investigate the incidence and diagnosis of glucose-6-phosphate dehydrogenate(G-6-PD) deficiency through screening with umbilical blood or infants' peripheral blood.Methods The umbilical blood of 11574 infants delivered in our hospital was detected to screen G-6-PD deficiency.Activity of G-6-PD was also detected in 320 G-6-PD deficiency infants and 215 normal infants from 3 to 5 years old.Results Six hundred and forty-one patients in 11574 cases were screened with umbilical blood as G-6-PD deficiency and the incidence rate was 5.5%.The incidence rates in boys and girls were 7.9% and 3.0% respectively with significant difference(P

Objective To learn how well these items of Hubei Province meet the quality standards of allowed imprecision. Methods Collected the indoor quality control data of median concentration levels from the laboratories which participated the project of interlaboratory comparisons of clinical chemistry indoor quality control data in Hubei Province.This paper was to analyze the variation coefficient of indoor quality control for 21 routine clinical chemistry examination items which were K,Na,Cl,TCa,P,GLu,Urea,UA,Cr,TP,Alb,TC,TG,ALT,AST,TBil,ALP,AMS,CK,LDH andγ-GT.The other objec-tive was to learn how well these items of Hubei Province meet the quality standards of allowed imprecision.Then took the 1/3 TEa,1/4 TEa,WS/T-403-2012 and minimum imprecision derived from biological variation as quality specification.And an-alyzed the percentage of laboratories in meeting the quality standards.Results The TG,ALT,CK and TBil in more than 50% of the participated laboratories could meet the quality standards of the 1/3 TEa,1/4 TEa,WS/T-403-2012 and the low-est appropriate imprecision derived from biological variation.The Cl and Cr in more than 50% of laboratories couldn’t meet the all above quality standards.The Na and TCa in all laboratories couldn’t meet the quality standards of best imprecision derived from biological variation.The evaluation criterion for qualified items setted was that the variation coefficient in more than 80% laboratories was less than the quality standard.Thus,the percentage of the items which meet the lowest quality standard of biological variation and the all 21 items was the most (66.7%).While the percentage of the items which met the quality standards of the WS/T403-2012 and the best biological variation was the least (14.3%).Conclusion In short,the values of indoor variation coefficient of the 21 items in laboratories which participated the project of interlaboratory compari-sons of clinical chemistry indoor quality control data generally met the requirements.But some items had a little higher de-gree of dispersion.The laboratories should set the appropriate imprecision levels based on the detection capability and quality standards and improve the quality of examination through continuous efforts.

Objective: To set up a liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method for determination of betamethasone in rabbit plasma. Methods: Betamethasone and the internal standard A0 were extracted from rabbit plasma by liquid-liquid extraction with diethyl ether-hexane (4:1,V/V). Chromatographic separation was performed on a Zorbax Eclipse XDB-C18 column with a mobile phase consisted of acetonitrile-5 mmol/L ammonium acetate-formic acid (80:20:0.1,V/V/V) at a flow-rate of 0.60 ml/min. A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 393→m/z 373 and m/z 393→ m/z 355 for betamethasone,and m/z 489→m/z 357 for the internal standard. Results: The linear calibration curves were obtained within the concentration range of 15.0-1 000 pg/ml. The lower limit of quantification was 15.0 pg/ml. The intra- and inter-day relative standard deviation over the entire concentration range was less than 13.0%. The accuracy was in the range of -1.4%o to -0.6% in terms of relative error. The method was applied to a pharmacokinetic study of betamethasone dipropionate cream in rabbits. Maximal betamethasone plasma level was observed after (11.0±5.3) h and the Cmax. was (167±28) pg/ml,AUC0-1, was (4.24±1.68) ng · h · ml-1 after percutaneous administration of 0.5 g betamethasone dipropionate. Conclusion: This method is selective and sensitive,and can be used for the purpose of the pharmacokinetic study of betamethasone dipropionate cream.

Objective To explore the role of ppk1 gene in E.coli CFT073 strain during urinary tract infection (UTI). Methods C57BL/6 mouse models of acute UTI with the wild-type(CFT073) and ppk1 mutant (△pk1) infected, were used to compare the bacteria colonization and inflammation induction abilities of bladder tissues. Results In the mouse models, the △pk1 strain showed a significantly lower infection rate (73.3% vs 93.3%) and lower adhesion frequency of bladder (0.01%vs 0.5%) than those of the CFT073 strain. The expression of IL-6 and TNF-αwere reduced in the bladder of △pk1 infected group (P<0.05). Hematoxylin-Eosin tissue staining showed that the damage degree of bladders in △pk1 infected mice were less serious than the CFT073 infected mice. Conclusion ppk1 gene plays an important role in E.coli colonization to bladder and the inflammation induction ability.

Neurogenesis refers to a biological process of neural stem cells self-proliferate and differentiate into new neurons, and are integrated into the neural networks, including the embryonic neurogenesis and adult neurogenesis. Studies have suggested that microRNAs (miRNAs) play vital regulatory roles in neurogenesis. This article reviews the molecular mechanisms of miRNAs in the regulation of embryonic and adult neurogenesis as well as the possible regulatory roles of miRNAs in the process of neurogenesis after cerebral ischemia.